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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,9,8]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    The Calvin cycle is the most important carbon fixation pathway in the biosphere. However, its carboxylating enzyme Rubisco also accepts oxygen, thus producing 2-phosphoglycolate. Phosphoglycolate salvage pathways were extensively studied in photoautotrophs but remain uncharacterized in chemolithoautotrophs using the Calvin cycle. Here, we study phosphoglycolate salvage in the chemolithoautotrophic model bacterium\n                    <jats:italic>Cupriavidus necator<\/jats:italic>\n                    H16. We demonstrate that this bacterium mainly reassimilates 2-phosphoglycolate via the glycerate pathway. Upon disruption of this pathway, a secondary route, which we term the malate cycle, supports photorespiration by completely oxidizing 2-phosphoglycolate to CO\n                    <jats:sub>2<\/jats:sub>\n                    . While the malate cycle was not previously known to metabolize 2-phosphoglycolate in nature, a bioinformatic analysis suggests that it may support phosphoglycolate salvage in diverse chemoautotrophic bacteria.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2012288117","type":"journal-article","created":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T19:45:21Z","timestamp":1597952721000},"page":"22452-22461","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":63,"title":["Phosphoglycolate salvage in a chemolithoautotroph using the Calvin cycle"],"prefix":"10.1073","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1593-0377","authenticated-orcid":false,"given":"Nico J.","family":"Claassens","sequence":"first","affiliation":[{"name":"Systems and Synthetic Metabolism Lab, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9094-5195","authenticated-orcid":false,"given":"Giovanni","family":"Scarinci","sequence":"additional","affiliation":[{"name":"Systems and Synthetic Metabolism Lab, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6130-6984","authenticated-orcid":false,"given":"Axel","family":"Fischer","sequence":"additional","affiliation":[{"name":"Systems and Synthetic Metabolism Lab, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9278-5479","authenticated-orcid":false,"given":"Avi I.","family":"Flamholz","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Newell","sequence":"additional","affiliation":[{"name":"Systems and Synthetic Metabolism Lab, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4141-7836","authenticated-orcid":false,"given":"Stefan","family":"Frielingsdorf","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Chemie, Physikalische Chemie, Technische Universita\u0308t Berlin, 10623 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4550-5128","authenticated-orcid":false,"given":"Oliver","family":"Lenz","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Chemie, Physikalische Chemie, Technische Universita\u0308t Berlin, 10623 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1039-4328","authenticated-orcid":false,"given":"Arren","family":"Bar-Even","sequence":"additional","affiliation":[{"name":"Systems and Synthetic Metabolism Lab, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2020,8,20]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.17.00256"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(71)90051-9"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2010.03.006"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1438-8677.2012.00693.x"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0807043105"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1139\/b05-067"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-010-9615-z"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.082982"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.41.3.422"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024913925002"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-67242-2_28"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.125.1.20"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.3354\/ame01315"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2018.01.019"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1424031112"},{"key":"e_1_3_4_16_2","doi-asserted-by":"crossref","unstructured":"J. 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