{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:33:41Z","timestamp":1790703221835,"version":"4.1.0"},"reference-count":62,"publisher":"National Academy of Sciences","issue":"27","license":[{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,7,2]]},"abstract":"<jats:p>\n                    Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment.\n                    <jats:italic>Shigella flexneri<\/jats:italic>\n                    invades and subverts the human colonic epithelium by the injection of virulence factors through a type 3 secretion system (T3SS). In this work, we report the multiple effects of two\n                    <jats:italic>S. flexneri<\/jats:italic>\n                    effectors, IpaJ and VirA, which target small GTPases of the Arf and Rab families, consequently inhibiting several intracellular trafficking pathways. IpaJ and VirA induce large-scale impairment of host protein secretion and block the recycling of surface receptors. Moreover, these two effectors decrease clathrin-dependent and -independent endocytosis. Therefore,\n                    <jats:italic>S. flexneri<\/jats:italic>\n                    infection induces a global blockage of host cell intracellular transport, affecting the exchange between cells and their external environment. The combined action of these effectors disorganizes the epithelial cell polarity, disturbs epithelial barrier integrity, promotes multiple invasion events, and enhances the pathogen capacity to penetrate into the colonic tissue in vivo.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1902922116","type":"journal-article","created":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T20:49:29Z","timestamp":1560804569000},"page":"13582-13591","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":30,"title":["<i>Shigella<\/i>\n                    promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport"],"prefix":"10.1073","volume":"116","author":[{"given":"Mariana L.","family":"Ferrari","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75015 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Malard\u00e9","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75015 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Grassart","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75015 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Salavessa","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75015 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"},{"name":"Universit\u00e9 Paris Sud, Paris-Saclay University, 91400 Orsay, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Nigro","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75015 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Descorps-Declere","sequence":"additional","affiliation":[{"name":"Institut Pasteur\u2013Hub Bioinformatique et Biostatistique\u2013Centre de Bioinformatique, Biostatistique et Biologie Int\u00e9grative, Unit\u00e9 de Services et de Recherche 3756 IP CNRS, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Rohde","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, B3H 4R2, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4292-1257","authenticated-orcid":false,"given":"Pamela","family":"Schnupf","sequence":"additional","affiliation":[{"name":"Institut Necker Enfants Malades, INSERM\u2013CNRS, Laboratory of Host\u2013Microbiota Interaction, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Masson","sequence":"additional","affiliation":[{"name":"Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrom\u00e9trie de Masse Prot\u00e9omique, 75248 Paris Cedex 05, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Arras","sequence":"additional","affiliation":[{"name":"Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrom\u00e9trie de Masse Prot\u00e9omique, 75248 Paris Cedex 05, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9111-8842","authenticated-orcid":false,"given":"Damarys","family":"Loew","sequence":"additional","affiliation":[{"name":"Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrom\u00e9trie de Masse Prot\u00e9omique, 75248 Paris Cedex 05, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe J.","family":"Sansonetti","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75015 Paris, France;"},{"name":"INSERM U1202, 75015 Paris, France;"},{"name":"Chaire de Microbiologie et Maladies Infectieuses, Coll\u00e8ge de France, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Sauvonnet","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 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EMBO J. 16, 2717\u20132729 (1997).","journal-title":"EMBO J."},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.15252\/embj.201694613","article-title":"Membrane fission by dynamin: What we know and what we need to know","volume":"35","author":"Antonny B.","year":"2016","unstructured":"B. Antonny ., Membrane fission by dynamin: What we know and what we need to know. EMBO J. 35, 2270\u20132284 (2016).","journal-title":"EMBO J."