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We here generated a mouse model of familial hyperaldosteronism type IV with a heterozygous gain-of-function mutation in a calcium channel gene (\n                    <jats:italic>Cacna1h<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>M1560V\/+<\/jats:italic>\n                    <\/jats:sup>\n                    ).\n                    <jats:italic>Cacna1h<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>M1560V\/+<\/jats:italic>\n                    <\/jats:sup>\n                    mice have about twofold elevated aldosterone:renin ratios (a screening parameter for primary aldosteronism) and elevated blood pressure, with an overall mild phenotype. Elevated adrenal aldosterone synthase expression in\n                    <jats:italic>Cacna1h<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>M1560V\/+<\/jats:italic>\n                    <\/jats:sup>\n                    mice is associated with increased intracellular calcium concentrations in glomerulosa cells. This model allows for the ex vivo analysis of calcium signaling in aldosterone-producing glomerulosa cells of the adrenal gland.\n                    <jats:italic>Cacna1h<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice have normal aldosterone synthase expression, with implications for the evaluation of CACNA1H as a therapeutic target.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2014876118","type":"journal-article","created":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T17:25:43Z","timestamp":1618939543000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":37,"title":["Enhanced Ca\n                    <sup>2+<\/sup>\n                    signaling, mild primary aldosteronism, and hypertension in a familial hyperaldosteronism mouse model (\n                    <i>Cacna1h<\/i>\n                    <sup>\n                      <i>M1560V\/+<\/i>\n                    <\/sup>\n                    )"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3334-3950","authenticated-orcid":false,"given":"Eric","family":"Seidel","sequence":"first","affiliation":[{"name":"Department of Nephrology, Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, 10115 Berlin, Germany;"},{"name":"Berlin Institute of Health at Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, 10117 Berlin, Germany;"},{"name":"Center for Regenerative Therapies, Berlin Institute of Health at Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, 13353 Berlin, Germany;"},{"name":"Department of Nephrology, School of Medicine, Heinrich-Heine Universit\u00e4t D\u00fcsseldorf, 40225 D\u00fcsseldorf, 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