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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,7,6]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Pulmonary embolism and stroke are thromboembolic diseases affecting &gt;1 million people annually worldwide. Thromboembolism involves clot fragments affecting vital downstream organs such as the lung or brain. The mechanisms underpinning thromboembolism are not understood and require clarification in order to devise more effective treatments. Here, we developed a thromboembolism protocol supported by state-of-the-art in vivo imaging, coupled to a genetically modified murine model of reduced clot strength caused by mutations in the cross-linking sites of fibrin, which provides the mechanical scaffold of the clot. We find that fibrin \u03b3-chain cross-linking is essential for clot stability and reduces embolism. These findings introduce an important concept indicating that maintenance of clot stability during thrombosis treatment is essential to prevent thromboembolism.<\/jats:p>","DOI":"10.1073\/pnas.2103226118","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T17:09:57Z","timestamp":1624900197000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":32,"title":["Elimination of fibrin \u03b3-chain cross-linking by FXIIIa increases pulmonary embolism arising from murine inferior vena cava thrombi"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4870-6542","authenticated-orcid":false,"given":"C\u00e9dric","family":"Duval","sequence":"first","affiliation":[{"name":"Leeds Thrombosis Collective, Discovery &amp; Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds LS2 9NL, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adomas","family":"Baranauskas","sequence":"additional","affiliation":[{"name":"Leeds Thrombosis Collective, Discovery &amp; 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Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds LS2 9NL, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaher","family":"Raslan","sequence":"additional","affiliation":[{"name":"Leeds Thrombosis Collective, Discovery &amp; Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds LS2 9NL, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc A.","family":"Bailey","sequence":"additional","affiliation":[{"name":"Leeds Thrombosis Collective, Discovery &amp; Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds LS2 9NL, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7844-3600","authenticated-orcid":false,"given":"Richard M.","family":"Cubbon","sequence":"additional","affiliation":[{"name":"Leeds Thrombosis Collective, Discovery &amp; Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds LS2 9NL, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon D.","family":"Connell","sequence":"additional","affiliation":[{"name":"School of Physics and Astronomy, University of Leeds, Leeds LS2 3AR, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1636-3725","authenticated-orcid":false,"given":"Ramzi A.","family":"Ajjan","sequence":"additional","affiliation":[{"name":"Leeds Thrombosis Collective, Discovery &amp; Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds LS2 9NL, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9199-4201","authenticated-orcid":false,"given":"Helen","family":"Philippou","sequence":"additional","affiliation":[{"name":"Leeds Thrombosis Collective, Discovery &amp; 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