{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:14:52Z","timestamp":1791080092596,"version":"4.1.0"},"reference-count":61,"publisher":"National Academy of Sciences","issue":"19","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,9,12]]},"abstract":"<jats:p>\n                    According to Khan\n                    <jats:italic>et al.<\/jats:italic>\n                    [Khan, A. U.,\n Kovacic, D., Kolbanovskiy, A., Desai, M., Frenkel, K. &amp; Geacintov,\n N. E. (2000)\n                    <jats:italic>Proc. Natl. Acad. Sci. USA<\/jats:italic>\n                    97,\n 2984\u20132989], peroxynitrite (ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) decomposes after\n protonation to singlet oxygen\n (\n                    <jats:sup>1<\/jats:sup>\n                    \u0394\n                    <jats:sub>g<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) and singlet oxonitrate\n (nitroxyl,\n                    <jats:sup>1<\/jats:sup>\n                    NO\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) in high yield. They claimed\n to have observed nitrosyl hemoglobin from the reaction of\n NO\n                    <jats:sup>\u2212<\/jats:sup>\n                    with methemoglobin; however, contamination with\n hydrogen peroxide gave rise to ferryl hemoglobin, the spectrum of which\n was mistakenly assigned to nitrosyl hemoglobin. We have carried out\n UV\u2013visible and EPR experiments with methemoglobin and hydrogen\n peroxide-free peroxynitrite and find that no NO\n                    <jats:sup>\u2212<\/jats:sup>\n                    is\n formed. With this peroxynitrite preparation, no light emission from\n singlet oxygen at 1270 nm is observed, nor is singlet oxygen chemically\n trapped; however, singlet oxygen was trapped when hydrogen peroxide was\n also present, as previously described [Di Mascio, P., Bechara, E.\n J. H., Medeiros, M. H. G., Briviba, K. &amp; Sies, H. (1994)\n                    <jats:italic>FEBS Lett.<\/jats:italic>\n                    355, 287\u2013289]. Quantum mechanical and\n thermodynamic calculations show that formation of the postulated\n intermediate, a cyclic form of peroxynitrous acid (trioxazetidine), and\n the products\n                    <jats:sup>1<\/jats:sup>\n                    NO\n                    <jats:sup>\u2212<\/jats:sup>\n                    and\n                    <jats:sup>1<\/jats:sup>\n                    \u0394\n                    <jats:sub>g<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    requires Gibbs energies of\n                    <jats:italic>ca.<\/jats:italic>\n                    +415 kJ\u22c5mol\n                    <jats:sup>\u22121<\/jats:sup>\n                    and\n                    <jats:italic>ca.<\/jats:italic>\n                    +180 kJ\u22c5mol\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively. Our\n results show that the results of Khan\n                    <jats:italic>et al.<\/jats:italic>\n                    are best\n explained by interference from contaminating hydrogen peroxide left\n from the synthesis of peroxynitrite.\n                  <\/jats:p>","DOI":"10.1073\/pnas.190256897","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:40:52Z","timestamp":1027680052000},"page":"10307-10312","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":80,"title":["Peroxynitrite does not decompose to singlet oxygen (\n                    <sup>1<\/sup>\n                    \u0394\n                    <sub>g<\/sub>\n                    O\n                    <sub>2<\/sub>\n                    ) and nitroxyl (NO\n                    <sup>\u2212<\/sup>\n                    )"],"prefix":"10.1073","volume":"97","author":[{"given":"Glaucia R.","family":"Martinez","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Di Mascio","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo G.","family":"Bonini","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ohara","family":"Augusto","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karlis","family":"Briviba","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Sies","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Maurer","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"R\u00f6thlisberger","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Herold","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem H.","family":"Koppenol","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Qu\u00edmica,\r Universidade de S\u00e3o Paulo, CP 26077, CEP 05513\u2013970, S\u00e3o\r Paulo, SP, Brazil; Institut f\u00fcr Physiologische\r Chemie I, Heinrich Heine Universit\u00e4t, Postfach 101700, D-40001\r D\u00fcsseldorf, Germany; and Laboratorium f\u00fcr\r Anorganische Chemie, Eidgen\u00f6ssische Technische Hochschule,\r CH-8092 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2000,9,5]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.5.C1424"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx970200x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.6.3465"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1997.0451"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx980043h"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja980871x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja982887t"},{"key":"e_1_3_3_8_2","first-page":"597","volume-title":"Metal Ions in Biological Systems","author":"Koppenol W H","year":"1999","unstructured":"W H Koppenol Metal Ions in Biological Systems, eds A Sigel, H Sigel (Dekker, New York), pp. 597\u2013619 (1999)."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.2984"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/bio.1170100604"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.4.1620"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(97)00365-1"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx970160x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(96)69030-2"},{"key":"e_1_3_3_15_2","volume-title":"J. 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