{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:21:02Z","timestamp":1792110062324,"version":"4.4.0"},"reference-count":75,"publisher":"National Academy of Sciences","issue":"28","license":[{"start":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T00:00:00Z","timestamp":1576800000000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-11-BSV7-0023"],"award-info":[{"award-number":["ANR-11-BSV7-0023"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-14-CE19-0023"],"award-info":[{"award-number":["ANR-14-CE19-0023"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-16-CE32-0008-01"],"award-info":[{"award-number":["ANR-16-CE32-0008-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201010\u2010LABX\u201041"],"award-info":[{"award-number":["ANR\u201010\u2010LABX\u201041"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007601","name":"EC | Horizon 2020","doi-asserted-by":"publisher","award":["678589"],"award-info":[{"award-number":["678589"]}],"id":[{"id":"10.13039\/501100007601","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"MINEDUC | Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["2016-72170430"],"award-info":[{"award-number":["2016-72170430"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Spanish Ministry of Science and Education","award":["FPU13-0466"],"award-info":[{"award-number":["FPU13-0466"]}]},{"name":"CEI-BioTic","award":["CEI 1-2015"],"award-info":[{"award-number":["CEI 1-2015"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,7,9]]},"abstract":"<jats:p>\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species cause infectious diseases in humans and animals, but they can also live as commensals within their host tissues. How\n                    <jats:italic>Vibrio<\/jats:italic>\n                    subverts the host defenses to mount a successful infection remains poorly understood, and this knowledge is critical for predicting and managing disease. Here, we have investigated the cellular and molecular mechanisms underpinning infection and colonization of 2 virulent\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species in an ecologically relevant host model, oyster, to study interactions with marine\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species. All\n                    <jats:italic>Vibrio<\/jats:italic>\n                    strains were recognized by the immune system, but only nonvirulent strains were controlled. We showed that virulent strains were cytotoxic to hemocytes, oyster immune cells. By analyzing host and bacterial transcriptional responses to infection, together with\n                    <jats:italic>Vibrio<\/jats:italic>\n                    gene knock-outs, we discovered that\n                    <jats:italic>Vibrio crassostreae<\/jats:italic>\n                    and\n                    <jats:italic>Vibrio tasmaniensis<\/jats:italic>\n                    use distinct mechanisms to cause hemocyte lysis. Whereas\n                    <jats:italic>V. crassostreae<\/jats:italic>\n                    cytotoxicity is dependent on a direct contact with hemocytes and requires an ancestral gene encoding a protein of unknown function,\n                    <jats:italic>r5.7<\/jats:italic>\n                    ,\n                    <jats:italic>V. tasmaniensis<\/jats:italic>\n                    cytotoxicity is dependent on phagocytosis and requires intracellular secretion of T6SS effectors. We conclude that proliferation of commensal vibrios is controlled by the host immune system, preventing systemic infections in oysters, whereas the successful infection of virulent strains relies on\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species-specific molecular determinants that converge to compromise host immune cell function, allowing evasion of the host immune system.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1905747116","type":"journal-article","created":{"date-parts":[[2019,6,20]],"date-time":"2019-06-20T20:06:19Z","timestamp":1561061179000},"page":"14238-14247","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":90,"title":["Species-specific mechanisms of cytotoxicity toward immune cells determine the successful outcome of\n                    <i>Vibrio<\/i>\n                    infections"],"prefix":"10.1073","volume":"116","author":[{"given":"Tristan","family":"Rubio","sequence":"first","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Oyanedel","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5265-4669","authenticated-orcid":false,"given":"Yannick","family":"Labreuche","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Physiologie Fonctionnelle des Organismes Marins, Ifremer, F-29280 Plouzan\u00e9, France;"},{"name":"Station Biologique de Roscoff, Integrative Biology of Marine Models, UMR 8227, CNRS, Universit\u00e9 Pierre et Marie Curie Paris 06, Sorbonne Universit\u00e9s, 29688 Roscoff, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2049-2279","authenticated-orcid":false,"given":"Eve","family":"Toulza","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell, Commissariat \u00e0 l\u2019\u00c9nergie Atomique et aux \u00c9nergies Alternatives, CNRS, University Paris\u2010Sud, Universit\u00e9 Paris\u2013Saclay, 91198 Gif\u2010sur\u2010Yvette, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Bruto","sequence":"additional","affiliation":[{"name":"Station Biologique de Roscoff, Integrative Biology of Marine Models, UMR 8227, CNRS, Universit\u00e9 Pierre et Marie Curie Paris 06, Sorbonne Universit\u00e9s, 29688 