{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T09:19:45Z","timestamp":1792919985165,"version":"4.5.0"},"reference-count":57,"publisher":"National Academy of Sciences","issue":"26","license":[{"start":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T00:00:00Z","timestamp":1624579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS096997"],"award-info":[{"award-number":["NS096997"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS106138"],"award-info":[{"award-number":["NS106138"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS097775"],"award-info":[{"award-number":["NS097775"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"HHS | NIH | National Institute on Aging","doi-asserted-by":"publisher","award":["AG063031"],"award-info":[{"award-number":["AG063031"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"HHS | NIH | National Institute on Aging","doi-asserted-by":"publisher","award":["AG062738"],"award-info":[{"award-number":["AG062738"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["20POST35160001"],"award-info":[{"award-number":["20POST35160001"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Luso-American Development Foundation","award":["2017\/165"],"award-info":[{"award-number":["2017\/165"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,6,29]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>The distribution of blood throughout the brain is facilitated by highly interconnected capillary networks. However, the steps involved in the construction of these networks has remained unclear. We used in vivo two-photon imaging through noninvasive cranial windows to study the engineering of capillary networks in the cerebral cortex of mouse neonates. We find that angiogenic activity originates at ascending venules, which undergo a burst of sprouting in the second postnatal week. This sprouting activity first establishes long paths to connect venules to blood input from neighboring arterioles, and then expands capillary interconnectivity with a multitude of short-range connections. Our study provides an experimental foundation to understand how capillary networks are shaped in the living mammalian brain during postnatal development.<\/jats:p>","DOI":"10.1073\/pnas.2100866118","type":"journal-article","created":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T15:56:07Z","timestamp":1624636567000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":85,"title":["Imaging the construction of capillary networks in the neonatal mouse brain"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9450-6103","authenticated-orcid":false,"given":"Vanessa","family":"Coelho-Santos","sequence":"first","affiliation":[{"name":"Center for Developmental Biology and Regenerative Medicine, Seattle Children\u2019s Research Institute, Seattle, WA 98101;"},{"name":"Department of Pediatrics, University of Washington, Seattle, WA 98195;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9e-Anne","family":"Berthiaume","sequence":"additional","affiliation":[{"name":"Center for Developmental Biology and Regenerative Medicine, Seattle Children\u2019s Research Institute, Seattle, WA 98101;"},{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC 29425;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9441-3030","authenticated-orcid":false,"given":"Sharon","family":"Ornelas","sequence":"additional","affiliation":[{"name":"Center for Developmental Biology and Regenerative Medicine, Seattle Children\u2019s Research Institute, Seattle, WA 98101;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Stuhlmann","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, Weill Cornell Medical College, New York, NY 10065;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy Y.","family":"Shih","sequence":"additional","affiliation":[{"name":"Center for Developmental Biology and Regenerative Medicine, Seattle Children\u2019s Research Institute, Seattle, WA 98101;"},{"name":"Department of Pediatrics, University of Washington, Seattle, WA 98195;"},{"name":"Department of Bioengineering, University of Washington, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2021,6,25]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10144"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16671146"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005392"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00793-2"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2011.153"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.2.4"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4533"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-019-0434-z"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4863-8_41"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200210000-00015"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1992.130"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2012.152"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.07.034"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08873"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.059881"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6758"},{"key":"e_1_3_4_17_2","first-page":"522","article-title":"Development of the mouse retinal vasculature: Angiogenesis versus vasculogenesis","volume":"43","author":"Fruttiger M.","year":"2002","unstructured":"M. Fruttiger, Development of the mouse retinal vasculature: Angiogenesis versus vasculogenesis. Invest. Ophthalmol. Vis. Sci. 43, 522\u2013527 (2002).","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201800198"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12238"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2013.04.001"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/376070a0"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/1526-968X(200010)28:2<75::AID-GENE50>3.0.CO;2-S"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.81.4.567"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-08982-z"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2011.95"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X15608388"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22781"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090455"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.14.3047"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09513"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09522"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.9.1591"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.NPh.2.4.041402"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17732229"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.363"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3426"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3278"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2011.196"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2008.166"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(92)90020-P"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13991"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12554"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13324"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1710848114"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.058776"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.03.026"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.12167"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12821"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1903-16.2016"},{"key":"e_1_3_4_50_2","volume-title":"Atlas of the Developing Mouse Brain at E17.5, P0 and P6","author":"Paxinos G.","year":"2007","unstructured":"G. Paxinos, G. M. Halliday, C. Watson, Y. Koutcherov, H. U. Wang, Atlas of the Developing Mouse Brain at E17.5, P0 and P6 (Elsevier, 2007)."},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X19831016"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.07.034"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-01-262386"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038590"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.21037\/qims-20-920"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027385"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.2100866118","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2100866118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T08:35:35Z","timestamp":1649838935000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2100866118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,25]]},"references-count":57,"journal-issue":{"issue":"26","published-print":{"date-parts":[[2021,6,29]]}},"alternative-id":["10.1073\/pnas.2100866118"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2100866118","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,25]]},"assertion":[{"value":"2021-06-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2100866118"}}