{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:54:39Z","timestamp":1789610079683,"version":"build-2803163510"},"reference-count":73,"publisher":"National Academy of Sciences","issue":"48","license":[{"start":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T00:00:00Z","timestamp":1605484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    TRPML1 (transient receptor potential mucolipin 1) is a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -permeable, nonselective cation channel that is localized to late endosomes and lysosomes. Here, we investigated the function of TRPML1 channels in regulating lower urinary tract (LUT) smooth muscle cell (SMC) contractility. We found that TRPML1 forms a stable signaling complex with ryanodine receptors (RyRs) in the sarcoplasmic reticulum (SR). We further showed that TRPML1 channels are important for initiating an essential Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -signaling negative feedback mechanism between RyRs on SR membranes and K\n                    <jats:sup>+<\/jats:sup>\n                    channels on the plasma membrane. Knockout of TRPML1 channels in mice impaired this pathway, resulting in LUT smooth muscle hypercontractility and symptoms of overactive bladder. Our findings demonstrate a critical role for TRPML1 in LUT function.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2016959117","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T20:31:26Z","timestamp":1605558686000},"page":"30775-30786","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":29,"title":["The intracellular Ca\n                    <sup>2+<\/sup>\n                    release channel TRPML1 regulates lower urinary tract smooth muscle contractility"],"prefix":"10.1073","volume":"117","author":[{"given":"Caoimhin S.","family":"Griffin","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Center for Molecular and Cellular Signaling in the Cardiovascular System, Reno School of Medicine, University of Nevada, Reno, NV 89557-0318;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3489-9021","authenticated-orcid":false,"given":"Michael G.","family":"Alvarado","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Center for Molecular and Cellular Signaling in the Cardiovascular System, Reno School of Medicine, University of Nevada, Reno, NV 89557-0318;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Center for Molecular and Cellular Signaling in the Cardiovascular System, Reno School of Medicine, University of Nevada, Reno, NV 89557-0318;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard T.","family":"Drumm","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, Reno School of Medicine, University of Nevada, Reno, NV 89557-0318;"},{"name":"Department of Life &amp; 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