{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:56:34Z","timestamp":1792032994095,"version":"4.4.0"},"reference-count":38,"publisher":"National Academy of Sciences","issue":"24","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-13-IFEC-0003"],"award-info":[{"award-number":["ANR-13-IFEC-0003"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR; ANR-14-CE14-0012-01"],"award-info":[{"award-number":["ANR; ANR-14-CE14-0012-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-INBS-04"],"award-info":[{"award-number":["ANR-10-INBS-04"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-12-01"],"award-info":[{"award-number":["ANR-10-LABX-12-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,6,16]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Coxiella<\/jats:italic>\n                    <jats:italic>burnetii<\/jats:italic>\n                    is a stealth pathogen that evades innate immune recognition by inhibiting the NF-\u03baB signaling pathway. This process is mediated by the bacterial Dot\/Icm secretion system; however, the bacterial effector\/s, as well as the molecular mechanism involved in this process have remained unknown to date. Here, by investigating\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    proteins with eukaryotic-like features (EUGENs), we discovered a new effector protein, NopA (nucleolar protein A), which localizes at nucleoli of infected cells and perturbs nucleocytoplasmic transport by manipulating the intracellular gradients of the GTPase Ran. In doing so, NopA reduces the nuclear levels of transcription factors involved in the innate immune sensing of pathogens and single-handedly down-modulates the expression of a panel of cytokines.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1914892117","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T20:29:44Z","timestamp":1591043384000},"page":"13708-13718","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":51,"title":["Modulation of innate immune signaling by a\n                    <i>Coxiella burnetii<\/i>\n                    eukaryotic-like effector protein"],"prefix":"10.1073","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5751-2208","authenticated-orcid":false,"given":"Melanie","family":"Burette","sequence":"first","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004, CNRS, Universit\u00e9 de Montpellier, 34293 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Allombert","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004, CNRS, Universit\u00e9 de Montpellier, 34293 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5558-6860","authenticated-orcid":false,"given":"Karine","family":"Lambou","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004, CNRS, Universit\u00e9 de Montpellier, 34293 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghizlane","family":"Maarifi","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004, CNRS, Universit\u00e9 de Montpellier, 34293 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9793-419X","authenticated-orcid":false,"given":"S\u00e9bastien","family":"Nisole","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004, CNRS, Universit\u00e9 de Montpellier, 34293 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9129-3894","authenticated-orcid":false,"given":"Elizabeth","family":"Di Russo Case","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Pathogenesis, Texas A&amp;M Health Science Center College of Medicine, Bryan, TX 77807\u20133260;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3168-0697","authenticated-orcid":false,"given":"Fabien P.","family":"Blanchet","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004, CNRS, Universit\u00e9 de Montpellier, 34293 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7715-7167","authenticated-orcid":false,"given":"Cedric","family":"Hassen-Khodja","sequence":"additional","affiliation":[{"name":"Montpellier Ressources Imagerie (MRI), BioCampus Montpellier, CNRS, INSERM, Universit\u00e9 de Montpellier, 34293 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8406-9421","authenticated-orcid":false,"given":"St\u00e9phanie","family":"Cabantous","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Canc\u00e9rologie de Toulouse, INSERM, Universit\u00e9 Paul Sabatier-Toulouse III, CNRS, 31037 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3896-8581","authenticated-orcid":false,"given":"James","family":"Samuel","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Pathogenesis, Texas A&amp;M Health Science Center College of Medicine, Bryan, TX 77807\u20133260;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8568-3725","authenticated-orcid":false,"given":"Eric","family":"Martinez","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004, CNRS, Universit\u00e9 de Montpellier, 34293 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5499-8759","authenticated-orcid":false,"given":"Matteo","family":"Bonazzi","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004, CNRS, Universit\u00e9 de Montpellier, 34293 Montpellier, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2020,6]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2539"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12445"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2114"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005653"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007023"},{"key":"e_1_3_3_6_2","first-page":"243","article-title":"Beginning to understand the role of the Type IV secretion system effector proteins in Coxiella burnetii pathogenesis","volume":"413","author":"L\u00fchrmann A.","year":"2017","unstructured":"A. L\u00fchrmann, H. J. Newton, M. Bonazzi, Beginning to understand the role of the Type IV secretion system effector proteins in Coxiella burnetii pathogenesis. Curr. Top. Microbiol. Immunol. 413, 243\u2013268 (2017).","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2016-0044"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10205"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00188"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004013"},{"key":"e_1_3_3_11_2","first-page":"e52851","article-title":"Generation and multi-phenotypic high-content screening of Coxiella burnetii transposon mutants","volume":"2015","author":"Martinez E.","year":"2015","unstructured":"E. Martinez, F. Cantet, M. Bonazzi, Generation and multi-phenotypic high-content screening of Coxiella burnetii transposon mutants. J. Vis. Exp. 2015, e52851 (2015).","journal-title":"J. Vis. 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