{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:48:38Z","timestamp":1791316118725,"version":"4.3.1"},"reference-count":55,"publisher":"National Academy of Sciences","issue":"13","license":[{"start":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T00:00:00Z","timestamp":1584316800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"Department of Health | National Health and Medical Research Council","doi-asserted-by":"publisher","award":["PS2857"],"award-info":[{"award-number":["PS2857"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"Department of Health | National Health and Medical Research Council","doi-asserted-by":"publisher","award":["PS2897"],"award-info":[{"award-number":["PS2897"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012912","name":"imperial college healthcare charity","doi-asserted-by":"crossref","award":["RF16\\10001"],"award-info":[{"award-number":["RF16\\10001"]}],"id":[{"id":"10.13039\/100012912","id-type":"DOI","asserted-by":"crossref"}]},{"name":"BRC Cancer Imperial College","award":["P83204"],"award-info":[{"award-number":["P83204"]}]},{"name":"National research development and innovation office of Hungary","award":["KNN121510"],"award-info":[{"award-number":["KNN121510"]}]},{"name":"National research development and innovation office of Hungary","award":["NVKP_16-1-2016-0042"],"award-info":[{"award-number":["NVKP_16-1-2016-0042"]}]},{"name":"Waters Corporation","award":["P53626_WSSB"],"award-info":[{"award-number":["P53626_WSSB"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,3,31]]},"abstract":"<jats:p>\n                    Clearance of surgical margins in cervical cancer prevents the need for adjuvant chemoradiation and allows fertility preservation. In this study, we determined the capacity of the rapid evaporative ionization mass spectrometry (REIMS), also known as intelligent knife (iKnife), to discriminate between healthy, preinvasive, and invasive cervical tissue. Cervical tissue samples were collected from women with healthy, human papilloma virus (HPV) \u00b1 cervical intraepithelial neoplasia (CIN), or cervical cancer. A handheld diathermy device generated surgical aerosol, which was transferred into a mass spectrometer for subsequent chemical analysis. Combination of principal component and linear discriminant analysis and least absolute shrinkage and selection operator was employed to study the spectral differences between groups. Significance of discriminatory\n                    <jats:italic>m\/z<\/jats:italic>\n                    features was tested using univariate statistics and tandem MS performed to elucidate the structure of the significant peaks allowing separation of the two classes. We analyzed 87 samples (normal = 16, HPV \u00b1 CIN = 50, cancer = 21 patients). The iKnife discriminated with 100% accuracy normal (100%) vs. HPV \u00b1 CIN (100%) vs. cancer (100%) when compared to histology as the gold standard. When comparing normal vs. cancer samples, the accuracy was 100% with a sensitivity of 100% (95% CI 83.9 to 100) and specificity 100% (79.4 to 100). Univariate analysis revealed significant MS peaks in the cancer-to-normal separation belonging to various classes of complex lipids. The iKnife discriminates healthy from premalignant and invasive cervical lesions with high accuracy and can improve oncological outcomes and fertility preservation of women treated surgically for cervical cancer. Larger in vivo research cohorts are required to validate these findings.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1916960117","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T20:25:37Z","timestamp":1584390337000},"page":"7338-7346","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":101,"title":["The intelligent knife (iKnife) and its intraoperative diagnostic advantage for the treatment of cervical disease"],"prefix":"10.1073","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4733-0034","authenticated-orcid":false,"given":"Menelaos","family":"Tzafetas","sequence":"first","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Obstetrics and Gynaecology, Imperial College Healthcare NHS Trust, London W2 