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U.S.A."],"published-print":{"date-parts":[[2020,7,28]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    The presence of a dominant male-determining locus (M-locus) in one of a pair of autosomes establishes the male sex in the dengue fever mosquito\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    . The\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    M-locus contains 30 genes, including\n                    <jats:italic>Nix<\/jats:italic>\n                    , a previously reported male-determining factor. Here we show that the\n                    <jats:italic>Nix<\/jats:italic>\n                    transgene alone was sufficient to convert females into fertile males, which continued to produce sex-converted progeny. We also show that a second M-locus gene named\n                    <jats:italic>myo-sex<\/jats:italic>\n                    was needed for male flight.\n                    <jats:italic>Nix<\/jats:italic>\n                    -mediated sex conversion was 100% penetrant, heritable, and stable, indicating great potential for developing mosquito-control strategies to reduce vector populations by female-to-male conversion. This work also sheds lights into the molecular basis of the function of the M-locus.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2001132117","type":"journal-article","created":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T20:30:05Z","timestamp":1594672205000},"page":"17702-17709","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":81,"title":["<i>Nix<\/i>\n                    alone is sufficient to convert female\n                    <i>Aedes aegypti<\/i>\n                    into fertile males and\n                    <i>myo-sex<\/i>\n                    is needed for male flight"],"prefix":"10.1073","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6469-8573","authenticated-orcid":false,"given":"Azadeh","family":"Aryan","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061;"},{"name":"Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1510-2942","authenticated-orcid":false,"given":"Michelle A. E.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061;"},{"name":"Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James K.","family":"Biedler","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061;"},{"name":"Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumin","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061;"},{"name":"Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin M.","family":"Overcash","sequence":"additional","affiliation":[{"name":"Department of Entomology, Texas A&amp;M University, College Station, TX 77843;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia N.","family":"Naumenko","sequence":"additional","affiliation":[{"name":"Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061;"},{"name":"Department of Entomology, Virginia Tech, Blacksburg, VA 24061;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5790-3548","authenticated-orcid":false,"given":"Maria V.","family":"Sharakhova","sequence":"additional","affiliation":[{"name":"Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061;"},{"name":"Department of Entomology, Virginia Tech, Blacksburg, VA 24061;"},{"name":"Laboratory of Ecology, Genetics, and Environment Protection, Tomsk State University, 634041 Tomsk, Russia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhong","family":"Mao","sequence":"additional","affiliation":[{"name":"Biocomplexity Institute &amp; Initiative, University of Virginia, Charlottesville, VA 22911"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5901-7171","authenticated-orcid":false,"given":"Zach N.","family":"Adelman","sequence":"additional","affiliation":[{"name":"Department of Entomology, Texas A&amp;M University, College Station, TX 77843;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4227-3819","authenticated-orcid":false,"given":"Zhijian","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061;"},{"name":"Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2020,7,13]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001899"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.4161\/fly.4.1.11277"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07052"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13315"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a109271"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/144.2.737"},{"key":"e_1_3_3_7_2","first-page":"1653","article-title":"Production of all female progeny: Evidence for the presence of the male sex determination factor on the Y chromosome","volume":"217","author":"Shukla J. 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