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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,11,19]]},"abstract":"<jats:p>\n                    Sonic hedgehog (SHH) signaling plays a pivotal role in 2 different phases during brain development. Early SHH signaling derived from the prechordal plate (PrCP) triggers secondary\n                    <jats:italic>Shh<\/jats:italic>\n                    induction in the forebrain, which overlies the PrCP, and the induced SHH signaling, in turn, directs late neuronal differentiation of the forebrain. Consequently,\n                    <jats:italic>Shh<\/jats:italic>\n                    regulation in the PrCP is crucial for initiation of forebrain development. However, no enhancer that regulates prechordal\n                    <jats:italic>Shh<\/jats:italic>\n                    expression has yet been found. Here, we identified a prechordal enhancer, named SBE7, in the vicinity of a cluster of known forebrain enhancers for\n                    <jats:italic>Shh<\/jats:italic>\n                    . This enhancer also directs\n                    <jats:italic>Shh<\/jats:italic>\n                    expression in the ventral midline of the forebrain, which receives the prechordal SHH signal. Thus, the identified enhancer acts not only for the initiation of\n                    <jats:italic>Shh<\/jats:italic>\n                    regulation in the PrCP but also for subsequent\n                    <jats:italic>Shh<\/jats:italic>\n                    induction in the forebrain. Indeed, removal of the enhancer from the mouse genome markedly down-regulated the expression of\n                    <jats:italic>Shh<\/jats:italic>\n                    in the rostral domains of the axial mesoderm and in the ventral midline of the forebrain and hypothalamus in the mouse embryo, and caused a craniofacial abnormality similar to human holoprosencephaly (HPE). These findings demonstrate that SHH signaling mediated by the newly identified enhancer is essential for development and growth of the ventral midline of the forebrain and hypothalamus. Understanding of the\n                    <jats:italic>Shh<\/jats:italic>\n                    regulation governed by this prechordal and brain enhancer provides an insight into the mechanism underlying craniofacial morphogenesis and the etiology of HPE.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1901732116","type":"journal-article","created":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T21:30:32Z","timestamp":1572903032000},"page":"23636-23642","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":48,"title":["SHH signaling mediated by a prechordal and brain enhancer controls forebrain organization"],"prefix":"10.1073","volume":"116","author":[{"given":"Tomoko","family":"Sagai","sequence":"first","affiliation":[{"name":"Mammalian Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima 411-8540, Japan;"},{"name":"Information Resource Research Center, Association for Propagation of the Knowledge of Genetics, Mishima 411-8540, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takanori","family":"Amano","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima 411-8540, Japan;"},{"name":"Next Generation Human Disease Model Team, RIKEN BioResource Research Center, Tsukuba 305-0074, Japan;"},{"name":"Department of Genetics, SOKENDAI, Mishima 411-8540, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0235-3478","authenticated-orcid":false,"given":"Akiteru","family":"Maeno","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima 411-8540, Japan;"},{"name":"Plant Cytogenetics Laboratory, National Institute of Genetics, Mishima 411-8540, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rieko","family":"Ajima","sequence":"additional","affiliation":[{"name":"Department of Genetics, SOKENDAI, Mishima 411-8540, Japan;"},{"name":"Mammalian Development Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima 411-8540, Japan;"},{"name":"Mouse Research Supporting Unit, National Institute of Genetics, Mishima 411-8540, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Shiroishi","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima 411-8540, Japan;"},{"name":"Department of Genetics, SOKENDAI, Mishima 411-8540, Japan;"},{"name":"RIKEN BioResource Research Center, Tsukuba 305-0074, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2019,11,4]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1242\/dev.126.13.2921","article-title":"Axial mesendoderm refines rostrocaudal pattern in the chick nervous system","volume":"126","author":"Rowan A. M.","year":"1999","unstructured":"A. M. Rowan, C. D. Stern, K. G. Storey, Axial