{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:29:23Z","timestamp":1792625363284,"version":"4.5.0"},"reference-count":77,"publisher":"National Academy of Sciences","issue":"32","license":[{"start":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T00:00:00Z","timestamp":1644019200000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R37 AI127518"],"award-info":[{"award-number":["R37 AI127518"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI144094"],"award-info":[{"award-number":["R01 AI144094"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U01 AI125250"],"award-info":[{"award-number":["U01 AI125250"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,8,10]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Group 2 innate lymphoid cells (ILC2s) and adaptive CD4\n                    <jats:sup>+<\/jats:sup>\n                    T helper type 2 (Th2) cells express a common effector program orchestrated by the \u201cmaster\u201d transcription factor GATA3 that is acquired through development or differentiation, respectively. To elucidate the regulatory mechanisms controlling the acquisition of this shared program, we used a combination of chromatin accessibility data and CRISPR\/Cas9-mediated deletion, which revealed a\n                    <jats:italic>Gata3<\/jats:italic>\n                    enhancer necessary for ILC2 development and function. Notably, this enhancer was largely dispensable for Th2 cell differentiation. Thus, ILC2s and Th2 cells display different requirements for the induction of a common type 2 helper effector program.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2106311118","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T16:25:42Z","timestamp":1628180742000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":43,"title":["A\n                    <i>Gata3<\/i>\n                    enhancer necessary for ILC2 development and function"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2718-7971","authenticated-orcid":false,"given":"Darshan N.","family":"Kasal","sequence":"first","affiliation":[{"name":"Committee on Immunology, University of Chicago, Chicago, IL 60637;"},{"name":"Department of Pathology, University of Chicago, Chicago, IL 60637;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhitao","family":"Liang","sequence":"additional","affiliation":[{"name":"Committee on Immunology, University of Chicago, Chicago, IL 60637;"},{"name":"Department of Pathology, University of Chicago, Chicago, IL 60637;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8303-6188","authenticated-orcid":false,"given":"Maile K.","family":"Hollinger","sequence":"additional","affiliation":[{"name":"Committee on Immunology, University of Chicago, Chicago, IL 60637;"},{"name":"Department of Medicine, Section of Pulmonary and Critical Care, University of Chicago, Chicago, IL 60637;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8802-6683","authenticated-orcid":false,"given":"Crystal Y.","family":"O\u2019Leary","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Chicago, Chicago, IL 60637;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2623-5185","authenticated-orcid":false,"given":"Wioletta","family":"Lisicka","sequence":"additional","affiliation":[{"name":"Committee on Immunology, University of Chicago, Chicago, IL 60637;"},{"name":"Department of Medicine, Section of Gastroenterology, University of Chicago, Chicago, IL 60637"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne I.","family":"Sperling","sequence":"additional","affiliation":[{"name":"Committee on Immunology, University of Chicago, Chicago, IL 60637;"},{"name":"Department of Medicine, Section of Pulmonary and Critical Care, University of Chicago, Chicago, IL 60637;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1859-206X","authenticated-orcid":false,"given":"Albert","family":"Bendelac","sequence":"additional","affiliation":[{"name":"Committee on Immunology, University of Chicago, Chicago, IL 60637;"},{"name":"Department of Pathology, University of Chicago, Chicago, IL 60637;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2021,8,5]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.07.017"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.04.029"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3582"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2014.04.002"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01571"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090934"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/384474a0"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00328-5"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2623"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.34.21597"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80240-8"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1128"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20131038"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.01.012"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3318"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.06.020"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1217158110"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20200487"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.12.001"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13047"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.05065-11"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0995-40"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8575"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.2.1558"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.09.030"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00302-18"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24677"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI83894"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.02.033"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.01.011"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02132"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1008-1091"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.08.012"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/imcb.12408"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2712"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3344"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23774-9"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906988106"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbaexp.2005.01.012"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.06.006"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.08.007"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101212"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300379"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20121964"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12526"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.07.050"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6786-5_12"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11132"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1500020"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.02.002"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247606"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00053-17"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1201042109"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20171127"},{"key":"e_1_3_4_55_2","first-page":"1","article-title":"Runx\/Cbf\u03b2 complexes protect group 2 innate lymphoid cells from exhausted-like hyporesponsiveness during allergic airway inflammation","volume":"10","author":"Miyamoto C.","year":"2019","unstructured":"C. Miyamoto ., Runx\/Cbf\u03b2 complexes protect group 2 innate lymphoid cells from exhausted-like hyporesponsiveness during allergic airway inflammation. Nat. Commun. 10, 1\u201313 (2019).","journal-title":"Nat. Commun."},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20190972"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2743"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.06.003"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbn016"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80156-9"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.05.010"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201100121"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2991"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature24271"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2005.03.003"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20379"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019507108"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80142-9"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.12.7276"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-041015-055549"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12705"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3294"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200205)32:5<1428::AID-IMMU1428>3.0.CO;2-0"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4396"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb2129s109"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.05.004"},{"key":"e_1_3_4_77_2","unstructured":"D. N. Kasal A. Bendelac A Gata3 enhancer necessary for ILC2 development and function. Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE169542. Deposited 24 March 2021."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.2106311118","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2106311118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T07:28:05Z","timestamp":1649834885000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2106311118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,5]]},"references-count":77,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2021,8,10]]}},"alternative-id":["10.1073\/pnas.2106311118"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2106311118","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,5]]},"assertion":[{"value":"2021-08-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2106311118"}}