{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:43:32Z","timestamp":1790664212929,"version":"4.1.0"},"reference-count":48,"publisher":"National Academy of Sciences","issue":"32","license":[{"start":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T00:00:00Z","timestamp":1643846400000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1-T32-AI-141393-01"],"award-info":[{"award-number":["1-T32-AI-141393-01"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,8,10]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Nontypeable\n                    <jats:italic>Haemophilus influenzae<\/jats:italic>\n                    (NTHi) is a common cause of localized respiratory tract disease and results in significant morbidity in children and adults, prompting interest in developing a vaccine to prevent NTHi disease. It has been challenging to date to develop a vaccine that has broad activity against diverse NTHi strains. In this work, we found that immunization of mice with the NTHi HMW1 and HMW2 adhesive proteins stimulates protective immunity against nasopharyngeal colonization by genetically distinct NTHi strains. This immunity includes a strain-specific antibody response and a broadly protective cell-mediated response. Our results highlight the vaccine potential of the HMW1 and HMW2 proteins.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2019923118","type":"journal-article","created":{"date-parts":[[2021,8,3]],"date-time":"2021-08-03T15:35:12Z","timestamp":1628004912000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":16,"title":["Immunization with HMW1 and HMW2 adhesins protects against colonization by heterologous strains of nontypeable\n                    <i>Haemophilus influenzae<\/i>"],"prefix":"10.1073","volume":"118","author":[{"given":"Nadia A.","family":"Kadry","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1451-358X","authenticated-orcid":false,"given":"Eric A.","family":"Porsch","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5003-4992","authenticated-orcid":false,"given":"Hao","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104;"},{"name":"Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6787-9510","authenticated-orcid":false,"suffix":"III","given":"Joseph W.","family":"St. Geme","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104;"},{"name":"Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2021,8,3]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318184dba2"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012561"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.10.2175-2179.1989"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1999.tb00393.x"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6815-6-10"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199411000-00009"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01539-15"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.10.025"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01564-08"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.5.3482"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(00)90189-1"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000159"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900237"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200955"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.10.012"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00596-16"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.11.7980"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00084-13"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.1.364-368.1998"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.7.2875"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.13.4209-4217.2004"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03450.x"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.4.1302-1313.1992"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00153-16"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2014.09.035"},{"key":"e_1_3_4_26_2","first-page":"99","article-title":"Distribution and diversity of hmw1A among invasive nontypeable Haemophilus influenzae isolates in Iran","volume":"8","author":"Shahini Shams Abadi M.","year":"2016","unstructured":"M. Shahini Shams Abadi ., Distribution and diversity of hmw1A among invasive nontypeable Haemophilus influenzae isolates in Iran. Avicenna J. Med. Biotechnol. 8, 99\u2013102 (2016).","journal-title":"Avicenna J. Med. Biotechnol."},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199005000-00006"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00502-15"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0170884"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2014.55"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2016.71"},{"key":"e_1_3_4_32_2","first-page":"1899","article-title":"Cellular effectors mediating Th17-dependent clearance of pneumococcal colonization in mice","volume":"119","author":"Zhang Z.","year":"2009","unstructured":"Z. Zhang, T. B. Clarke, J. N. Weiser, Cellular effectors mediating Th17-dependent clearance of pneumococcal colonization in mice. J. Clin. Invest. 119, 1899\u20131909 (2009).","journal-title":"J. Clin. Invest."},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1802261115"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2011.00352.x"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042664"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8091342"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11518-0"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu106"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3365"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-019-0218-5"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.1.7-16.1990"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00396-07"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00851-07"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.3.761"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1086\/344233"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051718"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00215-18"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(95)00165-7"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.2019923118","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2019923118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T05:56:36Z","timestamp":1649829396000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2019923118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,3]]},"references-count":48,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2021,8,10]]}},"alternative-id":["10.1073\/pnas.2019923118"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2019923118","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,3]]},"assertion":[{"value":"2021-08-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2019923118"}}