{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T16:41:09Z","timestamp":1793032869399,"version":"4.5.0"},"reference-count":38,"publisher":"National Academy of Sciences","issue":"33","license":[{"start":{"date-parts":[[2021,2,3]],"date-time":"2021-02-03T00:00:00Z","timestamp":1612310400000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81861148030"],"award-info":[{"award-number":["81861148030"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,8,18]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    t(8;21) acute myelogenous leukemia (AML) often presents different degrees of aberrant myeloid differentiation. Although most patients respond to current chemotherapy regimens, a sizeable number of patients are prone to relapse. Thus, the cellular heterogeneity and disease outcome may need exploration. Here, by integrating high-throughput DNA\/RNA sequencing with phenotypic analysis, heterogeneous leukemic cell populations were identified. CD34\n                    <jats:sup>+<\/jats:sup>\n                    CD117\n                    <jats:sup>dim<\/jats:sup>\n                    myeloblasts had the characteristics of granulocyte-monocyte progenitors and might be responsible for drug-resistance. CD34\n                    <jats:sup>+<\/jats:sup>\n                    CD117\n                    <jats:sup>bri<\/jats:sup>\n                    and AM cells were blocked later than CD34\n                    <jats:sup>+<\/jats:sup>\n                    CD117\n                    <jats:sup>dim<\/jats:sup>\n                    cells, and AM cells were sensitive to chemotherapy. t(8;21) AML patients with a high proportion of CD34\n                    <jats:sup>+<\/jats:sup>\n                    CD117\n                    <jats:sup>dim<\/jats:sup>\n                    cells had inferior outcomes. The identification of the CD34\n                    <jats:sup>+<\/jats:sup>\n                    CD117\n                    <jats:sup>dim<\/jats:sup>\n                    proportion as a potential prognostic factor may lead to new tools for future tailored therapeutic strategies.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2003900117","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T20:28:22Z","timestamp":1596486502000},"page":"20117-20126","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":58,"title":["Multidimensional study of the heterogeneity of leukemia cells in t(8;21) acute myelogenous leukemia identifies the subtype with poor outcome"],"prefix":"10.1073","volume":"117","author":[{"given":"Lu","family":"Jiang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Ping","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ting","family":"Dai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"},{"name":"School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240 Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9504-3331","authenticated-orcid":false,"given":"Xiang-Qin","family":"Weng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Min","family":"Xiong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8053-0209","authenticated-orcid":false,"given":"Jin-Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"},{"name":"Key Laboratory of Ministry of Education, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 200240 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3789-1284","authenticated-orcid":false,"given":"Sai-Juan","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, National Research Center for Translational Medicine, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China;"},{"name":"Key Laboratory of Ministry of Education, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 200240 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2020,8,3]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-08-733196"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1516192"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3709"},{"key":"e_1_3_4_4_2","first-page":"193","article-title":"Acute myeloid leukemia M2b","volume":"84","author":"Yushu H.","year":"1999","unstructured":"H. Yushu, B. Shougeng, X. Zhijian, M. Yingchang, H. Z. Chao, Acute myeloid leukemia M2b. Haematologica 84, 193\u2013194 (1999).","journal-title":"Haematologica"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(96)00101-4"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.104"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1976.tb03563.x"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-01-549212"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-11-468348"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-07-295279"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.31.4310"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70281-5"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-3640"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.06.9500"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1254257"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad0501"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0096-5"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.01.031"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-05-852822"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402465"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1995.tb03297.x"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209181"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature20598"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1349-1"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-017-0003-y"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.05.011"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2859"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14049"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2558"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11790"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1717125115"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature22993"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-10-696054"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-10-696039"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-08-363291"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-01-202606"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0129-6"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2014.114884"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.2003900117","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2003900117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T06:09:41Z","timestamp":1649830181000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2003900117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,3]]},"references-count":38,"journal-issue":{"issue":"33","published-print":{"date-parts":[[2020,8,18]]}},"alternative-id":["10.1073\/pnas.2003900117"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2003900117","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,3]]},"assertion":[{"value":"2020-08-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}