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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,10,15]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    [FeFe]-hydrogenases catalyze H\n                    <jats:sub>2<\/jats:sub>\n                    evolution at extraordinary rates at low overpotential using base metal (not platinum). In the field of renewable energy, the biosynthesis of these enzymes\u2019 active sites, the H-cluster, is of intense interest. Among its many cofactors, the azadithiolate is unique, and its biosynthesis remains enigmatic. Three Fe\u2013S proteins, HydE, HydF, and HydG, are essential for the H-cluster bioassembly. This paper describes an in vitro assembly approach where a synthetic [Fe(cysteine)(CO)\n                    <jats:sub>2<\/jats:sub>\n                    (CN)] complex, \u201csyn-B,\u201d allows HydG-free biosynthesis of the active enzyme. Using isotopic and S\/Se labeling, syn-B is shown to contribute Fe(CO)\n                    <jats:sub>2<\/jats:sub>\n                    (CN)S to the biosynthesis. This work sets the stage for further investigation of the H-cluster bioassembly.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1913324116","type":"journal-article","created":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T22:26:14Z","timestamp":1569882374000},"page":"20850-20855","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":45,"title":["The binuclear cluster of [FeFe] hydrogenase is formed with sulfur donated by cysteine of an [Fe(Cys)(CO)\n                    <sub>2<\/sub>\n                    (CN)] organometallic precursor"],"prefix":"10.1073","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8043-3436","authenticated-orcid":false,"given":"Guodong","family":"Rao","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of California, Davis, CA 95616;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Pattenaude","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences, University of Illinois at Urbana\u2013Champaign, Urbana, IL 61801;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Alwan","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, Oregon Health and Science University, Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9755-7838","authenticated-orcid":false,"given":"Ninian J.","family":"Blackburn","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, Oregon Health and Science University, Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0889-8436","authenticated-orcid":false,"given":"R. 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