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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,4,7]]},"abstract":"<jats:p>\n                    The spread of cholera in the midst of an epidemic is largely driven by direct transmission from person to person, although it is well-recognized that\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    is also capable of growth and long-term survival in aquatic ecosystems. While prior studies have shown that aquatic reservoirs are important in the persistence of the disease on the Indian subcontinent, an epidemiological view postulating that locally evolving environmental\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    contributes to outbreaks outside Asia remains debated. The single-source introduction of toxigenic\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O1 in Haiti, one of the largest outbreaks occurring this century, with 812,586 suspected cases and 9,606 deaths reported through July 2018, provided a unique opportunity to evaluate the role of aquatic reservoirs and assess bacterial transmission dynamics across environmental boundaries. To this end, we investigated the phylogeography of both clinical and aquatic toxigenic\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O1 isolates and show robust evidence of the establishment of aquatic reservoirs as well as ongoing evolution of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    isolates from aquatic sites. Novel environmental lineages emerged from sequential population bottlenecks, carrying mutations potentially involved in adaptation to the aquatic ecosystem. Based on such empirical data, we developed a mixed-transmission dynamic model of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    , where aquatic reservoirs actively contribute to genetic diversification and epidemic emergence, which underscores the complexity of transmission pathways in epidemics and endemic settings and the need for long-term investments in cholera control at both human and environmental levels.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1918763117","type":"journal-article","created":{"date-parts":[[2020,3,30]],"date-time":"2020-03-30T20:35:46Z","timestamp":1585600546000},"page":"7897-7904","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":54,"title":["Toxigenic\n                    <i>Vibrio cholerae<\/i>\n                    evolution and establishment of reservoirs in aquatic ecosystems"],"prefix":"10.1073","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3819-5520","authenticated-orcid":false,"given":"Carla","family":"Mavian","sequence":"first","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610;"},{"name":"Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6024-2744","authenticated-orcid":false,"given":"Taylor K.","family":"Paisie","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610;"},{"name":"Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meer T.","family":"Alam","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron","family":"Browne","sequence":"additional","affiliation":[{"name":"Mathematics Department, University of Louisiana, Lafayette, LA 70504;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valery Madsen","family":"Beau De Rochars","sequence":"additional","affiliation":[{"name":"Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610;"},{"name":"Department of Health Services Research, Management and Policy, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Nembrini","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610;"},{"name":"Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie N.","family":"Cash","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610;"},{"name":"Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610;"},{"name":"Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610;"},{"name":"Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL 32610;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taj","family":"Azarian","sequence":"additional","affiliation":[{"name":"Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 32827;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afsar","family":"Ali","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610;"},{"name":"Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"J. 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