1NY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Mitra","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Obstetrics and Gynaecology, Imperial College Healthcare NHS Trust, London W2 1NY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Paraskevaidi","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsolt","family":"Bodai","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilkka","family":"Kalliala","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Obstetrics and Gynaecology, University Hospital Helsinki, Helsinki University, 00290 Helsinki, Finland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Bowden","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Obstetrics and Gynaecology, Imperial College Healthcare NHS Trust, London W2 1NY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantinos","family":"Lathouras","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Imperial College Healthcare NHS Trust, London W2 1NY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Rosini","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcell","family":"Szasz","sequence":"additional","affiliation":[{"name":"Department of Tumour Biology, National Koranyi Institute of Pulmonology, 1122 Budapest, Hungary;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adele","family":"Savage","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eftychios","family":"Manoli","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Imperial College London, London SW7 2DD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Balog","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Waters Research Center, 1031 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"McKenzie","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deirdre","family":"Lyons","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Imperial College Healthcare NHS Trust, London W2 1NY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip","family":"Bennett","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Obstetrics and Gynaecology, Imperial College Healthcare NHS Trust, London W2 1NY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4186-5567","authenticated-orcid":false,"given":"David","family":"MacIntyre","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadaf","family":"Ghaem-Maghami","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Obstetrics and Gynaecology, Imperial College Healthcare NHS Trust, London W2 1NY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoltan","family":"Takats","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7165-0735","authenticated-orcid":false,"given":"Maria","family":"Kyrgiou","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, London SW7 2DD, United Kingdom;"},{"name":"Department of Obstetrics and Gynaecology, Imperial College Healthcare NHS Trust, London W2 1NY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2020,3,16]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1097\/IGC.0000000000001216","article-title":"The European Society of Gynaecological Oncology\/European Society for Radiotherapy and Oncology\/European Society of Pathology guidelines for the management of patients with cervical cancer","volume":"28","author":"Cibula D.","year":"2018","unstructured":"D. Cibula ., The European Society of Gynaecological Oncology\/European Society for Radiotherapy and Oncology\/European Society of Pathology guidelines for the management of patients with cervical cancer. Int. J. Gynecol. Cancer 28, 641\u2013655 (2018).","journal-title":"Int. J. Gynecol. Cancer"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1093\/annonc\/mdm377","article-title":"Burden of cervical cancer in the 27 member states of the European Union: Estimates for 2004","volume":"18","author":"Arbyn M.","year":"2007","unstructured":"M. Arbyn, P. Autier, J. Ferlay, Burden of cervical cancer in the 27 member states of the European Union: Estimates for 2004. Ann. Oncol. 18, 1423\u20131425 (2007).","journal-title":"Ann. Oncol."},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.1002\/cncr.23756","article-title":"Burden of cervical cancer in the United States, 1998-2003","volume":"113","author":"Watson M.","year":"2008","unstructured":"M. Watson ., Burden of cervical cancer in the United States, 1998-2003. Cancer 113 (suppl. 