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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2019,12,26]]},"abstract":"<jats:p>\n                    A complete hydatidiform mole (CHM) is androgenetic in origin and characterized by enhanced trophoblastic proliferation and the absence of fetal tissue. In 15 to 20% of cases, CHMs are followed by malignant gestational trophoblastic neoplasms including choriocarcinoma. Aberrant genomic imprinting may be responsible for trophoblast hypertrophy in CHMs, but the detailed mechanisms are still elusive, partly due to the lack of suitable animal or in vitro models. We recently developed a culture system of human trophoblast stem (TS) cells. In this study, we apply this system to CHMs for a better understanding of their molecular pathology. CHM-derived TS cells, designated as TS\n                    <jats:sup>mole<\/jats:sup>\n                    cells, are morphologically similar to biparental TS (TS\n                    <jats:sup>bip<\/jats:sup>\n                    ) cells and express TS-specific markers such as GATA3, KRT7, and TFAP2C. Interestingly, TS\n                    <jats:sup>mole<\/jats:sup>\n                    cells have a growth advantage over TS\n                    <jats:sup>bip<\/jats:sup>\n                    cells only after they reach confluence. We found that p57\n                    <jats:sup>KIP2<\/jats:sup>\n                    , a maternally expressed gene encoding a cyclin-dependent kinase inhibitor, is strongly induced by increased cell density in TS\n                    <jats:sup>bip<\/jats:sup>\n                    cells, but not in TS\n                    <jats:sup>mole<\/jats:sup>\n                    cells. Knockout and overexpression studies suggest that loss of p57\n                    <jats:sup>KIP2<\/jats:sup>\n                    expression would be the major cause of the reduced sensitivity to contact inhibition in CHMs. Our findings shed light on the molecular mechanism underlying the pathogenesis of CHMs and could have broad implications in tumorigenesis beyond CHMs because silencing of\n                    <jats:italic>p57<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>KIP2<\/jats:italic>\n                    <\/jats:sup>\n                    is frequently observed in a variety of human tumors.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1916019116","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T20:25:57Z","timestamp":1575318357000},"page":"26606-26613","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":41,"title":["Loss of p57\n                    <sup>KIP2<\/sup>\n                    expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells"],"prefix":"10.1073","volume":"116","author":[{"given":"Sota","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine, Aoba-ku, 980-8575 Sendai, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Okae","sequence":"additional","affiliation":[{"name":"Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine, Aoba-ku, 980-8575 Sendai, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine, Aoba-ku, 980-8575 Sendai, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akane","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine, Aoba-ku, 980-8575 Sendai, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Kumada","sequence":"additional","affiliation":[{"name":"Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine, Aoba-ku, 980-8575 Sendai, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Yaegashi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Aoba-ku, 980-8575 Sendai, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Arima","sequence":"additional","affiliation":[{"name":"Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine, Aoba-ku, 980-8575 Sendai, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2019,12,2]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1080\/19336918.2015.1093275","article-title":"The hydatidiform mole","volume":"10","author":"Candelier J. 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Berkowitz, Gestational trophoblastic disease. Lancet 376, 717\u2013729 (2010).","journal-title":"Lancet"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/268633a0","article-title":"Androgenetic origin of hydatidiform mole","volume":"268","author":"Kajii T.","year":"1977","unstructured":"T. Kajii, K. Ohama, Androgenetic origin of hydatidiform mole. Nature 268, 633\u2013634 (1977).","journal-title":"Nature"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1038\/292551a0","article-title":"Dispermic origin of XY hydatidiform moles","volume":"292","author":"Ohama K.","year":"1981","unstructured":"K. Ohama ., Dispermic origin of XY hydatidiform moles. 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Deposited 24 October 2019."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1916019116","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1916019116","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T17:41:37Z","timestamp":1654623697000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1916019116"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,2]]},"references-count":38,"journal-issue":{"issue":"52","published-print":{"date-parts":[[2019,12,26]]}},"alternative-id":["10.1073\/pnas.1916019116"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1916019116","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,2]]},"assertion":[{"value":"2019-12-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}