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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,3,31]]},"abstract":"<jats:p>\n                    After being ingested by a female\n                    <jats:italic>Anopheles<\/jats:italic>\n                    mosquito during a bloodmeal on an infected host, and before they can reach the mosquito salivary glands to be transmitted to a new host,\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    parasites must establish an infection of the mosquito midgut in the form of oocysts. To achieve this, they must first survive a series of robust innate immune responses that take place prior to, during, and immediately after ookinete traversal of the midgut epithelium. Understanding how parasites may evade these responses could highlight new ways to block malaria transmission. We show that an ookinete and sporozoite surface protein designated as PIMMS43 (\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    Infection of the Mosquito Midgut Screen 43) is required for parasite evasion of the\n                    <jats:italic>Anopheles coluzzii<\/jats:italic>\n                    complement-like response. Disruption of PIMMS43 in the rodent malaria parasite\n                    <jats:italic>Plasmodium berghei<\/jats:italic>\n                    triggers robust complement activation and ookinete elimination upon mosquito midgut traversal. Silencing components of the complement-like system through RNAi largely restores ookinete-to-oocyst transition but oocysts remain small in size and produce a very small number of sporozoites that additionally are not infectious, indicating that PIMMS43 is also essential for sporogonic development in the oocyst. Antibodies that bind PIMMS43 interfere with parasite immune evasion when ingested with the infectious blood meal and significantly reduce the prevalence and intensity of infection. PIMMS43 genetic structure across African\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    populations indicates allelic adaptation to sympatric vector populations. These data add to our understanding of mosquito\u2013parasite interactions and identify PIMMS43 as a target of malaria transmission blocking.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1919709117","type":"journal-article","created":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T19:30:29Z","timestamp":1584041429000},"page":"7363-7373","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":54,"title":["PIMMS43 is required for malaria parasite immune evasion and sporogonic development in the mosquito vector"],"prefix":"10.1073","volume":"117","author":[{"given":"Chiamaka V.","family":"Ukegbu","sequence":"first","affiliation":[{"name":"Department of Life Sciences, Imperial College London, SW7 2AZ London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Giorgalli","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, SW7 2AZ London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Tapanelli","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, SW7 2AZ London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa D. 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