Roscoff, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5162-349X","authenticated-orcid":false,"given":"Cristian","family":"Chaparro","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0609-9123","authenticated-orcid":false,"given":"Marta","family":"Torres","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"},{"name":"Institute for Integrative Biology of the Cell, Commissariat \u00e0 l\u2019\u00c9nergie Atomique et aux \u00c9nergies Alternatives, CNRS, University Paris\u2010Sud, Universit\u00e9 Paris\u2013Saclay, 91198 Gif\u2010sur\u2010Yvette, France;"},{"name":"Department of Microbiology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"de Lorgeril","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Haffner","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremie","family":"Vidal-Dupiol","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7828-0300","authenticated-orcid":false,"given":"Arnaud","family":"Lagorce","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4169-2811","authenticated-orcid":false,"given":"Bruno","family":"Petton","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Physiologie Fonctionnelle des Organismes Marins, Ifremer, F-29280 Plouzan\u00e9, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1188-1467","authenticated-orcid":false,"given":"Guillaume","family":"Mitta","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8526-5942","authenticated-orcid":false,"given":"Annick","family":"Jacq","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell, Commissariat \u00e0 l\u2019\u00c9nergie Atomique et aux \u00c9nergies Alternatives, CNRS, University Paris\u2010Sud, Universit\u00e9 Paris\u2013Saclay, 91198 Gif\u2010sur\u2010Yvette, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9rique","family":"Le Roux","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Physiologie Fonctionnelle des Organismes Marins, Ifremer, F-29280 Plouzan\u00e9, France;"},{"name":"Station Biologique de Roscoff, Integrative Biology of Marine Models, UMR 8227, CNRS, Universit\u00e9 Pierre et Marie Curie Paris 06, Sorbonne Universit\u00e9s, 29688 Roscoff, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4796-1488","authenticated-orcid":false,"given":"Guillaume M.","family":"Charri\u00e8re","sequence":"additional","affiliation":[{"name":"Interactions H\u00f4tes Pathog\u00e8nes Environnements (IHPE), University Montpellier, CNRS, Ifremer, University Perpignan Via Domitia, F-34090 Montpellier, 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Cell Host Microbe 14, 183\u2013194 (2013).","journal-title":"Cell Host Microbe"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"e1001783","DOI":"10.1371\/journal.pbio.1001783","article-title":"Divining the essence of symbiosis: Insights from the squid-vibrio model","volume":"12","author":"McFall-Ngai M.","year":"2014","unstructured":"M. McFall-Ngai, Divining the essence of symbiosis: Insights from the squid-vibrio model. PLoS Biol. 12, e1001783 (2014).","journal-title":"PLoS Biol."},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1515\/REVEH.1997.12.2.63","article-title":"Pathogenic vibrios in the natural aquatic environment","volume":"12","author":"Chakraborty S.","year":"1997","unstructured":"S. Chakraborty, G. B. Nair, S. Shinoda, Pathogenic vibrios in the natural aquatic environment. Rev. Environ. Health 12, 63\u201380 (1997).","journal-title":"Rev. Environ. Health"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"762","DOI":"10.3389\/fmicb.2017.00762","article-title":"New insights into pathogenic vibrios affecting bivalves in hatcheries: Present and future prospects","volume":"8","author":"Dubert J.","year":"2017","unstructured":"J. Dubert, J. L. Barja, J. L. Romalde, New insights into pathogenic vibrios affecting bivalves in hatcheries: Present and future prospects. Front. Microbiol. 8, 762 (2017).","journal-title":"Front. Microbiol."},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1146\/annurev-micro-090817-062148","article-title":"Eco-evolutionary dynamics linked to horizontal gene transfer in vibrios","volume":"72","author":"Le Roux F.","year":"2018","unstructured":"F. Le Roux, M. Blokesch, Eco-evolutionary dynamics linked to horizontal gene transfer in vibrios. Annu. Rev. Microbiol. 72, 89\u2013110 (2018).","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1128\/MMBR.68.3.403-431.2004","article-title":"Biodiversity of vibrios","volume":"68","author":"Thompson F. L.","year":"2004","unstructured":"F. L. Thompson, T. Iida, J. Swings, Biodiversity of vibrios. Microbiol. Mol. Biol. Rev. 68, 403\u2013431 (2004).","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.tim.2016.03.006","article-title":"Oysters and vibrios as a model for disease dynamics in wild animals","volume":"24","author":"Le Roux F.","year":"2016","unstructured":"F. Le Roux, K. M. Wegner, M. F. Polz, Oysters and vibrios as a model for disease dynamics in wild animals. Trends Microbiol. 24, 568\u2013580 (2016).","journal-title":"Trends Microbiol."},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3389\/fmicb.2012.00160","article-title":"The antimicrobial defense of the pacific oyster, Crassostrea gigas. How diversity may compensate for scarcity in the regulation of resident\/pathogenic microflora","volume":"3","author":"Schmitt P.","year":"2012","unstructured":"P. Schmitt ., The antimicrobial defense of the pacific oyster, Crassostrea gigas. How diversity may compensate for scarcity in the regulation of resident\/pathogenic microflora. Front. Microbiol. 3, 160 (2012).","journal-title":"Front. 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