10), 2855\u20132864 (2008).","journal-title":"Cancer"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/S1470-2045(10)70084-X","article-title":"Fertility-sparing surgery in patients with cervical cancer","volume":"12","author":"Rob L.","year":"2011","unstructured":"L. Rob, P. Skapa, H. Robova, Fertility-sparing surgery in patients with cervical cancer. Lancet Oncol. 12, 192\u2013200 (2011).","journal-title":"Lancet Oncol."},{"key":"e_1_3_4_5_2","unstructured":"Cancer Research UK Cervical cancer statistics. https:\/\/www.cancerresearchuk.org\/health-professional\/cancer-statistics\/statistics-by-cancer-type\/cervical-cancer. Accessed 22 June 2019."},{"key":"e_1_3_4_6_2","unstructured":"Office for National Statistics Births by parents\u2019 characteristics in England and Wales: 2015. https:\/\/www.ons.gov.uk\/peoplepopulationandcommunity\/birthsdeathsandmarriages\/livebirths\/bulletins\/birthsbyparentscharacteristicsinenglandandwales\/2015. Accessed 17 June 2019."},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"e34","DOI":"10.3802\/jgo.2017.28.e34","article-title":"Randomized study between radical surgery and radiotherapy for the treatment of stage IB-IIA cervical cancer: 20-year update","volume":"28","author":"Landoni F.","year":"2017","unstructured":"F. Landoni ., Randomized study between radical surgery and radiotherapy for the treatment of stage IB-IIA cervical cancer: 20-year update. J. Gynecol. Oncol. 28, e34 (2017).","journal-title":"J. Gynecol. Oncol."},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.radonc.2018.03.003","article-title":"The European Society of Gynaecological Oncology\/European Society for Radiotherapy and Oncology\/European Society of Pathology guidelines for the management of patients with cervical cancer","volume":"127","author":"Cibula D.","year":"2018","unstructured":"D. Cibula ., The European Society of Gynaecological Oncology\/European Society for Radiotherapy and Oncology\/European Society of Pathology guidelines for the management of patients with cervical cancer. Radiother. Oncol. 127, 404\u2013416 (2018).","journal-title":"Radiother. Oncol."},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S0140-6736(97)02250-2","article-title":"Randomised study of radical surgery versus radiotherapy for stage Ib-IIa cervical cancer","volume":"350","author":"Landoni F.","year":"1997","unstructured":"F. Landoni ., Randomised study of radical surgery versus radiotherapy for stage Ib-IIa cervical cancer. Lancet 350, 535\u2013540 (1997).","journal-title":"Lancet"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1056\/NEJMoa1806395","article-title":"Minimally invasive versus abdominal radical hysterectomy for cervical cancer","volume":"379","author":"Ramirez P. T.","year":"2018","unstructured":"P. T. Ramirez ., Minimally invasive versus abdominal radical hysterectomy for cervical cancer. N. Engl. J. Med. 379, 1895\u20131904 (2018).","journal-title":"N. Engl. J. Med."},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.ygyno.2013.02.001","article-title":"High false negative rate of frozen section examination of sentinel lymph nodes in patients with cervical cancer","volume":"129","author":"Slama J.","year":"2013","unstructured":"J. Slama, P. Dundr, L. Dusek, D. Cibula, High false negative rate of frozen section examination of sentinel lymph nodes in patients with cervical cancer. Gynecol. Oncol. 129, 384\u2013388 (2013).","journal-title":"Gynecol. Oncol."},{"key":"e_1_3_4_12_2","unstructured":"Scottish Intercollegiate Guidelines Network \u201cManagement of cervical cancer: A national clinical guideline\u201d (Scottish Intercollegiate Guidelines Network Edinburgh UK 2008)."},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ygyno.2012.10.028","article-title":"The impact of close surgical margins after radical hysterectomy for early-stage cervical cancer","volume":"128","author":"McCann G. A.","year":"2013","unstructured":"G. A. McCann ., The impact of close surgical margins after radical hysterectomy for early-stage cervical cancer. Gynecol. Oncol. 128, 44\u201348 (2013).","journal-title":"Gynecol. Oncol."