{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:06:30Z","timestamp":1790759190475,"version":"4.1.0"},"reference-count":65,"publisher":"National Academy of Sciences","issue":"29","license":[{"start":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T00:00:00Z","timestamp":1609891200000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"name":"HKU Seed fund for basic research","award":["201711159185"],"award-info":[{"award-number":["201711159185"]}]},{"name":"HKU Seed Fund for Basic Research","award":["201611159269"],"award-info":[{"award-number":["201611159269"]}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["Starting funds"],"award-info":[{"award-number":["Starting funds"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Hong Kong Outsanding Young-Researcher Award","award":["2015-2016"],"award-info":[{"award-number":["2015-2016"]}]},{"name":"University of Hong Kong Outstanding Research Student Supervisor Award","award":["2016-2017"],"award-info":[{"award-number":["2016-2017"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,7,21]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    We report the identification of a cubane-modified aptamer (cubamer) against the malaria biomarker\n                    <jats:italic>Plasmodium vivax<\/jats:italic>\n                    lactate dehydrogenase (PvLDH). The cubamer contains the benzene isostere cubane, which is entirely alien to biology. The crystal structure of the cubamer\u2013protein complex reveals a binding mechanism involving the formation of an unprecedented cubane pocket and an unusual C\u2013H\u22c5\u22c5\u22c5O hydrogen bond. Importantly, the cubamer is capable of distinguishing the PvLDH from the\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    LDH despite a very high sequence homology, which is impossible for unmodified aptamers. Finally, we have used the cubamer to detect PvLDH in a mimetic clinical situation. This approach blending medicinal chemistry and Darwinian evolution can easily be extended to other nonnatural, exotic functional groups.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2003267117","type":"journal-article","created":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T20:25:36Z","timestamp":1594067136000},"page":"16790-16798","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":90,"title":["Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker"],"prefix":"10.1073","volume":"117","author":[{"given":"Yee-Wai","family":"Cheung","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7947-2489","authenticated-orcid":false,"given":"Pascal","family":"R\u00f6thlisberger","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Laboratory for Bioorganic Chemistry of Nucleic Acids, Department of Structural Biology and Chemistry, CNRS UMR-3523, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5305-7495","authenticated-orcid":false,"given":"Ariel E.","family":"Mechaly","sequence":"additional","affiliation":[{"name":"Crystallography Platform-C2RT, Department of Structural Biology and Chemistry, CNRS UMR-3528, Institut Pasteur, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Weber","sequence":"additional","affiliation":[{"name":"Crystallography Platform-C2RT, Department of Structural Biology and Chemistry, CNRS UMR-3528, Institut Pasteur, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Levi-Acobas","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Laboratory for Bioorganic Chemistry of Nucleic Acids, Department of Structural Biology and Chemistry, CNRS UMR-3523, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1915-9666","authenticated-orcid":false,"given":"Young","family":"Lo","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvin W. 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