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We show that\n                    <jats:italic>S. aureus<\/jats:italic>\n                    requires aspartate biosynthesis to survive during bone infection, despite possessing an aspartate transporter, due to inhibition of aspartate utilization by the amino acid glutamate. Our results reveal a crucial role for inflammation-associated shifts in the host nutrient milieu for determining the metabolic pathways utilized by\n                    <jats:italic>S. aureus<\/jats:italic>\n                    during invasive infection.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1922211117","type":"journal-article","created":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T20:19:14Z","timestamp":1589573954000},"page":"12394-12401","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":80,"title":["Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive\n                    <i>Staphylococcus aureus<\/i>\n                    infection"],"prefix":"10.1073","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1473-1149","authenticated-orcid":false,"given":"Aimee D.","family":"Potter","sequence":"first","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8236-7281","authenticated-orcid":false,"given":"Casey E.","family":"Butrico","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3710-8037","authenticated-orcid":false,"given":"Caleb A.","family":"Ford","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9242-0461","authenticated-orcid":false,"given":"Jacob M.","family":"Curry","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina A.","family":"Trenary","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN 37235;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srivarun S.","family":"Tummarakota","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew S.","family":"Hendrix","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0871-1494","authenticated-orcid":false,"given":"Jamey D.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN 37235;"},{"name":"Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1199-1167","authenticated-orcid":false,"given":"James E.","family":"Cassat","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232;"},{"name":"Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235;"},{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232;"},{"name":"Vanderbilt Institute for Infection, Immunology, and Inflammation (VI4), Vanderbilt University Medical Center, Nashville, TN 37232;"},{"name":"Vanderbilt Center for Bone Biology, Vanderbilt University Medical Center, Nashville, TN 37232"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2020,5,15]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1086\/499632"},{"key":"e_1_3_3_2_2","first-page":"MBP","article-title":"Regulating the intersection of metabolism and pathogenesis in gram-positive bacteria","volume":"3","author":"Richardson A. 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