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U.S.A."],"published-print":{"date-parts":[[2020,10,13]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Digital PCR (dPCR) has long been recognized as a powerful tool in nucleic acid quantitative analysis, but there still remain problems that hinder its wide popularity. Sample loss is one major challenge that compromises the certainty of dPCR quantification and handicaps its ability to detect and quantify nucleic samples of low copy number. Another main difficulty is to prevent this single-copy-sensitivity experiment from possible contaminations of PCR product exposure. The CLEAR-dPCR introduced herein solves these two problems by integrating optically cleared monodisperse droplet generation and light-sheet fluorescence microscopy and fulfills a genuinely lossless and contamination-free digital PCR approach. 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