{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:45:52Z","timestamp":1791942352028,"version":"4.3.4"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"19","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,9,17]]},"abstract":"<jats:p>\n                    The\n                    <jats:italic>Brucella<\/jats:italic>\n                    BvrR\/BvrS two-component regulatory system is homologous to the ChvI\/ChvG systems of\n                    <jats:italic>Sinorhizobium<\/jats:italic>\n                    <jats:italic>meliloti<\/jats:italic>\n                    and\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    <jats:italic>tumefaciens<\/jats:italic>\n                    necessary for endosymbiosis and pathogenicity in plants. BvrR\/BvrS controls cell invasion and intracellular survival. Probing the surface of\n                    <jats:italic>bvrR<\/jats:italic>\n                    and\n                    <jats:italic>bvrS<\/jats:italic>\n                    transposon mutants with monoclonal antibodies showed all described major outer membrane proteins (Omps) but Omp25, a protein known to be involved in\n                    <jats:italic>Brucella<\/jats:italic>\n                    virulence. Absence of Omp25 expression was confirmed by two-dimensional electrophoresis of envelope fractions and by gene reporter studies. The electrophoretic analysis also revealed reduction or absence in the mutants of a second set of protein spots that by matrix-assisted laser desorption ionization MS and peptide mass mapping were identified as a non-previously described Omp (Omp3b). Because\n                    <jats:italic>bvrR<\/jats:italic>\n                    and\n                    <jats:italic>bvrS<\/jats:italic>\n                    mutants are also altered in cell-surface hydrophobicity, permeability, and sensitivity to surface-targeted bactericidal peptides, it is proposed that BvrR\/BvrS controls cell envelope changes necessary to transit between extracellular and intracellular environments. A genomic search revealed that Omp25 (Omp3a) and Omp3b belong to a family of Omps of plant and animal cell-associated \u03b1-\n                    <jats:italic>Proteobacteria<\/jats:italic>\n                    , which includes\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    <jats:italic>leguminosarum<\/jats:italic>\n                    RopB and\n                    <jats:italic>A.<\/jats:italic>\n                    <jats:italic>tumefaciens<\/jats:italic>\n                    AopB. Previous work has shown that RopB is not expressed in bacteroids, that AopB is involved in tumorigenesis, and that dysfunction of\n                    <jats:italic>A.<\/jats:italic>\n                    <jats:italic>tumefaciens<\/jats:italic>\n                    ChvI\/ChvG alters surface properties. It is thus proposed that the BvrR\/BvrS and Omp3 homologues of the cell-associated \u03b1-\n                    <jats:italic>Proteobacteria<\/jats:italic>\n                    play a role in bacterial surface control and host cell interactions.\n                  <\/jats:p>","DOI":"10.1073\/pnas.192439399","type":"journal-article","created":{"date-parts":[[2002,9,17]],"date-time":"2002-09-17T12:40:55Z","timestamp":1032266455000},"page":"12375-12380","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":165,"title":["The two-component system BvrR\/BvrS essential for\n                    <i>Brucella<\/i>\n                    <i>abortus<\/i>\n                    virulence regulates the expression of outer membrane proteins with counterparts in members of the\n                    <i>Rhizobiaceae<\/i>"],"prefix":"10.1073","volume":"99","author":[{"given":"C.","family":"Guzm\u00e1n-Verri","sequence":"first","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Manterola","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Sola-Landa","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Parra","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Cloeckaert","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Garin","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-P.","family":"Gorvel","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Moriy\u00f3n","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Moreno","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"L\u00f3pez-Go\u00f1i","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; Microbiology and Tumorbiology Center, Karolinska Institute, Box 280, S-171 77 Stockholm, Sweden; Departamento de Microbiolog\u00eda, Universidad de Navarra, 31008 Pamplona, Spain; Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France; CEA\/Grenoble, Laboratoire de Chimie des Proteines, 17 Rue des..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2002,9,6]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.022622699"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00913.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.20.5867-5876.1996"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.8.3054-3061.1995"},{"key":"e_1_3_3_5_2","first-page":"19","volume":"1","author":"Moriy\u00f3n I.","year":"1998","unstructured":"Moriy\u00f3n I. & L\u00f3pez-Go\u00f1i, I. 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