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However, the rules governing the distribution of IEG expression within neuronal assemblies have not been comprehensively studied. To this end, we applied single-molecule fluorescence in situ hybridization (smFISH) on striatal tissue following cocaine experience and analyzed a published dataset of single-cell RNA sequencing from cortical tissue following light exposure. We report principles governing IEG induction and identify region-specific rules defining cell-specific recruitment. Our results suggest the existence of \u201csuperensembles\u201d of neurons found in spatially defined clusters and characterized by the coherent and robust expression of multiple IEGs.<\/jats:p>","DOI":"10.1073\/pnas.1913658116","type":"journal-article","created":{"date-parts":[[2019,10,21]],"date-time":"2019-10-21T20:39:39Z","timestamp":1571690379000},"page":"23304-23310","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":33,"title":["Subregion-specific rules govern the distribution of neuronal immediate-early gene induction"],"prefix":"10.1073","volume":"117","author":[{"given":"Ben Jerry","family":"Gonzales","sequence":"first","affiliation":[{"name":"Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. 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