{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:30:34Z","timestamp":1791145834517,"version":"4.1.0"},"reference-count":17,"publisher":"National Academy of Sciences","issue":"20","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,9,25]]},"abstract":"<jats:p>\n                    We previously reported the construction of a P1-derived artificial\n chromosome (PAC) contig encompassing a set of homozygous deletions of\n chromosome 16q23\u201324.1 found in primary ovarian tumor material and\n several tumor cell lines. Using these PAC clones in a cDNA selection\n experiment, we have isolated a\n                    <jats:italic>Sau<\/jats:italic>\n                    3A fragment homologous\n to the\n                    <jats:italic>WWOX<\/jats:italic>\n                    transcript (GenBank accession no.\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"gen\" xlink:href=\"AF211943\">AF211943<\/jats:ext-link>\n                    )\n from normal human ovarian surface epithelial (HOSE) cells. We\n demonstrate the homozygous deletion of\n                    <jats:italic>WWOX<\/jats:italic>\n                    exons from\n ovarian cancer cells and three different tumor cell lines. We also\n identify an internally deleted\n                    <jats:italic>WWOX<\/jats:italic>\n                    transcript from a\n further primary ovarian tumor. In three of these samples the deletions\n result in frameshifts, and in each case the resulting\n                    <jats:italic>WWOX<\/jats:italic>\n                    transcripts lack part, or all, of the short chain\n dehydrogenase domain and the putative mitochondrial localization\n signal. Sequencing revealed several missense polymorphisms in tumor\n cell lines and identified a high level of single nucleotide\n polymorphism (SNP) within the\n                    <jats:italic>WWOX<\/jats:italic>\n                    gene. This evidence\n strengthens the case for\n                    <jats:italic>WWOX<\/jats:italic>\n                    as a tumor suppressor gene\n in ovarian cancer and other tumor types.\n                  <\/jats:p>","DOI":"10.1073\/pnas.191175898","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:37:36Z","timestamp":1027679856000},"page":"11417-11422","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":178,"title":["<i>WWOX<\/i>\n                    : A candidate tumor suppressor gene involved in multiple tumor types"],"prefix":"10.1073","volume":"98","author":[{"given":"Adam J. W.","family":"Paige","sequence":"first","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen J.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Taylor","sequence":"additional","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen G.","family":"Hillier","sequence":"additional","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Farrington","sequence":"additional","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Scott","sequence":"additional","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Porteous","sequence":"additional","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F.","family":"Smyth","sequence":"additional","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hani","family":"Gabra","sequence":"additional","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E. Vivienne","family":"Watson","sequence":"additional","affiliation":[{"name":"Imperial Cancer Research Fund (ICRF) Medical Oncology Unit,\r Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United\r Kingdom; ICRF Mutation Detection Facility, St. James\r University Hospital, Beckett Street, Leeds LS9 7TF, United Kingdom;\r University of Edinburgh Centre for Reproductive\r Biology, 37 Chalmers Street, Edinburgh EH3 9ET, United Kingdom;\r Medical Research Council Human Genetics Unit, Western\r General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom;\r Medical Genetics..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2001,9,25]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"5605","volume":"56","author":"Chen T","year":"1996","unstructured":"T Chen, A Sahin, C M Aldaz Cancer Res 56, 5605\u20135609 (1996).","journal-title":"Cancer Res"},{"key":"e_1_3_4_2_2","first-page":"3645","volume":"60","author":"Paris P L","year":"2000","unstructured":"P L Paris, J S Witte, P A Kupelian, H Levin, E A Klein, W J Catalona, G Casey Cancer Res 60, 3645\u20133649 (2000).","journal-title":"Cancer Res"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1038\/sj.bjc.6690431","volume":"80","author":"Piao Z","year":"1999","unstructured":"Z Piao, C Park, J J Kim, H Kim Br J Cancer 80, 850\u2013854 (1999).","journal-title":"Br J Cancer"},{"key":"e_1_3_4_4_2","first-page":"1690","volume":"60","author":"Paige A J W","year":"2000","unstructured":"A J W Paige, K J Taylor, A Stewart, J G Sgouros, H Gabra, G C Sellar, J F Smyth, D J Porteous, J E V Watson Cancer Res 60, 1690\u20131697 (2000).","journal-title":"Cancer Res"},{"key":"e_1_3_4_5_2","first-page":"1683","volume":"60","author":"Mangelsdorf M","year":"2000","unstructured":"M Mangelsdorf, K Ried, E Woollatt, S Dayan, H Eyre, M Finnis, L Hobson, J Nancarrow, D Venter, E Baker, R I Richards Cancer Res 60, 1683\u20131689 (2000).","journal-title":"Cancer Res"},{"key":"e_1_3_4_6_2","first-page":"2140","volume":"60","author":"Bednarek A K","year":"2000","unstructured":"A K Bednarek, K J Laflin, R L Daniel, Q Liao, K A Hawkins, C M Aldaz Cancer Res 60, 2140\u20132145 (2000).","journal-title":"Cancer Res"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1093\/hmg\/9.11.1651","volume":"9","author":"Ried K","year":"2000","unstructured":"K Ried, M Finnis, L Hobson, M Mangelsdorf, S Dayan, J K Nancarrow, E Woollatt, G Kremmidiotis, A Gardner, D Venter, et al. 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