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The olivocochlear synapse involves \u03b19\u03b110 nicotinic acetylcholine receptors (nAChRs), which do not express in recombinant cell lines. Here, genome-wide screening determined that assembly and surface expression of \u03b19\u03b110 require ligand binding. Ion channel function additionally demands an auxiliary subunit, which can be transmembrane inner ear (TMIE) or TMEM132e. These single-pass transmembrane proteins are enriched in hair cells and underlie nonsyndromic human deafness. Inner hair cells from TMIE mutant mice show altered postsynaptic \u03b19\u03b110 function and abnormally retain \u03b19\u03b110-mediated transmission through development. This study identifies unexpected functions for human deafness genes TMIE\/TMEM132e and enables drug discovery for this elusive nAChR implicated in prevalent auditory disorders.<\/jats:p>","DOI":"10.1073\/pnas.2013762117","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T20:26:08Z","timestamp":1600115168000},"page":"24534-24544","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":37,"title":["Hair cell \u03b19\u03b110 nicotinic acetylcholine receptor functional expression regulated by ligand binding and deafness gene products"],"prefix":"10.1073","volume":"117","author":[{"given":"Shenyan","family":"Gu","sequence":"first","affiliation":[{"name":"Neuroscience Discovery, Janssen Pharmaceutical Companies of Johnson &amp; Johnson, San Diego, CA 92121"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Knowland","sequence":"additional","affiliation":[{"name":"Neuroscience Discovery, Janssen Pharmaceutical Companies of Johnson &amp; 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