{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T10:17:32Z","timestamp":1789208252898,"version":"build-2803163510"},"reference-count":23,"publisher":"National Academy of Sciences","issue":"20","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,9,25]]},"abstract":"<jats:p>\n                    We report that complete protection against malaria and total inhibition of liver stage development and parasitemia was obtained in 100% of BALB\/c mice primed with a replication-defective recombinant adenovirus expressing the circumsporozoite (CS) protein of\n                    <jats:italic>Plasmodium yoelii<\/jats:italic>\n                    (AdPyCS), followed by a booster with an attenuated recombinant vaccinia virus, expressing the same malaria antigen, VacPyCS. We found increased levels of activated CS-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    and CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, higher anti-sporozoite antibody titers, and greater protection in these mice, when the time between priming and boosting with these two viral vectors was extended from 2 to 8 or more weeks. Most importantly, by using this immunization regimen, the protection of the immunized mice was found to be long-lasting, namely complete resistance to infection of all animals 3 1\/2 months after priming. These results indicate that immunization with AdPyCS generates highly effective memory T and B cells that can be recalled long after priming by boosting with VacPyCS.\n                  <\/jats:p>","DOI":"10.1073\/pnas.191380898","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:34:10Z","timestamp":1027679650000},"page":"11491-11496","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":101,"title":["Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen"],"prefix":"10.1073","volume":"98","author":[{"given":"Oscar","family":"Bru\u00f1a-Romero","sequence":"first","affiliation":[{"name":"Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Gonz\u00e1lez-Aseguinolaza","sequence":"additional","affiliation":[{"name":"Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julius C. R.","family":"Hafalla","sequence":"additional","affiliation":[{"name":"Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moriya","family":"Tsuji","sequence":"additional","affiliation":[{"name":"Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth S.","family":"Nussenzweig","sequence":"additional","affiliation":[{"name":"Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2001,9,11]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"1","volume":"80","author":"Rodrigues M","year":"1993","unstructured":"M Rodrigues, R S Nussenzweig, F Zavala Immunology 80, 1\u20135 (1993).","journal-title":"Immunology"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60695-1"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.8.2529-2534.1991"},{"key":"e_1_3_3_4_2","first-page":"493","volume":"144","author":"Nussenzweig V","year":"1989","unstructured":"V Nussenzweig, R S Nussenzweig Bull Mem Acad R Med Belg 144, 493\u2013504 (1989).","journal-title":"Bull Mem Acad R Med Belg"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.4049\/jimmunol.158.3.1268","volume":"158","author":"Rodrigues E G","year":"1997","unstructured":"E G Rodrigues, F Zavala, D Eichinger, J M Wilson, M Tsuji J Immunol 158, 1268\u20131274 (1997).","journal-title":"J Immunol"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.11.5214"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.13.7648"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.4.2084"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00344-8"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/3.6.579"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.10.4851-4857.1990"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(96)02574-1"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)00327-S"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.0108"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830200604"},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"4636","DOI":"10.4049\/jimmunol.153.10.4636","volume":"153","author":"Rodrigues M","year":"1994","unstructured":"M Rodrigues, S Li, K Murata, D Rodriguez, J R Rodriguez, I Bacik, J R Bennink, J W Yewdell, A Garcia-Sastre, R S Nussenzweig, et al. J Immunol 153, 4636\u20134648 (1994).","journal-title":"J Immunol"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.221"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.8.6907-6910.1998"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.5.2338"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00096-6"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0738"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1996.0255"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00107-7"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.191380898","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,22]],"date-time":"2023-04-22T08:12:43Z","timestamp":1682151163000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.191380898"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9,11]]},"references-count":23,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2001,9,25]]}},"alternative-id":["10.1073\/pnas.191380898"],"URL":"https:\/\/doi.org\/10.1073\/pnas.191380898","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,9,11]]},"assertion":[{"value":"2001-05-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-07-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-09-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}