},{"key":"e_1_3_4_14_2","doi-asserted-by":"crossref","first-page":"2778","DOI":"10.1038\/bjc.2013.656","article-title":"Comparisons of vaginal and abdominal radical trachelectomy for early-stage cervical cancer: Preliminary results of a multi-center research in China","volume":"109","author":"Cao D. Y.","year":"2013","unstructured":"D. Y. Cao .; China Gynecologic Oncology Group, Comparisons of vaginal and abdominal radical trachelectomy for early-stage cervical cancer: Preliminary results of a multi-center research in China. Br. J. Cancer 109, 2778\u20132782 (2013).","journal-title":"Br. J. Cancer"},{"key":"e_1_3_4_15_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1067\/mob.2003.124","article-title":"Radical trachelectomy and pelvic lymphadenectomy with uterine preservation in the treatment of cervical cancer","volume":"188","author":"Schlaerth J. B.","year":"2003","unstructured":"J. B. Schlaerth, N. M. Spirtos, A. C. Schlaerth, Radical trachelectomy and pelvic lymphadenectomy with uterine preservation in the treatment of cervical cancer. Am. J. Obstet. Gynecol. 188, 29\u201334 (2003).","journal-title":"Am. J. Obstet. Gynecol."},{"key":"e_1_3_4_16_2","unstructured":"European Society of Gynaecological Oncology (ESGO) Cervical cancer guidelines. https:\/\/www.esgo.org\/media\/2015\/12\/Cervix-GL-2017-Amended-brochure.pdf. Accessed 22 June 2019."},{"key":"e_1_3_4_17_2","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/j.ygyno.2012.03.001","article-title":"Indications and long-term clinical outcomes in 282 patients with pelvic exenteration for advanced or recurrent cervical cancer","volume":"125","author":"Schmidt A. M.","year":"2012","unstructured":"A. M. Schmidt, P. Imesch, D. Fink, H. Egger, Indications and long-term clinical outcomes in 282 patients with pelvic exenteration for advanced or recurrent cervical cancer. Gynecol. Oncol. 125, 604\u2013609 (2012).","journal-title":"Gynecol. Oncol."},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.ygyno.2008.05.029","article-title":"Frozen-section evaluation of cervical adenocarcinoma at time of radical trachelectomy: Pathologic pitfalls and the application of an objective scoring system","volume":"110","author":"Park K. J.","year":"2008","unstructured":"K. J. Park, R. A. Soslow, Y. Sonoda, R. R. Barakat, N. R. Abu-Rustum, Frozen-section evaluation of cervical adenocarcinoma at time of radical trachelectomy: Pathologic pitfalls and the application of an objective scoring system. Gynecol. Oncol. 110, 316\u2013323 (2008).","journal-title":"Gynecol. Oncol."},{"key":"e_1_3_4_19_2","doi-asserted-by":"crossref","first-page":"7343","DOI":"10.1021\/ac101283x","article-title":"Identification of biological tissues by rapid evaporative ionization mass spectrometry","volume":"82","author":"Balog J.","year":"2010","unstructured":"J. Balog ., Identification of biological tissues by rapid evaporative ionization mass spectrometry. Anal. Chem. 82, 7343\u20137350 (2010).","journal-title":"Anal. Chem."},{"key":"e_1_3_4_20_2","doi-asserted-by":"crossref","first-page":"194ra93","DOI":"10.1126\/scitranslmed.3005623","article-title":"Intraoperative tissue identification using rapid evaporative ionization mass spectrometry","volume":"5","author":"Balog J.","year":"2013","unstructured":"J. Balog ., Intraoperative tissue identification using rapid evaporative ionization mass spectrometry. Sci. Transl. Med. 5, 194ra93 (2013).","journal-title":"Sci. Transl. Med."},{"key":"e_1_3_4_21_2","doi-asserted-by":"crossref","first-page":"180","DOI":"10.4103\/0973-1482.19600","article-title":"Surface activity, lipid profiles and their implications in cervical cancer","volume":"1","author":"Preetha A.","year":"2005","unstructured":"A. Preetha, R. Banerjee, N. Huilgol, Surface activity, lipid profiles and their implications in cervical cancer. J. Cancer Res. Ther. 1, 180\u2013186 (2005).","journal-title":"J. Cancer Res. Ther."},{"key":"e_1_3_4_22_2","doi-asserted-by":"crossref","first-page":"6555","DOI":"10.1021\/ac501075f","article-title":"Characterization and identification of clinically relevant microorganisms using rapid evaporative ionization mass spectrometry","volume":"86","author":"Strittmatter N.","year":"2014","unstructured":"N. Strittmatter ., Characterization and identification of clinically relevant microorganisms using rapid evaporative ionization mass spectrometry. Anal. Chem. 86, 6555\u20136562 (2014).","journal-title":"Anal. Chem."