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This fundamental cellular mechanism is crucial to maintaining a functional proteome and avoiding deleterious misfolding. Here, we study how disordered nascent chains emerge from the ribosome exit tunnel, and find that interactions with the ribosome surface dominate their dynamics in\u00a0vitro and in\u00a0vivo. Moreover, we show that the types of amino acids that mediate such interactions are also those that recruit trigger factor. This lays the foundation to describe how nascent chains are handed over from the ribosome surface to chaperones during biosynthesis within the crowded cytosol.<\/jats:p>","DOI":"10.1073\/pnas.2103015118","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T16:25:29Z","timestamp":1640017529000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":36,"title":["Common sequence motifs of nascent chains engage the ribosome surface and trigger factor"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5280-230X","authenticated-orcid":false,"given":"Annika","family":"Deckert","sequence":"first","affiliation":[{"name":"Institute of Structural and Molecular Biology, University College London, London WC1E 6BT, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3963-082X","authenticated-orcid":false,"given":"Ana\u00efs M. 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