{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:32:19Z","timestamp":1781109139220,"version":"3.54.1"},"reference-count":20,"publisher":"National Academy of Sciences","issue":"suppl_1","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,9,30]]},"abstract":"\n We have tested the ability of bone marrow (BM) cells (BMCs) to form hepatocytes in liver injury models. We used three models: (\n i<\/jats:italic>\n ) carbon tetrachloride (CCl\n 4<\/jats:sub>\n ) treatment, (\n ii<\/jats:italic>\n ) albumin-urokinase transgenic mouse [TgN(Alb1Plau)], and (\n iii<\/jats:italic>\n ) hepatitis B transgenic mouse [TgN(Alb1HBV)]. As a nonselective liver injury model, irradiated C57BL\/6 (B6) mice were transplanted with BMCs from GFP transgenic mouse [TgN(ActbEGFP)] or \u03b2-galactosidase transgenic mouse [TgN(MtnLacZ)] followed by the administration of CCl\n 4<\/jats:sub>\n . Irradiated TgN(Alb1HBV) and TgN(Alb1Plau) were also transplanted with BMCs from TgN(ActbEGFP) or TgN(MtnLacZ). Approximately 1.5 \u00d7 10\n 6<\/jats:sup>\n hepatocytes per liver were analyzed for GFP-positive cells, and the whole livers were inspected for \u03b2-galactosidase expression. No GFP-positive hepatocytes and no gross blue staining of the livers with 5-bromo-4-chloro-3-indolyl \u03b2-\n d<\/jats:sc>\n -galactoside in any of the 18 recipient mice analyzed were detected. The livers from female animals with gender-mismatched BM transplantation were also tested with Y chromosome fluorescent\n in situ<\/jats:italic>\n hybridization analysis to detect donor-derived cells. A total of five isolated hepatocytes were positive for Y chromosome in 4.1 \u00d7 10\n 5<\/jats:sup>\n hepatocytes analyzed. Our results demonstrate that there is little or no contribution of BMCs to the replacement of injured livers in these models. We conclude that BM-derived cells cannot generally lead to a cure of liver damage.\n <\/jats:p>","DOI":"10.1073\/pnas.1834198100","type":"journal-article","created":{"date-parts":[[2003,9,30]],"date-time":"2003-09-30T16:47:00Z","timestamp":1064940420000},"page":"11850-11853","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":125,"title":["Little evidence of bone marrow-derived hepatocytes in the replacement of injured liver"],"prefix":"10.1073","volume":"100","author":[{"given":"Yoshiyuki","family":"Kanazawa","sequence":"first","affiliation":[{"name":"Laboratory of Genetics, The Salk Institute, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inder M.","family":"Verma","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, The Salk Institute, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2003,9,30]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5356.1528"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/43919"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35070587"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.181177898"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI12150"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5497.1779"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5497.1775"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5417.1168"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510310135"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/81326"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-05-1338"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01539"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8108734"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90770-8"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-34509"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1177\/095632020301400101"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00593-6"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.26751"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1079318"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01531"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1834198100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T05:02:19Z","timestamp":1649826139000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1834198100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9,30]]},"references-count":20,"journal-issue":{"issue":"suppl_1","published-print":{"date-parts":[[2003,9,30]]}},"alternative-id":["10.1073\/pnas.1834198100"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1834198100","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,9,30]]},"assertion":[{"value":"2003-09-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}