mesendoderm refines rostrocaudal pattern in the chick nervous system. Development 126, 2921\u20132934 (1999).","journal-title":"Development"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1126\/science.274.5290.1115","article-title":"Diversity and pattern in the developing spinal cord","volume":"274","author":"Tanabe Y.","year":"1996","unstructured":"Y. Tanabe, T. M. Jessell, Diversity and pattern in the developing spinal cord. Science 274, 1115\u20131123 (1996).","journal-title":"Science"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/383407a0","article-title":"Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function","volume":"383","author":"Chiang C.","year":"1996","unstructured":"C. Chiang ., Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function. Nature 383, 407\u2013413 (1996).","journal-title":"Nature"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"4153","DOI":"10.1242\/dev.124.20.4153","article-title":"Patterning of the chick forebrain anlage by the prechordal plate","volume":"124","author":"Pera E. M.","year":"1997","unstructured":"E. M. Pera, M. Kessel, Patterning of the chick forebrain anlage by the prechordal plate. Development 124, 4153\u20134162 (1997).","journal-title":"Development"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"2709","DOI":"10.1242\/dev.124.14.2709","article-title":"Inductive interactions direct early regionalization of the mouse forebrain","volume":"124","author":"Shimamura K.","year":"1997","unstructured":"K. Shimamura, J. L. Rubenstein, Inductive interactions direct early regionalization of the mouse forebrain. Development 124, 2709\u20132718 (1997).","journal-title":"Development"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0092-8674(00)80334-7","article-title":"Cooperation of BMP7 and SHH in the induction of forebrain ventral midline cells by prechordal mesoderm","volume":"90","author":"Dale J. K.","year":"1997","unstructured":"J. K. Dale ., Cooperation of BMP7 and SHH in the induction of forebrain ventral midline cells by prechordal mesoderm. Cell 90, 257\u2013269 (1997).","journal-title":"Cell"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1242\/dev.126.2.281","article-title":"Regionalization of Sonic hedgehog transcription along the anteroposterior axis of the mouse central nervous system is regulated by Hnf3-dependent and -independent mechanisms","volume":"126","author":"Epstein D. J.","year":"1999","unstructured":"D. J. Epstein, A. P. McMahon, A. L. Joyner, Regionalization of Sonic hedgehog transcription along the anteroposterior axis of the mouse central nervous system is regulated by Hnf3-dependent and -independent mechanisms. Development 126, 281\u2013292 (1999).","journal-title":"Development"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/S0092-8674(00)81437-3","article-title":"FGF and Shh signals control dopaminergic and serotonergic cell fate in the anterior neural plate","volume":"93","author":"Ye W.","year":"1998","unstructured":"W. Ye, K. Shimamura, J. L. Rubenstein, M. A. Hynes, A. Rosenthal, FGF and Shh signals control dopaminergic and serotonergic cell fate in the anterior neural plate. Cell 93, 755\u2013766 (1998).","journal-title":"Cell"},{"key":"e_1_3_4_9_2","first-page":"809","article-title":"Sonic hedgehog: A common signal for ventral patterning along the rostrocaudal axis of the neural tube","volume":"39","author":"Ericson J.","year":"1995","unstructured":"J. Ericson, J. Muhr, T. M. Jessell, T. Edlund, Sonic hedgehog: A common signal for ventral patterning along the rostrocaudal axis of the neural tube. Int. J. Dev. Biol. 39, 809\u2013816 (1995).","journal-title":"Int. J. Dev. Biol."},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/0092-8674(95)90536-7","article-title":"Sonic hedgehog induces the differentiation of ventral forebrain neurons: A common signal for ventral patterning within the neural tube","volume":"81","author":"Ericson J.","year":"1995","unstructured":"J. Ericson ., Sonic hedgehog induces the differentiation of ventral forebrain neurons: A common signal for ventral patterning within the neural tube. Cell 81, 747\u2013756 (1995).","journal-title":"Cell"},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/375322a0","article-title":"Requirement of 19K form of Sonic hedgehog for induction of distinct ventral cell types in CNS explants","volume":"375","author":"Mart\u00ed E.","year":"1995","unstructured":"E. Mart\u00ed, D. A. Bumcrot, R. Takada, A. P. McMahon, Requirement of 19K form of Sonic hedgehog for induction of distinct ventral cell types in CNS explants. Nature 375, 322\u2013325 (1995).","journal-title":"Nature"},{"key":"e_1_3_4_12_2","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/0092-8674(95)90397-6","article-title":"Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis","volume":"81","author":"Roelink H.","year":"1995","unstructured":"H. Roelink ., Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis. Cell 81, 445\u2013455 (1995).","journal-title":"Cell"},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.ydbio.2008.11.022","article-title":"Mouse Shh is required for prechordal plate maintenance during brain and craniofacial morphogenesis","volume":"327","author":"Aoto K.","year":"2009","unstructured":"K. Aoto ., Mouse Shh is required for prechordal plate maintenance during brain and craniofacial morphogenesis. Dev. Biol. 327, 106\u2013120 (2009).","journal-title":"Dev. Biol."},{"key":"e_1_3_4_14_2","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1242\/dev.124.3.603","article-title":"A single morphogenetic field gives rise to two retina primordia under the influence of the prechordal plate","volume":"124","author":"Li H.","year":"1997","unstructured":"H. Li, C. Tierney, L. Wen, J. Y. Wu, Y. Rao, A single morphogenetic field gives rise to two retina primordia under the influence of the prechordal plate. Development 124, 603\u2013615 (1997).","journal-title":"Development"},{"key":"e_1_3_4_15_2","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1016\/S0960-9822(99)80363-7","article-title":"Spatially distinct head and heart inducers within the Xenopus organizer region","volume":"9","author":"Schneider V. A.","year":"1999","unstructured":"V. A. Schneider, M. Mercola, Spatially distinct head and heart inducers within the Xenopus organizer region. Curr. Biol. 9, 800\u2013809 (1999).","journal-title":"Curr. Biol."},{"key":"e_1_3_4_16_2","doi-asserted-by":"crossref","first-page":"160197","DOI":"10.1098\/rsob.160197","article-title":"SBE6: A novel long-range enhancer involved in driving sonic hedgehog expression in neural progenitor cells","volume":"6","author":"Benabdallah N. S.","year":"2016","unstructured":"N. S. Benabdallah ., SBE6: A novel long-range enhancer involved in driving sonic hedgehog expression in neural progenitor cells. Open Biol. 6, 160197 (2016).","journal-title":"Open Biol."},{"key":"e_1_3_4_17_2","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1242\/dev.02239","article-title":"A functional screen for sonic hedgehog regulatory elements across a 1 Mb interval identifies long-range ventral forebrain enhancers","volume":"133","author":"Jeong Y.","year":"2006","unstructured":"Y. Jeong, A functional screen for sonic hedgehog regulatory elements across a 1 Mb interval identifies long-range ventral forebrain enhancers. Development 133, 761\u2013772 (2006).","journal-title":"Development"},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/ng.3542","article-title":"Cis-regulatory architecture of a brain signaling center predates the origin of chordates","volume":"48","author":"Yao Y.","year":"2016","unstructured":"Y. Yao ., Cis-regulatory architecture of a brain signaling center predates the origin of chordates. Nat. Genet. 48, 575\u2013580 (2016).","journal-title":"Nat. Genet."},{"key":"e_1_3_4_19_2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.mod.2013.09.003","article-title":"A novel regulatory element for Shh expression in the lung and gut of mouse embryos","volume":"131","author":"Tsukiji N.","year":"2014","unstructured":"N. Tsukiji, T. Amano, T. Shiroishi, A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131, 127\u2013136 (2014).","journal-title":"Mech. Dev."},{"key":"e_1_3_4_20_2","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1242\/dev.058917","article-title":"Spatial and temporal requirements for sonic hedgehog in the regulation of thalamic interneuron identity","volume":"138","author":"Jeong Y.","year":"2011","unstructured":"Y. Jeong ., Spatial and temporal requirements for sonic hedgehog in the regulation of thalamic interneuron identity. Development 138, 531\u2013541 (2011).","journal-title":"Development"},{"key":"e_1_3_4_21_2","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1038\/ng.230","article-title":"Regulation of a remote Shh forebrain enhancer by the Six3 homeoprotein","volume":"40","author":"Jeong Y.","year":"2008","unstructured":"Y. Jeong ., Regulation of a remote Shh forebrain enhancer by the Six3 homeoprotein. Nat. Genet. 40, 1348\u20131353 (2008).","journal-title":"Nat. Genet."