},{"key":"e_1_3_4_23_2","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1007\/s00464-016-5121-5","article-title":"A novel methodology for in vivo endoscopic phenotyping of colorectal cancer based on real-time analysis of the mucosal lipidome: A prospective observational study of the iKnife","volume":"31","author":"Alexander J.","year":"2017","unstructured":"J. Alexander ., A novel methodology for in vivo endoscopic phenotyping of colorectal cancer based on real-time analysis of the mucosal lipidome: A prospective observational study of the iKnife. Surg. Endosc. 31, 1361\u20131370 (2017).","journal-title":"Surg. Endosc."},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1038\/s41416-018-0048-3","article-title":"The surgical intelligent knife distinguishes normal, borderline and malignant gynaecological tissues using rapid evaporative ionisation mass spectrometry (REIMS)","volume":"118","author":"Phelps D. L.","year":"2018","unstructured":"D. L. Phelps ., The surgical intelligent knife distinguishes normal, borderline and malignant gynaecological tissues using rapid evaporative ionisation mass spectrometry (REIMS). Br. J. Cancer 118, 1349\u20131358 (2018).","journal-title":"Br. J. Cancer"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1021\/ac102613m","article-title":"In situ, real-time identification of biological tissues by ultraviolet and infrared laser desorption ionization mass spectrometry","volume":"83","author":"S\u00e4chfer K. C.","year":"2011","unstructured":"K. C. S\u00e4chfer ., In situ, real-time identification of biological tissues by ultraviolet and infrared laser desorption ionization mass spectrometry. Anal. Chem. 83, 1632\u20131640 (2011).","journal-title":"Anal. Chem."},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s13058-017-0845-2","article-title":"Rapid evaporative ionisation mass spectrometry of electrosurgical vapours for the identification of breast pathology: Towards an intelligent knife for breast cancer surgery","volume":"19","author":"St John E. R.","year":"2017","unstructured":"E. R. St John ., Rapid evaporative ionisation mass spectrometry of electrosurgical vapours for the identification of breast pathology: Towards an intelligent knife for breast cancer surgery. Breast Cancer Res. 19, 59 (2017).","journal-title":"Breast Cancer Res."},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"7729","DOI":"10.1021\/ac201251s","article-title":"Real time analysis of brain tissue by direct combination of ultrasonic surgical aspiration and sonic spray mass spectrometry","volume":"83","author":"Sch\u00e4fer K. C.","year":"2011","unstructured":"K. C. Sch\u00e4fer ., Real time analysis of brain tissue by direct combination of ultrasonic surgical aspiration and sonic spray mass spectrometry. Anal. Chem. 83, 7729\u20137735 (2011).","journal-title":"Anal. Chem."},{"key":"e_1_3_4_28_2","unstructured":"M. Tzafetas Data from \u201cREIMS cervical tissue dataset.\u201d Figshare. https:\/\/figshare.com\/articles\/N_vs_HPV_CIN_vs_Ca-ALL_Samples_data_csv\/11367458\/1. Accessed 13 December 2019."},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1002\/jso.24108","article-title":"Margins for cervical and vulvar cancer","volume":"113","author":"Khanna N.","year":"2016","unstructured":"N. Khanna, L. A. Rauh, M. P. Lachiewicz, I. R. Horowitz, Margins for cervical and vulvar cancer. J. Surg. Oncol. 113, 304\u2013309 (2016).","journal-title":"J. Surg. Oncol."},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1200\/JCO.2000.18.8.1606","article-title":"Concurrent chemotherapy and pelvic radiation therapy compared with pelvic radiation therapy alone as adjuvant therapy after radical surgery in high-risk early-stage cancer of the cervix","volume":"18","author":"Peters W. A.","year":"2000","unstructured":"W. A. Peters, 3rd ., Concurrent chemotherapy and pelvic radiation therapy compared with pelvic radiation therapy alone as adjuvant therapy after radical surgery in high-risk early-stage cancer of the cervix. J. Clin. Oncol. 18, 1606\u20131613 (2000).","journal-title":"J. Clin. Oncol."