{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:53:02Z","timestamp":1790157182297,"version":"4.0.1"},"reference-count":38,"publisher":"National Academy of Sciences","issue":"19","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,9,16]]},"abstract":"<jats:p>\n                    Yeast strains with mutations in both\n                    <jats:italic>TEL1<\/jats:italic>\n                    and\n                    <jats:italic>MEC1<\/jats:italic>\n                    have short telomeres and elevated rates of chromosome deletions. By using a PCR assay, we demonstrate that\n                    <jats:italic>mec1 tel1<\/jats:italic>\n                    strains also have telomere\u2013telomere fusions (T-TFs). T-TFs require Lig4p (a ligase required for nonhomologous end-joining DNA repair). The highest rates of T-TFs are found in strains with combination of mutations that affect telomere length and DNA damage checkpoints (\n                    <jats:italic>mec1 tel1<\/jats:italic>\n                    ,\n                    <jats:italic>mec3 tel1<\/jats:italic>\n                    ,\n                    <jats:italic>mre11 mec1<\/jats:italic>\n                    , and\n                    <jats:italic>ddc1 tel1<\/jats:italic>\n                    strains). Examining many mutant genotypes, we find good agreement between the level of T-TFs and the rate of chromosomal deletions. In addition, if telomeres are elongated in a\n                    <jats:italic>mec1 tel1<\/jats:italic>\n                    strain, we eliminate T-TFs and reduce the deletion rate. The correlation between the level of T-TFs and the rate of deletions argues that many of these deletions reflect a cycle of T-TF formation (resulting in dicentric chromosomes), followed by chromosome breakage.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1934561100","type":"journal-article","created":{"date-parts":[[2003,9,16]],"date-time":"2003-09-16T13:42:33Z","timestamp":1063719753000},"page":"10854-10859","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":90,"title":["Genetic regulation of telomere-telomere fusions in the yeast\n                    <i>Saccharomyces cerevisae<\/i>"],"prefix":"10.1073","volume":"100","author":[{"given":"Piotr A.","family":"Mieczkowski","sequence":"first","affiliation":[{"name":"Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599-3280"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna O.","family":"Mieczkowska","sequence":"additional","affiliation":[{"name":"Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599-3280"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Dominska","sequence":"additional","affiliation":[{"name":"Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599-3280"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas D.","family":"Petes","sequence":"additional","affiliation":[{"name":"Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599-3280"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2003,9,8]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205080"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205060"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205061"},{"key":"e_1_3_2_4_2","first-page":"6054","volume":"19","year":"1999","unstructured":"Ritchie, K. 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