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MCMV is a surrogate to model host-pathogen interactions; however, the precise mechanism of MCMV entry remains incomplete. Here, we utilized a pronecroptotic form of MCMV as a strategy to perform a genome-wide forward genetic screen to identify host factors essential for infection. We characterize VEGF receptor Nrp-1 as a novel mediator of MCMV infection required for viral gene expression, replication, and necroptosis induction. Our observations define Nrp-1 as a key cellular factor that facilitates MCMV infection and may be an entry receptor for the virus.<\/jats:p>","DOI":"10.1073\/pnas.1921315117","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T20:25:46Z","timestamp":1596486346000},"page":"20109-20116","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":34,"title":["Necroptosis-based CRISPR knockout screen reveals Neuropilin-1 as a critical host factor for early stages of murine cytomegalovirus infection"],"prefix":"10.1073","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5233-5422","authenticated-orcid":false,"given":"Rebecca K.","family":"Lane","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Texas Health at San Antonio, San Antonio, TX 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