},{"key":"e_1_3_4_31_2","first-page":"CD007583","article-title":"Radiotherapy and chemoradiation after surgery for early cervical cancer","volume":"5","author":"Rogers L.","year":"2012","unstructured":"L. Rogers, S. S. Siu, D. Luesley, A. Bryant, H. O. Dickinson, Radiotherapy and chemoradiation after surgery for early cervical cancer. Cochrane Database Syst. Rev. 5, CD007583 (2012).","journal-title":"Cochrane Database Syst. Rev."},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/0360-3016(94)E0139-B","article-title":"The effects of radiotherapy and surgery on the sexual function of women treated for cervical cancer","volume":"31","author":"Flay L. D.","year":"1995","unstructured":"L. D. Flay, J. H. Matthews, The effects of radiotherapy and surgery on the sexual function of women treated for cervical cancer. Int. J. Radiat. Oncol. Biol. Phys. 31, 399\u2013404 (1995).","journal-title":"Int. J. Radiat. Oncol. Biol. Phys."},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1080\/0284186X.2017.1400684","article-title":"Radiotherapy for cervical cancer\u2013Impact on the vaginal epithelium and sexual function","volume":"57","author":"Hofsj\u00f6 A.","year":"2018","unstructured":"A. Hofsj\u00f6, K. Bergmark, B. Blomgren, H. Jahren, N. Bohm-Starke, Radiotherapy for cervical cancer\u2013Impact on the vaginal epithelium and sexual function. Acta Oncol. 57, 338\u2013345 (2018).","journal-title":"Acta Oncol."},{"key":"e_1_3_4_34_2","doi-asserted-by":"crossref","first-page":"f7361","DOI":"10.1136\/bmj.f7361","article-title":"Effect of ageing on cervical or vaginal cancer in Swedish women previously treated for cervical intraepithelial neoplasia grade 3: Population based cohort study of long term incidence and mortality","volume":"348","author":"Strander B.","year":"2014","unstructured":"B. Strander, J. H\u00e4llgren, P. Spar\u00e9n, Effect of ageing on cervical or vaginal cancer in Swedish women previously treated for cervical intraepithelial neoplasia grade 3: Population based cohort study of long term incidence and mortality. BMJ 348, f7361 (2014).","journal-title":"BMJ"},{"key":"e_1_3_4_35_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/1471-0528.13530","article-title":"Evidence supporting see-and-treat management of cervical intraepithelial neoplasia: A systematic review and meta-analysis","volume":"123","author":"Ebisch R. M.","year":"2016","unstructured":"R. M. Ebisch ., Evidence supporting see-and-treat management of cervical intraepithelial neoplasia: A systematic review and meta-analysis. BJOG 123, 59\u201366 (2016).","journal-title":"BJOG"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.ejogrb.2007.09.001","article-title":"Psychological distress associated with colposcopy: Patients\u2019 perception","volume":"139","author":"Tahseen S.","year":"2008","unstructured":"S. Tahseen, P. C. Reid, Psychological distress associated with colposcopy: Patients\u2019 perception. Eur. J. Obstet. Gynecol. Reprod. Biol. 139, 90\u201394 (2008).","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1016\/S1470-2045(17)30700-3","article-title":"Incomplete excision of cervical precancer as a predictor of treatment failure: A systematic review and meta-analysis","volume":"18","author":"Arbyn M.","year":"2017","unstructured":"M. Arbyn ., Incomplete excision of cervical precancer as a predictor of treatment failure: A systematic review and meta-analysis. Lancet Oncol. 18, 1665\u20131679 (2017).","journal-title":"Lancet Oncol."},{"key":"e_1_3_4_38_2","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.ygyno.2013.01.023","article-title":"Accuracy of intraoperative pathological examination of SLN in cervical cancer","volume":"130","author":"Mart\u00ednez A.","year":"2013","unstructured":"A. Mart\u00ednez ., Accuracy of intraoperative pathological examination of SLN in cervical cancer. Gynecol. Oncol. 130, 525\u2013529 (2013).","journal-title":"Gynecol. Oncol."