{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:49:41Z","timestamp":1790408981696,"version":"4.1.0"},"reference-count":45,"publisher":"National Academy of Sciences","issue":"6","license":[{"start":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T00:00:00Z","timestamp":1628121600000},"content-version":"vor","delay-in-days":181,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,2,9]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Honey bees pollinate the majority of crops, but their survival is under threat. The bee parasitic mite\n                    <jats:italic>Varroa destructor<\/jats:italic>\n                    is a major cause of honey bee decline. The successful control of\n                    <jats:italic>Varroa<\/jats:italic>\n                    mites depends on a small number of in-hive acaricides. Species-specific activation of the chemical coumaphos is a powerful \u201cpro-drug\u201d approach to attain this selective toxicity and protect bees. We identified a coumaphos-resistant\n                    <jats:italic>Varroa<\/jats:italic>\n                    population that escapes toxicity by down-regulating the activating enzyme, a P450 monooxygenase. Decreased activation is a rare but evolutionarily powerful solution to achieve resistance. It demonstrates that the current proinsecticide approach is under threat, as it can be circumvented, and highlights the need to restart the pipeline to develop selective pest control agents.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2020380118","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T16:31:26Z","timestamp":1612801886000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":81,"title":["Reduced proinsecticide activation by cytochrome P450 confers coumaphos resistance in the major bee parasite\n                    <i>Varroa destructor<\/i>"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4074-6806","authenticated-orcid":false,"given":"Spyridon","family":"Vlogiannitis","sequence":"first","affiliation":[{"name":"Department of Crop Science, Agricultural University of Athens, GR-11855 Athens, 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Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5288-0308","authenticated-orcid":false,"given":"Simon","family":"Snoeck","sequence":"additional","affiliation":[{"name":"Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, B-9000 Ghent, Belgium;"},{"name":"Department of Biology, University of Washington, Seattle, WA 98195;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5176-4671","authenticated-orcid":false,"given":"Evangelia","family":"Katsavou","sequence":"additional","affiliation":[{"name":"Department of Crop Science, Agricultural University of Athens, GR-11855 Athens, Greece;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelia","family":"Morou","sequence":"additional","affiliation":[{"name":"Department of Crop Science, Agricultural University of Athens, GR-11855 Athens, Greece;"},{"name":"Institute of Molecular Biology and 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