{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T08:49:09Z","timestamp":1783414149223,"version":"3.54.6"},"reference-count":48,"publisher":"National Academy of Sciences","issue":"32","license":[{"start":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T00:00:00Z","timestamp":1644019200000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/501100004587","name":"MINECO | Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI16\/00038"],"award-info":[{"award-number":["PI16\/00038"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"MINECO | Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI19\/00188"],"award-info":[{"award-number":["PI19\/00188"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n S\u00e9neca","doi-asserted-by":"publisher","award":["19400\/PI\/14"],"award-info":[{"award-number":["19400\/PI\/14"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008054","name":"Fundaci\u00f3n Ram\u00f3n Areces","doi-asserted-by":"publisher","award":["00000"],"award-info":[{"award-number":["00000"]}],"id":[{"id":"10.13039\/100008054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["BIO2017-84702-R"],"award-info":[{"award-number":["BIO2017-84702-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["Juan de la Cierva postdoctoral contract"],"award-info":[{"award-number":["Juan de la Cierva postdoctoral contract"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004687","name":"Universidad de Murcia","doi-asserted-by":"publisher","award":["Postdoctoral contract"],"award-info":[{"award-number":["Postdoctoral contract"]}],"id":[{"id":"10.13039\/501100004687","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004687","name":"Universidad de Murcia","doi-asserted-by":"publisher","award":["postdoctoral contract"],"award-info":[{"award-number":["postdoctoral contract"]}],"id":[{"id":"10.13039\/501100004687","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004687","name":"Universidad de Murcia","doi-asserted-by":"publisher","award":["Predoctoral contract"],"award-info":[{"award-number":["Predoctoral contract"]}],"id":[{"id":"10.13039\/501100004687","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004687","name":"Universidad de Murcia","doi-asserted-by":"publisher","award":["Predoctoral contract"],"award-info":[{"award-number":["Predoctoral contract"]}],"id":[{"id":"10.13039\/501100004687","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,8,10]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Dyskeratosis congenita (DC) is a rare inherited bone marrow failure and cancer predisposition syndrome caused by mutations in telomerase or telomeric proteins. Although all patients show telomere shortening, the symptoms are highly variable and, therefore, other mechanisms have to be involved. Here, we report that telomerase RNA component (\n                    <jats:italic>TERC<\/jats:italic>\n                    ) regulates the expression of master myeloid genes in zebrafish and humans by recruiting the transcription machinery. Induced pluripotent stem cells derived from DC patients with a\n                    <jats:italic>TERC<\/jats:italic>\n                    mutation in a specific domain, the CR4-CR5, shows impaired myelopoiesis, while those from DC patients with mutation in telomerase catalytic subunit differentiated normally. Our findings reveal a target for personalized and precision medicine approaches for DC patients.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2015528118","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T16:25:15Z","timestamp":1628180715000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":15,"title":["Telomerase RNA recruits RNA polymerase II to target gene promoters to enhance myelopoiesis"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7520-6746","authenticated-orcid":false,"given":"Jes\u00fas","family":"Garc\u00eda-Castillo","sequence":"first","affiliation":[{"name":"Grupo de Telomerasa, C\u00e1ncer y Envejecimiento, Hospital Cl\u00ednico Universitario Virgen de la Arrixaca, 30120 Murcia, Spain;"},{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria-Arrixaca, 30120 Murcia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1802-4943","authenticated-orcid":false,"given":"Francisca","family":"Alcaraz-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Grupo de Telomerasa, C\u00e1ncer y Envejecimiento, Hospital Cl\u00ednico Universitario Virgen de la Arrixaca, 30120 Murcia, Spain;"},{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria-Arrixaca, 30120 Murcia, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras, Instituto de Salud Carlos III, 30100 Murcia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3287-2228","authenticated-orcid":false,"given":"Elena","family":"Mart\u00ednez-Balsalobre","sequence":"additional","affiliation":[{"name":"Grupo de Telomerasa, C\u00e1ncer y Envejecimiento, Hospital Cl\u00ednico Universitario Virgen de la Arrixaca, 30120 Murcia, Spain;"},{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria-Arrixaca, 30120 Murcia, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras, Instituto de Salud Carlos III, 30100 Murcia, Spain;"},{"name":"Departamento de Biolog\u00eda Celular e Histolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Garc\u00eda-Moreno","sequence":"additional","affiliation":[{"name":"Grupo de Telomerasa, C\u00e1ncer y Envejecimiento, Hospital Cl\u00ednico Universitario Virgen de la Arrixaca, 30120 Murcia, Spain;"},{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria-Arrixaca, 30120 Murcia, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras, Instituto de Salud Carlos III, 30100 Murcia, Spain;"},{"name":"Departamento de Biolog\u00eda Celular e Histolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2056-6792","authenticated-orcid":false,"given":"Marlies P.","family":"Rossmann","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138;"},{"name":"Stem Cell Program and Division of Hematology\/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Fern\u00e1ndez-Lajar\u00edn","sequence":"additional","affiliation":[{"name":"Grupo de Telomerasa, C\u00e1ncer y Envejecimiento, Hospital Cl\u00ednico Universitario Virgen de la Arrixaca, 30120 Murcia, Spain;"},{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria-Arrixaca, 30120 Murcia, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras, Instituto de Salud Carlos III, 30100 Murcia, Spain;"},{"name":"Departamento de Biolog\u00eda Celular e Histolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4334-0059","authenticated-orcid":false,"given":"Manuel","family":"Bernab\u00e9-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Grupo de Telomerasa, C\u00e1ncer y Envejecimiento, Hospital Cl\u00ednico Universitario Virgen de la Arrixaca, 30120 Murcia, Spain;"},{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria-Arrixaca, 30120 Murcia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2596-0871","authenticated-orcid":false,"given":"Ana B.","family":"P\u00e9rez-Oliva","sequence":"additional","affiliation":[{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria-Arrixaca, 30120 