},{"key":"e_1_3_4_39_2","first-page":"2962450","article-title":"Results from survey to assess current trends in surgical practice in the management of women with early stage cervical cancer within the BGCS community with an emphasis on routine frozen section examination","volume":"2017","author":"Gubbala K.","year":"2017","unstructured":"K. Gubbala ., Results from survey to assess current trends in surgical practice in the management of women with early stage cervical cancer within the BGCS community with an emphasis on routine frozen section examination. Int. J. Surg. Oncol. 2017, 2962450 (2017).","journal-title":"Int. J. Surg. Oncol."},{"key":"e_1_3_4_40_2","unstructured":"Canadian Cancer Trials Group A randomized phase III trial comparing radical hysterectomy and pelvic node dissection vs simple hysterectomy and pelvic node dissection in patients with low-risk early stage cervical cancer (SHAPE). https:\/\/clinicaltrials.gov\/ct2\/show\/NCT01658930. Accessed 15 June 2019."},{"key":"e_1_3_4_41_2","unstructured":"Public Health England \u201cNHS cervical screening programme: Colposcopy and program management\u201d (NHSCSP Publication No. 20 Public Health England London 2016)."},{"key":"e_1_3_4_42_2","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1159\/000333438","article-title":"Thermal artifact after three techniques of loop excision of the transformation zone: A comparative study","volume":"73","author":"Boonlikit S.","year":"2012","unstructured":"S. Boonlikit, M. Yanaranop, Thermal artifact after three techniques of loop excision of the transformation zone: A comparative study. Gynecol. Obstet. Invest. 73, 230\u2013235 (2012).","journal-title":"Gynecol. Obstet. Invest."},{"key":"e_1_3_4_43_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1097\/00004347-199904000-00003","article-title":"Artifact in cervical LLETZ specimens: Correlation with follow-up","volume":"18","author":"Ioffe O. B.","year":"1999","unstructured":"O. B. Ioffe, S. E. Brooks, R. B. De Rezende, S. G. Silverberg, Artifact in cervical LLETZ specimens: Correlation with follow-up. Int. J. Gynecol. Pathol. 18, 115\u2013121 (1999).","journal-title":"Int. J. Gynecol. Pathol."},{"key":"e_1_3_4_44_2","first-page":"976","article-title":"Large-loop excision of the transformation zone: Effect on the pathologic interpretation of resection margins","volume":"81","author":"Montz F. J.","year":"1993","unstructured":"F. J. Montz, C. H. Holschneider, L. D. Thompson, Large-loop excision of the transformation zone: Effect on the pathologic interpretation of resection margins. Obstet. Gynecol. 81, 976\u2013982 (1993).","journal-title":"Obstet. Gynecol."},{"key":"e_1_3_4_45_2","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1373\/clinchem.2018.299289","article-title":"Performance of the MasSpec Pen for rapid diagnosis of ovarian cancer","volume":"65","author":"Sans M.","year":"2019","unstructured":"M. Sans ., Performance of the MasSpec Pen for rapid diagnosis of ovarian cancer. Clin. Chem. 65, 674\u2013683 (2019).","journal-title":"Clin. Chem."},{"key":"e_1_3_4_46_2","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1242\/dmm.011338","article-title":"Hooked on fat: The role of lipid synthesis in cancer metabolism and tumour development","volume":"6","author":"Baenke F.","year":"2013","unstructured":"F. Baenke, B. Peck, H. Miess, A. Schulze, Hooked on fat: The role of lipid synthesis in cancer metabolism and tumour development. Dis. Model. Mech. 6, 1353\u20131363 (2013).","journal-title":"Dis. Model. Mech."},{"key":"e_1_3_4_47_2","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.clnu.2008.03.006","article-title":"Altered plasma phospholipid fatty acids and nutritional status in patients with uterine cervical cancer","volume":"27","author":"Lisboa A. Q.","year":"2008","unstructured":"A. Q. Lisboa, M. Rezende, M. I. Muniz-Junqueira, M. K. Ito, Altered plasma phospholipid fatty acids and nutritional status in patients with uterine cervical cancer. Clin. Nutr. 27, 371\u2013377 (2008).","journal-title":"Clin. Nutr."},{"key":"e_1_3_4_48_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0079-6832(73)90003-7","article-title":"Tumor lipids","volume":"13","author":"Bergelson L. D.","year":"1972","unstructured":"L. D. Bergelson, Tumor lipids. Prog. Chem. Fats Other Lipids 13, 1\u201359 (1972).","journal-title":"Prog. Chem. Fats Other Lipids"},{"key":"e_1_3_4_49_2","doi-asserted-by":"crossref","first-page":"4970","DOI":"10.1039\/C8AY01691G","article-title":"Glycerophospholipids and sphingolipids correlate with poor prognostic genotypes of human papillomavirus in cervical cancer: Global lipidomics analysis","volume":"10","author":"Hung C.