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Here we have generated a\n                    <jats:italic>piggyBac<\/jats:italic>\n                    (\n                    <jats:italic>PB<\/jats:italic>\n                    ) transposon-based first-generation (F1) dominant screening system in mice, which provides unprecedented opportunities to conduct a highly efficient and affordable genome-wide phenotypic screen for an individual investigator in a single laboratory. Using this system, we carried out an F1 dominant screen for growth retardation and discovered 5 isolated mutants that carry transposon insertions hitting the genes\n                    <jats:italic>Rin2<\/jats:italic>\n                    ,\n                    <jats:italic>Rbm39<\/jats:italic>\n                    ,\n                    <jats:italic>Mll<\/jats:italic>\n                    ,\n                    <jats:italic>Zeb2<\/jats:italic>\n                    , and\n                    <jats:italic>Six1\/4<\/jats:italic>\n                    . The\n                    <jats:italic>Six1\/4<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>PB\/+<\/jats:italic>\n                    <\/jats:sup>\n                    mutant animals exhibit abnormalities in nipple recognition and milk ingestion during the breastfeeding period and also exhibit cranial nerve defects.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1906354116","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T20:55:33Z","timestamp":1566852933000},"page":"18507-18516","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":14,"title":["Efficient genome-wide first-generation phenotypic screening system in mice using the\n                    <i>piggyBac<\/i>\n                    transposon"],"prefix":"10.1073","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7037-2406","authenticated-orcid":false,"given":"Hao","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Genetics, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536;"},{"name":"Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06536;"},{"name":"School of Life Sciences, Westlake Institute for Advanced Study, Westlake University, 310024 Hangzhou, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukun","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Genetics, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536;"},{"name":"Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 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