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We provide evidence that genetically male pigs with a knockout of the SRY gene undergo sex reversal of the external and internal genitalia. This discovery of SRY as the main switch for sex determination in pigs may provide an alternative for surgical castration in pig production, preventing boar taint. As the pig shares many genetic, physiological, and anatomical similarities with humans, it also provides a suitable large animal model for human sex reversal syndromes, allowing for the development of new interventions for human sex disorders.<\/jats:p>","DOI":"10.1073\/pnas.2008743118","type":"journal-article","created":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T11:55:09Z","timestamp":1608724509000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":45,"title":["Knockout of the HMG domain of the porcine SRY gene causes sex reversal in gene-edited pigs"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9268-4053","authenticated-orcid":false,"given":"Stefanie","family":"Kurtz","sequence":"first","affiliation":[{"name":"Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Mariensee, 31535 Neustadt am R\u00fcbenberge, 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