-Y.","year":"2018","unstructured":"C.-Y. Hung, Glycerophospholipids and sphingolipids correlate with poor prognostic genotypes of human papillomavirus in cervical cancer: Global lipidomics analysis. Anal. Methods 10, 4970\u20134977 (2018).","journal-title":"Anal. Methods"},{"key":"e_1_3_4_50_2","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1038\/381800a0","article-title":"Suppression of ceramide-mediated programmed cell death by sphingosine-1-phosphate","volume":"381","author":"Cuvillier O.","year":"1996","unstructured":"O. Cuvillier ., Suppression of ceramide-mediated programmed cell death by sphingosine-1-phosphate. Nature 381, 800\u2013803 (1996).","journal-title":"Nature"},{"key":"e_1_3_4_51_2","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1186\/s12885-018-4392-8","article-title":"HPV-16 E7 expression up-regulates phospholipase D activity and promotes rapamycin resistance in a pRB-dependent manner","volume":"18","author":"Rabachini T.","year":"2018","unstructured":"T. Rabachini ., HPV-16 E7 expression up-regulates phospholipase D activity and promotes rapamycin resistance in a pRB-dependent manner. BMC Cancer 18, 485 (2018).","journal-title":"BMC Cancer"},{"key":"e_1_3_4_52_2","doi-asserted-by":"crossref","first-page":"5485","DOI":"10.1007\/s13277-015-4164-x","article-title":"Identification of phosphatidylcholine and lysophosphatidylcholine as novel biomarkers for cervical cancers in a prospective cohort study","volume":"37","author":"Yin M. Z.","year":"2016","unstructured":"M. Z. Yin ., Identification of phosphatidylcholine and lysophosphatidylcholine as novel biomarkers for cervical cancers in a prospective cohort study. Tumour Biol. 37, 5485\u20135492 (2016).","journal-title":"Tumour Biol."},{"key":"e_1_3_4_53_2","doi-asserted-by":"crossref","first-page":"4163","DOI":"10.1002\/jcb.26118","article-title":"The potential value of the PI3K\/Akt\/mTOR signaling pathway for assessing prognosis in cervical cancer and as a target for therapy","volume":"118","author":"Bahrami A.","year":"2017","unstructured":"A. Bahrami ., The potential value of the PI3K\/Akt\/mTOR signaling pathway for assessing prognosis in cervical cancer and as a target for therapy. J. Cell. Biochem. 118, 4163\u20134169 (2017).","journal-title":"J. Cell. Biochem."},{"key":"e_1_3_4_54_2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.ijgo.2009.02.012","article-title":"Revised FIGO staging for carcinoma of the vulva, cervix, and endometrium","volume":"105","author":"Pecorelli S.","year":"2009","unstructured":"S. Pecorelli, Revised FIGO staging for carcinoma of the vulva, cervix, and endometrium. Int. J. Gynaecol. Obstet. 105, 103\u2013104 (2009).","journal-title":"Int. J. Gynaecol. Obstet."},{"key":"e_1_3_4_55_2","doi-asserted-by":"crossref","first-page":"8240","DOI":"10.1002\/anie.200902546","article-title":"In vivo, in situ tissue analysis using rapid evaporative ionization mass spectrometry","volume":"48","author":"Sch\u00e4fer K. C.","year":"2009","unstructured":"K. C. Sch\u00e4fer ., In vivo, in situ tissue analysis using rapid evaporative ionization mass spectrometry. Angew. Chem. Int. Ed. Engl. 48, 8240\u20138242 (2009).","journal-title":"Angew. Chem. Int. Ed. Engl."}],"updated-by":[{"DOI":"10.1073\/pnas.2013655117","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T00:00:00Z","timestamp":1596412800000}}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1916960117","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1916960117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T17:07:42Z","timestamp":1654621662000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1916960117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,16]]},"references-count":55,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2020,3,31]]}},"alternative-id":["10.1073\/pnas.1916960117"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1916960117","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,16]]},"assertion":[{"value":"2020-03-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}