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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,12,26]]},"abstract":"<jats:p>\n                    Foxp3\n                    <jats:sup>+<\/jats:sup>\n                    CD4\n                    <jats:sup>+<\/jats:sup>\n                    regulatory T cells (Tregs) play important roles in controlling both homeostatic processes and immune responses at the tissue and organismal levels. For example, Tregs promote muscle regeneration in acute or chronic injury models by direct effects on local muscle progenitor cells, as well as on infiltrating inflammatory cells. Muscle Tregs have a transcriptome, a T cell receptor (TCR) repertoire, and effector capabilities distinct from those of classical, lymphoid-organ Tregs, but it has proven difficult to study the provenance and functions of these unique features due to the rarity of muscle Tregs and their fragility on isolation. Here, we attempted to sidestep these hindrances by generating, characterizing, and employing a line of mice carrying rearranged transgenes encoding the TCR\u03b1 and TCR\u03b2 chains from a Treg clone rapidly and specifically expanded within acutely injured hindlimb muscle of young mice. Tregs displaying the transgene-encoded TCR preferentially accumulated in injured hindlimb muscle in a TCR-dependent manner both in the straight transgenic model and in adoptive-transfer systems; non-Treg CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells expressing the same TCR did not specifically localize in injured muscle. The definitive muscle-Treg transcriptome was not established until the transgenic Tregs inhabited muscle. When crossed onto the\n                    <jats:italic>mdx<\/jats:italic>\n                    model of Duchenne muscular dystrophy, the muscle-Treg TCR transgenes drove enhanced accumulation of Tregs in hindlimb muscles and improved muscle regeneration. These findings invoke the possibility of harnessing muscle Tregs or their TCRs for treatment of skeletal muscle pathologies.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1914848116","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T17:45:49Z","timestamp":1575999949000},"page":"26727-26733","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":65,"title":["T cell receptor specificity drives accumulation of a reparative population of regulatory T cells within acutely injured skeletal muscle"],"prefix":"10.1073","volume":"116","author":[{"given":"Jun","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115;"},{"name":"Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women\u2019s Hospital, Boston, MA 02115;"},{"name":"Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, 61005 Gwangju, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilson","family":"Kuswanto","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115;"},{"name":"Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women\u2019s Hospital, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Benoist","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115;"},{"name":"Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women\u2019s Hospital, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Mathis","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115;"},{"name":"Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women\u2019s Hospital, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2019,12,10]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1038\/nature10772","article-title":"Extrathymically generated regulatory T cells control mucosal TH2 inflammation","volume":"482","author":"Josefowicz S. 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