{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T10:51:23Z","timestamp":1789901483592,"version":"4.0.1"},"reference-count":57,"publisher":"National Academy of Sciences","issue":"27","license":[{"start":{"date-parts":[[2020,6,22]],"date-time":"2020-06-22T00:00:00Z","timestamp":1592784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Max Planck Gesellschaft","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,7,7]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Random errors and genetic lesions give rise to messenger RNAs encoding nonfunctional or toxic protein variants. An evolutionarily conserved cellular surveillance mechanism recognizes such aberrant transcripts and subjects them to destruction, a process known as nonsense-mediated decay (NMD). The UPF1 protein is an essential component of this NMD machinery. In a genetic screen aimed at identifying regulators of T cell development in zebrafish, we discovered a\n                    <jats:italic>upf1<\/jats:italic>\n                    mutation that causes widespread changes in the patterns of pre-mRNA splicing. Among the mRNA splicing regulators whose transcripts were affected by the mutation, the noncanonical splicing factor rpl10a was identified as a determinant of T cell development, exemplifying the advantages of examining NMD deficiency in the context of the whole vertebrate organism.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1917812117","type":"journal-article","created":{"date-parts":[[2020,6,22]],"date-time":"2020-06-22T20:36:20Z","timestamp":1592858180000},"page":"15799-15808","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":16,"title":["Pervasive changes of mRNA splicing in\n                    <i>upf1<\/i>\n                    -deficient zebrafish identify\n                    <i>rpl10a<\/i>\n                    as a regulator of T cell development"],"prefix":"10.1073","volume":"117","author":[{"given":"Divine-Fondzenyuy","family":"Lawir","sequence":"first","affiliation":[{"name":"Department of Developmental Immunology, Max-Planck Institute of Immunobiology and Epigenetics, D-79108 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4523-3524","authenticated-orcid":false,"given":"Katarzyna","family":"Sikora","sequence":"additional","affiliation":[{"name":"Department of Developmental Immunology, Max-Planck Institute of Immunobiology and Epigenetics, D-79108 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2007-8126","authenticated-orcid":false,"given":"Connor P.","family":"O\u2019Meara","sequence":"additional","affiliation":[{"name":"Department of Developmental Immunology, Max-Planck Institute of Immunobiology and Epigenetics, D-79108 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2067-2480","authenticated-orcid":false,"given":"Michael","family":"Schorpp","sequence":"additional","affiliation":[{"name":"Department of Developmental Immunology, Max-Planck Institute of Immunobiology and Epigenetics, D-79108 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Boehm","sequence":"additional","affiliation":[{"name":"Department of Developmental Immunology, Max-Planck Institute of Immunobiology and Epigenetics, D-79108 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2020,6,22]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.10.3924"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0126-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm4063"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.057919.116"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1064-z"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020203"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-9-r101"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.4161\/rna.22360"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2160905"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808902106"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1429"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0371287"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1382806"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.04.018"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg1015"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki905"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00361-08"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.7.1785"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05676"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.12a.2303"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3454"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2003.00070.x"},{"key":"e_1_3_3_23_2","first-page":"119","article-title":"Genetic dissection of thymus development","volume":"251","author":"Schorpp M.","year":"2000","unstructured":"M. Schorpp ., Genetic dissection of thymus development. Curr. Top. Microbiol. Immunol. 251, 119\u2013124 (2000).","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.11.003"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.070136.118"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.606911"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1767209"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv668"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2014.05.015"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep44145"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00177-09"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1319"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.06.031"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.030247.111"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.12.034"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw152"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1862"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.063420.117"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01689-08"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx508"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2012-13-5-r35"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msv002"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1320755111"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genom-082509-141730"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019352108"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.468808"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.01.018"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.04.012"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1600815"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071762"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406252102"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.4.2463"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.207"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1419161111"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1754"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz114"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1917812117","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1917812117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T05:04:15Z","timestamp":1649826255000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1917812117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,22]]},"references-count":57,"journal-issue":{"issue":"27","published-print":{"date-parts":[[2020,7,7]]}},"alternative-id":["10.1073\/pnas.1917812117"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1917812117","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,22]]},"assertion":[{"value":"2020-06-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}