},{"key":"e_1_3_4_22_2","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.devcel.2011.12.023","article-title":"Disruption of SoxB1-dependent Sonic hedgehog expression in the hypothalamus causes septo-optic dysplasia","volume":"22","author":"Zhao L.","year":"2012","unstructured":"L. Zhao ., Disruption of SoxB1-dependent Sonic hedgehog expression in the hypothalamus causes septo-optic dysplasia. Dev. Cell 22, 585\u2013596 (2012).","journal-title":"Dev. Cell"},{"key":"e_1_3_4_23_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.devcel.2016.10.015","article-title":"The Shh topological domain facilitates the action of remote enhancers by reducing the effects of genomic distances","volume":"39","author":"Symmons O.","year":"2016","unstructured":"O. Symmons ., The Shh topological domain facilitates the action of remote enhancers by reducing the effects of genomic distances. Dev. Cell 39, 529\u2013543 (2016).","journal-title":"Dev. Cell"},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1148\/rg.351140040","article-title":"Holoprosencephaly: A survey of the entity, with embryology and fetal imaging","volume":"35","author":"Winter T. C.","year":"2015","unstructured":"T. C. Winter, A. M. Kennedy, P. J. Woodward, Holoprosencephaly: A survey of the entity, with embryology and fetal imaging. Radiographics 35, 275\u2013290 (2015).","journal-title":"Radiographics"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1101\/gr.229102","article-title":"The human genome browser at UCSC","volume":"12","author":"Kent W. J.","year":"2002","unstructured":"W. J. Kent ., The human genome browser at UCSC. Genome Res. 12, 996\u20131006 (2002).","journal-title":"Genome Res."},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nature11247","article-title":"An integrated encyclopedia of DNA elements in the human genome","volume":"489","author":"E. P. Consortium; ENCODE Project Consortium","year":"2012","unstructured":"E. P. Consortium; ENCODE Project Consortium, An integrated encyclopedia of DNA elements in the human genome. Nature 489, 57\u201374 (2012).","journal-title":"Nature"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1016\/0092-8674(93)90627-3","article-title":"Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity","volume":"75","author":"Echelard Y.","year":"1993","unstructured":"Y. Echelard ., Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell 75, 1417\u20131430 (1993).","journal-title":"Cell"},{"key":"e_1_3_4_28_2","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.devcel.2008.07.003","article-title":"Haploinsufficiency of Six3 fails to activate Sonic hedgehog expression in the ventral forebrain and causes holoprosencephaly","volume":"15","author":"Geng X.","year":"2008","unstructured":"X. Geng ., Haploinsufficiency of Six3 fails to activate Sonic hedgehog expression in the ventral forebrain and causes holoprosencephaly. Dev. Cell 15, 236\u2013247 (2008).","journal-title":"Dev. Cell"},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"D260","DOI":"10.1093\/nar\/gkx1126","article-title":"JASPAR 2018: Update of the open-access database of transcription factor binding profiles and its web framework","volume":"46","author":"Khan A.","year":"2018","unstructured":"A. Khan ., JASPAR 2018: Update of the open-access database of transcription factor binding profiles and its web framework. Nucleic Acids Res. 46, D260\u2013D266 (2018).","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"5185","DOI":"10.1242\/dev.02094","article-title":"Directed differentiation of neural cells to hypothalamic dopaminergic neurons","volume":"132","author":"Ohyama K.","year":"2005","unstructured":"K. Ohyama, P. Ellis, S. Kimura, M. Placzek, Directed differentiation of neural cells to hypothalamic dopaminergic neurons. Development 132, 5185\u20135197 (2005).","journal-title":"Development"},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1159\/000114856","article-title":"Septo-optic dysplasia\u2013Novel insights into the aetiology","volume":"69","author":"Kelberman D.","year":"2008","unstructured":"D. Kelberman, M. T. Dattani, Septo-optic dysplasia\u2013Novel insights into the aetiology. Horm. Res. 69, 257\u2013265 (2008).","journal-title":"Horm. Res."},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"3946","DOI":"10.1093\/hmg\/ddw235","article-title":"Zic2 mutation causes holoprosencephaly via disruption of NODAL signalling","volume":"25","author":"Houtmeyers R.","year":"2016","unstructured":"R. Houtmeyers ., Zic2 mutation causes holoprosencephaly via disruption of NODAL signalling. Hum. Mol. Genet. 25, 3946\u20133959 (2016).","journal-title":"Hum. Mol. Genet."},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"D334","DOI":"10.2741\/Nanni","article-title":"Molecular genetics of holoprosencephaly","volume":"5","author":"Nanni L.","year":"2000","unstructured":"L. Nanni, R. L. Schelper, M. T. Muenke, Molecular genetics of holoprosencephaly. Front. Biosci. 