},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1016\/j.celrep.2018.01.039","article-title":"Quantitative and statistical study of the dynamics of clathrin-dependent and -independent endocytosis reveal a differential role of endophilin A2","volume":"22","author":"Bertot L.","year":"2018","unstructured":"L. Bertot ., Quantitative and statistical study of the dynamics of clathrin-dependent and -independent endocytosis reveal a differential role of endophilin A2. Cell Rep. 22, 1574\u20131588 (2018).","journal-title":"Cell Rep."},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"3309","DOI":"10.1242\/jcs.114.18.3309","article-title":"Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells","volume":"114","author":"Wang E.","year":"2001","unstructured":"E. Wang, J. G. Pennington, J. R. Goldenring, W. Hunziker, K. W. Dunn, Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells. J Cell Sci. 114, 3309\u20133321 (2001).","journal-title":"J Cell Sci."},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"E3282","DOI":"10.1073\/pnas.1509091112","article-title":"Bioimage analysis of Shigella infection reveals targeting of colonic crypts","volume":"112","author":"Arena E. T.","year":"2015","unstructured":"E. T. Arena ., Bioimage analysis of Shigella infection reveals targeting of colonic crypts. Proc. Natl. 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Frankel, Enterohaemorrhagic Escherichia coli inhibits recycling endosome function and trafficking of surface receptors. Cell. Microbiol. 16, 1693\u20131705 (2014).","journal-title":"Cell. Microbiol."},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/nrm2755","article-title":"Pathways and mechanisms of endocytic recycling","volume":"10","author":"Grant B. D.","year":"2009","unstructured":"B. D. Grant, J. G. Donaldson, Pathways and mechanisms of endocytic recycling. Nat. Rev. Mol. Cell Biol. 10, 597\u2013608 (2009).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"3399","DOI":"10.1016\/j.jmb.2016.05.023","article-title":"Enterohaemorrhagic E. coli modulates an ARF6:Rab35 signaling axis to prevent recycling endosome maturation during infection","volume":"428","author":"Furniss R. C. D.","year":"2016","unstructured":"R. C. D. Furniss, S. Slater, G. Frankel, A. 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Sci."},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"7661","DOI":"10.1128\/jb.174.23.7661-7669.1992","article-title":"MxiJ, a lipoprotein involved in secretion of Shigella Ipa invasins, is homologous to YscJ, a secretion factor of the Yersinia Yop proteins","volume":"174","author":"Allaoui A.","year":"1992","unstructured":"A. Allaoui, P. J. Sansonetti, C. Parsot, MxiJ, a lipoprotein involved in secretion of Shigella Ipa invasins, is homologous to YscJ, a secretion factor of the Yersinia Yop proteins. J. Bacteriol. 174, 7661\u20137669 (1992).","journal-title":"J. Bacteriol."},{"key":"e_1_3_4_42_2","doi-asserted-by":"crossref","first-page":"2493","DOI":"10.1534\/g3.114.014381","article-title":"A Shigella flexneri virulence plasmid encoded factor controls production of outer membrane vesicles","volume":"4","author":"Sidik S.","year":"2014","unstructured":"S. Sidik ., A Shigella flexneri virulence plasmid encoded factor controls production of outer membrane vesicles. G3 (Bethesda) 4, 2493\u20132503 (2014).","journal-title":"G3 (Bethesda)"},{"key":"e_1_3_4_43_2","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1128\/iai.61.5.1707-1714.1993","article-title":"Characterization of the Shigella flexneri ipgD and ipgF genes, which are located in the proximal part of the mxi locus","volume":"61","author":"Allaoui A.","year":"1993","unstructured":"A. Allaoui, R. M\u00e9nard, P. J. Sansonetti, C. Parsot, Characterization of the Shigella flexneri ipgD and ipgF genes, which are located in the proximal part of the mxi locus. Infect. Immun. 61, 1707\u20131714 (1993).","journal-title":"Infect. Immun."},{"key":"e_1_3_4_44_2","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1038\/embor.2008.28","article-title":"Clathrin-independent endocytosis used by the IL-2 receptor is regulated by Rac1, Pak1 and Pak2","volume":"9","author":"Grassart A.","year":"2008","unstructured":"A. Grassart, A. Dujeancourt, P. B. Lazarow, A. Dautry-Varsat, N. Sauvonnet, Clathrin-independent endocytosis used by the IL-2 receptor is regulated by Rac1, Pak1 and Pak2. EMBO Rep. 9, 356\u2013362 (2008).","journal-title":"EMBO Rep."},{"key":"e_1_3_4_45_2","doi-asserted-by":"crossref","first-page":"3238","DOI":"10.1242\/jcs.188870","article-title":"Microtubule-independent secretion requires functional maturation of Golgi elements","volume":"129","author":"Fourriere L.","year":"2016","unstructured":"L. Fourriere, S. Divoux, M. Roceri, F. Perez, G. Boncompain, Microtubule-independent secretion requires functional maturation of Golgi elements. J. Cell Sci. 129, 3238\u20133250 (2016).","journal-title":"J. Cell Sci."},{"key":"e_1_3_4_46_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1128\/iai.46.1.251-259.1984","article-title":"Cloning and expression of an afimbrial adhesin (AFA-I) responsible for P blood group-independent, mannose-resistant hemagglutination from a pyelonephritic Escherichia coli strain","volume":"46","author":"Labigne-Roussel A. F.","year":"1984","unstructured":"A. F. Labigne-Roussel, D. Lark, G. Schoolnik, S. Falkow, Cloning and expression of an afimbrial adhesin (AFA-I) responsible for P blood group-independent, mannose-resistant hemagglutination from a pyelonephritic Escherichia coli strain. Infect. Immun. 