Murcia, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras, Instituto de Salud Carlos III, 30100 Murcia, Spain;"},{"name":"Departamento de Biolog\u00eda Celular e Histolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia C.","family":"Rodr\u00edguez-Cortez","sequence":"additional","affiliation":[{"name":"Josep Carreras Leukemia Research Institute, University of Barcelona, 08036 Barcelona, Spain;"},{"name":"Department of Biomedicine, School of Medicine, University of Barcelona, 08036 Barcelona, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer, Instituto de Salud Carlos III, 08036 Barcelona, Spain;"},{"name":"Instituci\u00f2 Catalana de Recerca i Estudis Avan\u00e7ats, 08036 Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1442-6216","authenticated-orcid":false,"given":"Clara","family":"Bueno","sequence":"additional","affiliation":[{"name":"Josep Carreras Leukemia Research Institute, University of Barcelona, 08036 Barcelona, Spain;"},{"name":"Department of Biomedicine, School of Medicine, University of Barcelona, 08036 Barcelona, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer, Instituto de Salud Carlos III, 08036 Barcelona, Spain;"},{"name":"Instituci\u00f2 Catalana de Recerca i Estudis Avan\u00e7ats, 08036 Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac","family":"Adatto","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138;"},{"name":"Stem Cell Program and Division of Hematology\/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suneet","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Stem Cell Program and Division of Hematology\/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115;"},{"name":"Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Men\u00e9ndez","sequence":"additional","affiliation":[{"name":"Josep Carreras Leukemia Research Institute, University of Barcelona, 08036 Barcelona, Spain;"},{"name":"Department of Biomedicine, School of Medicine, University of Barcelona, 08036 Barcelona, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer, Instituto de Salud Carlos III, 08036 Barcelona, Spain;"},{"name":"Instituci\u00f2 Catalana de Recerca i Estudis Avan\u00e7ats, 08036 Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0860-926X","authenticated-orcid":false,"given":"Leonard I.","family":"Zon","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138;"},{"name":"Stem Cell Program and Division of Hematology\/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115;"},{"name":"Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9527-0211","authenticated-orcid":false,"given":"Victoriano","family":"Mulero","sequence":"additional","affiliation":[{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria-Arrixaca, 30120 Murcia, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras, Instituto de Salud Carlos III, 30100 Murcia, Spain;"},{"name":"Departamento de Biolog\u00eda Celular e Histolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2021-9822","authenticated-orcid":false,"given":"Mar\u00eda L.","family":"Cayuela","sequence":"additional","affiliation":[{"name":"Grupo de Telomerasa, C\u00e1ncer y Envejecimiento, Hospital Cl\u00ednico Universitario Virgen de la Arrixaca, 30120 Murcia, Spain;"},{"name":"Instituto Murciano de Investigaci\u00f3n Biosanitaria-Arrixaca, 30120 Murcia, Spain;"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras, Instituto de Salud Carlos III, 30100 Murcia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2021,8,5]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.11.036"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri890"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021003"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2009.01.010"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1354009"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701009104"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024383"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404339"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0057"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2007.07.017"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2011.1.480"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0040010"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12943"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.182407599"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03836"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08137"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-019-0612-5"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky705"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4228"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.08.027"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-09-3659"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-12-475392"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-06-162495"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9146"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-05-024075"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.11.014"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.058248.116"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-04-710160"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5599"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2018.00345"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0028-8"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1192002"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/wsbm.148"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-051410-092902"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-014-0453-y"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-016-0615-1"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.459"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.07.017"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.02.019"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0199712"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21343"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1934"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-12-327403"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-8-81"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810738106"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.239"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016955"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks339"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.2015528118","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2015528118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T05:56:00Z","timestamp":1649829360000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2015528118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,5]]},"references-count":48,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2021,8,10]]}},"alternative-id":["10.1073\/pnas.2015528118"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2015528118","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,5]]},"assertion":[{"value":"2021-08-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2015528118"}}