5, D334\u2013D342 (2000).","journal-title":"Front. Biosci."},{"key":"e_1_3_4_34_2","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1038\/ejhg.2010.70","article-title":"The unfolding clinical spectrum of holoprosencephaly due to mutations in SHH, ZIC2, SIX3 and TGIF genes","volume":"18","author":"Paulussen A. D.","year":"2010","unstructured":"A. D. Paulussen ., The unfolding clinical spectrum of holoprosencephaly due to mutations in SHH, ZIC2, SIX3 and TGIF genes. Eur. J. Hum. Genet. 18, 999\u20131005 (2010).","journal-title":"Eur. J. Hum. Genet."},{"key":"e_1_3_4_35_2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1002\/ajmg.c.31615","article-title":"Holoprosencephaly in the genomics era","volume":"178","author":"Roessler E.","year":"2018","unstructured":"E. Roessler, P. Hu, M. Muenke, Holoprosencephaly in the genomics era. Am. J. Med. Genet. C. Semin. Med. Genet. 178, 165\u2013174 (2018).","journal-title":"Am. J. Med. Genet. C. Semin. Med. Genet."},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"e1002524","DOI":"10.1371\/journal.pgen.1002524","article-title":"Loss of Tgif function causes holoprosencephaly by disrupting the SHH signaling pathway","volume":"8","author":"Taniguchi K.","year":"2012","unstructured":"K. Taniguchi, A. E. Anderson, A. E. Sutherland, D. Wotton, Loss of Tgif function causes holoprosencephaly by disrupting the SHH signaling pathway. PLoS Genet. 8, e1002524 (2012).","journal-title":"PLoS Genet."},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/ejhg.2009.125","article-title":"Septo-optic dysplasia","volume":"18","author":"Webb E. A.","year":"2010","unstructured":"E. A. Webb, M. T. Dattani, Septo-optic dysplasia. Eur. J. Hum. Genet. 18, 393\u2013397 (2010).","journal-title":"Eur. J. Hum. Genet."},{"key":"e_1_3_4_38_2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s11102-007-0055-5","article-title":"Genetics of septo-optic dysplasia","volume":"10","author":"Kelberman D.","year":"2007","unstructured":"D. Kelberman, M. T. Dattani, Genetics of septo-optic dysplasia. Pituitary 10, 393\u2013407 (2007).","journal-title":"Pituitary"},{"key":"e_1_3_4_39_2","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1002\/dvg.20356","article-title":"Foxb1-driven Cre expression in somites and the neuroepithelium of diencephalon, brainstem, and spinal cord","volume":"45","author":"Zhao T.","year":"2007","unstructured":"T. Zhao, X. Zhou, N. Szab\u00f3, M. Leitges, G. Alvarez-Bolado, Foxb1-driven Cre expression in somites and the neuroepithelium of diencephalon, brainstem, and spinal cord. Genesis 45, 781\u2013787 (2007).","journal-title":"Genesis"},{"key":"e_1_3_4_40_2","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1002\/cne.21529","article-title":"Fate mapping Nkx2.1-lineage cells in the mouse telencephalon","volume":"506","author":"Xu Q.","year":"2008","unstructured":"Q. Xu, M. Tam, S. A. Anderson, Fate mapping Nkx2.1-lineage cells in the mouse telencephalon. J. Comp. Neurol. 506, 16\u201329 (2008).","journal-title":"J. Comp. Neurol."},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1038\/nn.2545","article-title":"A genomic atlas of mouse hypothalamic development","volume":"13","author":"Shimogori T.","year":"2010","unstructured":"T. Shimogori ., A genomic atlas of mouse hypothalamic development. Nat. Neurosci. 13, 767\u2013775 (2010).","journal-title":"Nat. Neurosci."},{"key":"e_1_3_4_42_2","doi-asserted-by":"crossref","first-page":"6989","DOI":"10.1523\/JNEUROSCI.1089-09.2009","article-title":"Role of neuroepithelial Sonic hedgehog in hypothalamic patterning","volume":"29","author":"Szab\u00f3 N. E.","year":"2009","unstructured":"N. E. Szab\u00f3 ., Role of neuroepithelial Sonic hedgehog in hypothalamic patterning. J. Neurosci. 29, 6989\u20137002 (2009).","journal-title":"J. Neurosci."},{"key":"e_1_3_4_43_2","doi-asserted-by":"crossref","first-page":"156","DOI":"10.3389\/fnana.2014.00156","article-title":"Sonic hedgehog signaling in the development of the mouse hypothalamus","volume":"8","author":"Blaess S.","year":"2015","unstructured":"S. Blaess, N. Szab\u00f3, R. Haddad-T\u00f3volli, X. Zhou, G. \u00c1lvarez-Bolado, Sonic hedgehog signaling in the development of the mouse hypothalamus. Front. Neuroanat. 8, 156 (2015).","journal-title":"Front. Neuroanat."},{"key":"e_1_3_4_44_2","doi-asserted-by":"crossref","first-page":"4462","DOI":"10.1242\/dev.132142","article-title":"Six3 dosage mediates the pathogenesis of holoprosencephaly","volume":"143","author":"Geng X.","year":"2016","unstructured":"X. Geng, S. Acosta, O. Lagutin, H. J. Gil, G. Oliver, Six3 dosage mediates the pathogenesis of holoprosencephaly. 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