{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T21:33:50Z","timestamp":1787434430877,"version":"build-2736575974"},"reference-count":36,"publisher":"National Academy of Sciences","issue":"30","license":[{"start":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T00:00:00Z","timestamp":1642550400000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"name":"natioanl key research and development programe of China","award":["2018YFA0901900"],"award-info":[{"award-number":["2018YFA0901900"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871250"],"award-info":[{"award-number":["31871250"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31670034"],"award-info":[{"award-number":["31670034"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31870026"],"award-info":[{"award-number":["31870026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,7,27]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Transfer RNA (tRNA) transfer appropriately activated amino acids to the end of the growing peptide chain, playing significant roles in primary metabolism. Importantly, tRNA have also been shown to function outside of translation. In this report, we present the characterization of a new class of aminoacyl-tRNA (aa-tRNA)\u2013dependent enzyme, BlsK, from\n                    <jats:italic>Streptomyces griseochromogenes<\/jats:italic>\n                    . BlsK catalyzes an aa-tRNA\u2013dependent leucyl transfer reaction in blasticidin S biosynthesis. BlsK contains a [3Fe-4S] cluster that is necessary for its function and unprecedented in previously identified tRNA-dependent transferases. Because BlsK does not show similarity to any known functional protein, it may represent a type of aa-tRNA\u2013dependent enzyme. As such, this work has broad implications for both tRNA-dependent and iron\u2013sulfur\u2013containing enzymes.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2102318118","type":"journal-article","created":{"date-parts":[[2021,7,19]],"date-time":"2021-07-19T16:31:06Z","timestamp":1626712266000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":11,"title":["A [3Fe-4S] cluster and tRNA-dependent aminoacyltransferase BlsK in the biosynthesis of Blasticidin S"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2004-4927","authenticated-orcid":false,"given":"Xiankun","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;"},{"name":"Kobilka Institute of Innovative Drug Discovery, School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen 518172, China;"},{"name":"School of Life Sciences, University of Science and Technology of China, Hefei 230026, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchun","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaojie","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangkun","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiqin","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixin","family":"Deng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9181-8593","authenticated-orcid":false,"given":"T. Mark","family":"Zabriskie","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331-3507"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2336-7222","authenticated-orcid":false,"given":"Xinyi","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2021,7,19]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4MB00627E"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.77"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.6b00523"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201301411"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku348"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06411-x"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13888"},{"key":"e_1_3_4_8_2","first-page":"1","article-title":"Blasticidin S, a new antibiotic","volume":"11","author":"Takeuchi S.","year":"1958","unstructured":"S. Takeuchi, K. Hirayama, K. Ueda, H. Sakai, H. Yonehara, Blasticidin S, a new antibiotic. J. Antibiot. (Tokyo) 11, 1\u20135 (1958).","journal-title":"J. Antibiot. (Tokyo)"},{"key":"e_1_3_4_9_2","first-page":"126","article-title":"Chemical studies on blasticidin S","volume":"30","author":"\u014ctake N.","year":"1966","unstructured":"N. \u014ctake, S. Takeuchi, T. End\u014d, H. Yonehara, Chemical studies on blasticidin S. Agric. Biol. Chem. 30, 126\u2013141 (1966).","journal-title":"Agric. Biol. Chem."},{"key":"e_1_3_4_10_2","first-page":"667","article-title":"Inhibition of protein synthesis by blasticidin S. I. Studies with cell-free systems from bacterial and mammalian cells","volume":"57","author":"Yamaguchi H.","year":"1965","unstructured":"H. Yamaguchi, C. Yamamoto, N. Tanaka, Inhibition of protein synthesis by blasticidin S. I. Studies with cell-free systems from bacterial and mammalian cells. J. Biochem. 57, 667\u2013677 (1965).","journal-title":"J. Biochem."},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a128489"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(00)00210-3"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03254-12"},{"key":"e_1_3_4_14_2","first-page":"1292","article-title":"Studies on the biosynthesis of blasticidin S: Part I. Precursors of blasticidin S biosynthesis part II. Leucylblasticidin S, a metabolic intermediate of blasticidin S biosynthesis","volume":"32","author":"Seto H.","year":"1968","unstructured":"H. Seto, I. Yamaguchi, N. \u014ctake, H. Yonehara, Studies on the biosynthesis of blasticidin S: Part I. Precursors of blasticidin S biosynthesis part II. Leucylblasticidin S, a metabolic intermediate of blasticidin S biosynthesis. Agric. Biol. Chem. 32, 1292\u20131305 (1968).","journal-title":"Agric. Biol. Chem."},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200300583"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(99)01060-1"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00225a033"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01565"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep17641"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2016.03.023"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2017.03.017"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki408"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2010.10.016"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5326.653"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1109539108"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03797"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja1111173"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708957105"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6SC01389A"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00725-16"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.044529.114"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b07111"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007750050002"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1615732113"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio0406-171"},{"key":"e_1_3_4_36_2","first-page":"1","article-title":"PCR targeting system in Streptomyces coelicolor A3 (2)","volume":"3","author":"Gust B.","year":"2002","unstructured":"B. Gust, T. Kieser, K. Chater, PCR targeting system in Streptomyces coelicolor A3 (2). John Innes Centre 3, 1\u201339 (2002).","journal-title":"John Innes Centre"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.2102318118","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2102318118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T07:38:53Z","timestamp":1649835533000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2102318118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,19]]},"references-count":36,"journal-issue":{"issue":"30","published-print":{"date-parts":[[2021,7,27]]}},"alternative-id":["10.1073\/pnas.2102318118"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2102318118","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,19]]},"assertion":[{"value":"2021-07-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2102318118"}}