46, 251\u2013259 (1984).","journal-title":"Infect. Immun."},{"key":"e_1_3_4_47_2","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1046\/j.1365-2958.1996.00120.x","article-title":"Bacterial genetics by flow cytometry: Rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction","volume":"22","author":"Valdivia R. H.","year":"1996","unstructured":"R. H. Valdivia, S. Falkow, Bacterial genetics by flow cytometry: Rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction. Mol. Microbiol. 22, 367\u2013378 (1996).","journal-title":"Mol. Microbiol."},{"key":"e_1_3_4_48_2","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/S0014-5793(03)00856-1","article-title":"Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria","volume":"552","author":"S\u00f6rensen M.","year":"2003","unstructured":"M. S\u00f6rensen ., Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria. FEBS Lett. 552, 110\u2013114 (2003).","journal-title":"FEBS Lett."},{"key":"e_1_3_4_49_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.chom.2014.01.005","article-title":"A fluorescent reporter reveals on\/off regulation of the Shigella type III secretion apparatus during entry and cell-to-cell spread","volume":"15","author":"Campbell-Valois F.-X.","year":"2014","unstructured":"F.-X. Campbell-Valois ., A fluorescent reporter reveals on\/off regulation of the Shigella type III secretion apparatus during entry and cell-to-cell spread. Cell Host Microbe 15, 177\u2013189 (2014).","journal-title":"Cell Host Microbe"},{"key":"e_1_3_4_50_2","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1002\/pmic.200600784","article-title":"myProMS, a web server for management and validation of mass spectrometry-based proteomic data","volume":"7","author":"Poullet P.","year":"2007","unstructured":"P. Poullet, S. Carpentier, E. Barillot, myProMS, a web server for management and validation of mass spectrometry-based proteomic data. Proteomics 7, 2553\u20132556 (2007).","journal-title":"Proteomics"},{"key":"e_1_3_4_51_2","doi-asserted-by":"crossref","first-page":"3572","DOI":"10.1002\/pmic.201100120","article-title":"MassChroQ: A versatile tool for mass spectrometry quantification","volume":"11","author":"Valot B.","year":"2011","unstructured":"B. Valot, O. Langella, E. Nano, M. Zivy, MassChroQ: A versatile tool for mass spectrometry quantification. Proteomics 11, 3572\u20133577 (2011).","journal-title":"Proteomics"},{"key":"e_1_3_4_52_2","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"Limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie M. E.","year":"2015","unstructured":"M. E. Ritchie ., Limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43, e47 (2015).","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_4_53_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: A practical and powerful approach to multiple testing","volume":"57","author":"Benjamini Y.","year":"1995","unstructured":"Y. Benjamini, Y. Hochberg, Controlling the false discovery rate: A practical and powerful approach to multiple testing. J. R. Stat. Soc. B 57, 289\u2013300 (1995).","journal-title":"J. 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U.S.A."},{"key":"e_1_3_4_56_2","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nmeth.1701","article-title":"SignalP 4.0: Discriminating signal peptides from transmembrane regions","volume":"8","author":"Petersen T. N.","year":"2011","unstructured":"T. N. Petersen, S. Brunak, G. von Heijne, H. Nielsen, SignalP 4.0: Discriminating signal peptides from transmembrane regions. Nat. Methods 8, 785\u2013786 (2011).","journal-title":"Nat. Methods"},{"key":"e_1_3_4_57_2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1093\/protein\/gzh037","article-title":"Feature-based prediction of non-classical and leaderless protein secretion","volume":"17","author":"Bendtsen J. D.","year":"2004","unstructured":"J. D. Bendtsen, L. J. Jensen, N. Blom, G. Von Heijne, S. Brunak, Feature-based prediction of non-classical and leaderless protein secretion. Protein Eng. Des. Sel. 17, 349\u2013356 (2004).","journal-title":"Protein Eng. Des. Sel."},{"key":"e_1_3_4_58_2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene ontology: Tool for the unification of biology","volume":"25","author":"Ashburner M.","year":"2000","unstructured":"M. Ashburner .; The Gene Ontology Consortium, Gene ontology: Tool for the unification of biology. Nat. Genet. 25, 25\u201329 (2000).","journal-title":"Nat. Genet."},{"key":"e_1_3_4_59_2","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1101\/gr.772403","article-title":"PANTHER: A library of protein families and subfamilies indexed by function","volume":"13","author":"Thomas P. D.","year":"2003","unstructured":"P. D. Thomas ., PANTHER: A library of protein families and subfamilies indexed by function. 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