{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:17:20Z","timestamp":1793146640153,"version":"4.5.0"},"reference-count":30,"publisher":"National Academy of Sciences","issue":"19","license":[{"start":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T00:00:00Z","timestamp":1603756800000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["Z-DK075032"],"award-info":[{"award-number":["Z-DK075032"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,5,12]]},"abstract":"<jats:p>\n                    HIV-1 maturation involves conversion of the immature Gag polyprotein lattice, which lines the inner surface of the viral membrane, to the mature capsid protein (CA) lattice, which encloses the viral RNA. Maturation inhibitors such as bevirimat (BVM) bind within six-helix bundles, formed by a segment that spans the junction between the CA and spacer peptide 1 (SP1) subunits of Gag, and interfere with cleavage between CA and SP1 catalyzed by the HIV-1 protease (PR). We report solid-state NMR (ssNMR) measurements on spherical virus-like particles (VLPs), facilitated by segmental isotopic labeling, that provide information about effects of BVM on the structure and dynamics of CA\u2013SP1 junction helices in the immature lattice. Although BVM strongly blocks PR-catalyzed CA\u2013SP1 cleavage in VLPs and blocks conversion of VLPs to tubular CA assemblies,\n                    <jats:sup>15<\/jats:sup>\n                    N and\n                    <jats:sup>13<\/jats:sup>\n                    C ssNMR chemical shifts of segmentally labeled VLPs with and without BVM are very similar, indicating that interaction with BVM does not alter the six-helix bundle structure appreciably. Only the\n                    <jats:sup>15<\/jats:sup>\n                    N chemical shift of A280 (the first residue of SP1) changes significantly, consistent with BVM binding to an internal ring of hydrophobic side chains of L279 residues. Measurements of transverse\n                    <jats:sup>15<\/jats:sup>\n                    N spin relaxation rates reveal a reduction in the amplitudes and\/or timescales of backbone N\u2013H bond motions, corresponding to a rigidification of the six-helix bundles. Overall, our data show that inhibition of HIV-1 maturation by BVM involves changes in structure and dynamics that are surprisingly subtle, but still sufficient to produce a large effect on CA\u2013SP1 cleavage.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1917755117","type":"journal-article","created":{"date-parts":[[2020,4,27]],"date-time":"2020-04-27T20:25:07Z","timestamp":1588019107000},"page":"10286-10293","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":25,"title":["Effects of an HIV-1 maturation inhibitor on the structure and dynamics of CA-SP1 junction helices in virus-like particles"],"prefix":"10.1073","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0346-6888","authenticated-orcid":false,"given":"Sebanti","family":"Gupta","sequence":"first","affiliation":[{"name":"Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Louis","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Tycko","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2020,4,27]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/S0960-9822(06)00331-9","article-title":"Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle","volume":"7","author":"Fuller S. 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