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Here, we employed the prototypic Holliday junction-specific (HJ) DNA-binding protein RuvA and demonstrated that eDNA within biofilms formed by 3 human pathogens, uropathogenic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , nontypeable\n                    <jats:italic>Haemophilus influenzae<\/jats:italic>\n                    , and\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    was structurally related to HJ recombination intermediates. We further demonstrated that this HJ-like structure was critical to the structural and mechanical integrity of the bacterial biofilm matrix.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1909017116","type":"journal-article","created":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T20:27:41Z","timestamp":1574713661000},"page":"25068-25077","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":136,"title":["The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates"],"prefix":"10.1073","volume":"116","author":[{"given":"Aishwarya","family":"Devaraj","sequence":"first","affiliation":[{"name":"Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital, Columbus, OH 43205;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Buzzo","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital, Columbus, OH 43205;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Mashburn-Warren","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital, Columbus, OH 43205;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8895-3444","authenticated-orcid":false,"given":"Erin S.","family":"Gloag","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Ohio State University, Columbus, OH 43210;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Novotny","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital, Columbus, OH 43205;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Stoodley","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Ohio State University, Columbus, OH 43210;"},{"name":"Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH 43210;"},{"name":"National Centre for Advanced Tribology at Southampton, University of Southampton, Southampton SO17 1BJ, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren O.","family":"Bakaletz","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital, Columbus, OH 43205;"},{"name":"Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, Ohio 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D.","family":"Goodman","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Abigail Wexner Research Institute at Nationwide Children\u2019s Hospital, Columbus, OH 43205;"},{"name":"Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, Ohio 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2019,11,25]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2415"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuv015"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01935-06"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1705508114"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067629"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/lary.25826"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2012.10.011"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2011.27"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12155"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.563"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12994"},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1111\/mmi.12735","article-title":"Evaluation of the kinetics and mechanism of action of anti-integration host factor-mediated disruption of bacterial biofilms","volume":"93","author":"Brockson M. 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