{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T15:17:26Z","timestamp":1789226246945,"version":"build-2803163510"},"reference-count":54,"publisher":"National Academy of Sciences","issue":"24","license":[{"start":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T00:00:00Z","timestamp":1654560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"The Center for Biomedicla Discovery at Mayo Clinic","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]},{"name":"The Center for Clinical and Translational Sciences","award":["UL1TR002377"],"award-info":[{"award-number":["UL1TR002377"]}]},{"name":"Robert and Arlene Kogod Professorship in Geriatric Medicine","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,6,14]]},"abstract":"<jats:p>\n                    Skeletal muscle atrophy is commonly associated with aging, immobilization, muscle unloading, and congenital myopathies. Generation of mature muscle cells from skeletal muscle satellite cells (SCs) is pivotal in repairing muscle tissue. Exercise therapy promotes muscle hypertrophy and strength. Primary cilium is implicated as the mechanical sensor in some mammalian cells, but its role in skeletal muscle cells remains vague. To determine mechanical sensors for exercise-induced muscle hypertrophy, we established three SC-specific cilium dysfunctional mouse models\u2014\n                    <jats:italic>Myogenic factor 5<\/jats:italic>\n                    (\n                    <jats:italic>Myf5<\/jats:italic>\n                    )\n                    <jats:italic>-Arf-like Protein 3<\/jats:italic>\n                    (\n                    <jats:italic>Arl3<\/jats:italic>\n                    )\n                    <jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      , Paired box protein Pax-7\n                    <\/jats:italic>\n                    (\n                    <jats:italic>Pax7<\/jats:italic>\n                    )-\n                    <jats:italic>Intraflagellar transport protein 88 homolog<\/jats:italic>\n                    (\n                    <jats:italic>Ift88<\/jats:italic>\n                    )\n                    <jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    , and\n                    <jats:italic>\n                      Pax7-Arl3\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    \u2014by specifically deleting a ciliary protein ARL3 in MYF5-expressing SCs, or IFT88 in PAX7-expressing SCs, or ARL3 in PAX7-expressing SCs, respectively. We show that the\n                    <jats:italic>\n                      Myf5-Arl3\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice develop grossly the same as WT mice. Intriguingly, mechanical stimulation-induced muscle hypertrophy or myoblast differentiation is abrogated in\n                    <jats:italic>\n                      Myf5-Arl3\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      Pax7-Arl3\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice or primary isolated\n                    <jats:italic>\n                      Myf5-Arl3\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      Pax7-Ift88\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    myoblasts, likely due to defective cilia-mediated Hedgehog (Hh) signaling. Collectively, we demonstrate SC cilia serve as mechanical sensors and promote exercise-induced muscle hypertrophy via Hh signaling pathway.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2103615119","type":"journal-article","created":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T16:28:44Z","timestamp":1654619324000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":21,"title":["Primary cilia in satellite cells are the mechanical sensors for muscle hypertrophy"],"prefix":"10.1073","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3341-9265","authenticated-orcid":false,"given":"Weijun","family":"Li","sequence":"first","affiliation":[{"name":"Division of Geriatric Medicine &amp; Gerontology, Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905"},{"name":"Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905"},{"name":"Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, 310030 Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2292-949X","authenticated-orcid":false,"given":"Zhenhong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine &amp; Gerontology, Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905"},{"name":"Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905"},{"name":"Orthopaedics Department, Shanghai Sixth People\u2019s Hospital, Shanghai Jiaotong University, 200240 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6405-8330","authenticated-orcid":false,"given":"Kai","family":"He","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9347-3565","authenticated-orcid":false,"given":"Xiaoyu","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8533-9438","authenticated-orcid":false,"given":"Robert J.","family":"Pignolo","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine &amp; Gerontology, Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905"},{"name":"Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3040-9424","authenticated-orcid":false,"given":"Gary C.","family":"Sieck","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghua","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2340-0739","authenticated-orcid":false,"given":"Haitao","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine &amp; Gerontology, Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905"},{"name":"Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c100059"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4295"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1999.87.3.1003"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00536"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-2545"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nrendo.2017.32","article-title":"Metabolism: Exercise resistance decoded","volume":"13","author":"Holmes D.","year":"2017","unstructured":"D. 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