{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T07:48:54Z","timestamp":1793087334332,"version":"4.5.0"},"reference-count":61,"publisher":"National Academy of Sciences","issue":"45","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"vor","delay-in-days":181,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-15-CE15-0002-01"],"award-info":[{"award-number":["ANR-15-CE15-0002-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-14-IFEC-0006-01"],"award-info":[{"award-number":["ANR-14-IFEC-0006-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-14-IFEC-0006-01"],"award-info":[{"award-number":["ANR-14-IFEC-0006-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M&#x00E9;dicale","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,11,9]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Type IV pili (T4P) are among the most widespread adhesive factors in prokaryotes. In pathogenic\n                    <jats:italic>Neisseria<\/jats:italic>\n                    , the major pilin, which provides the structural framework for the filamentous T4P, undergoes antigenic variation allowing the bacteria to evade the humoral immune response without impacting host-cell adhesion. Here, we show that a minor pilin, PilV, is distributed throughout the pilus and contributes to\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    adhesion and that antibodies to PilV block meningococcal adhesion in vivo. Our results provide a mechanism whereby\n                    <jats:italic>N. meningitidis<\/jats:italic>\n                    varies its immunodominant major pilin to escape antibody recognition while maintaining conserved sites throughout the pilus for host receptor binding. They further suggest a strategy to prevent or block deadly\n                    <jats:italic>N. meningitidis<\/jats:italic>\n                    infections by targeting this minor pilin.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2109364118","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T16:55:29Z","timestamp":1635785729000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":39,"title":["The minor pilin PilV provides a conserved adhesion site throughout the antigenically variable meningococcal type IV pilus"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0603-6755","authenticated-orcid":false,"given":"Jean-Philippe","family":"Barnier","sequence":"first","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"},{"name":"Service de Microbiologie, Assistance Publique\u2013H\u00f4pitaux de Paris, Centre\u2013Universit\u00e9 de Paris, H\u00f4pital Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6144-927X","authenticated-orcid":false,"given":"Julie","family":"Meyer","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7469-2298","authenticated-orcid":false,"given":"Subramania","family":"Kolappan","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 3Y6, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haniaa","family":"Bouzinba-S\u00e9gard","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1016, CNRS UMR 8104, Institut Cochin, Paris 75014, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00ebl","family":"Gesbert","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1902-6943","authenticated-orcid":false,"given":"Anne","family":"Jamet","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"},{"name":"Service de Microbiologie, Assistance Publique\u2013H\u00f4pitaux de Paris, Centre\u2013Universit\u00e9 de Paris, H\u00f4pital Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Frapy","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3662-1768","authenticated-orcid":false,"given":"Sophia","family":"Sch\u00f6nherr-Hellec","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6041-6724","authenticated-orcid":false,"given":"Elena","family":"Capel","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zo\u00e9","family":"Virion","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Dupuis","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Bille","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"},{"name":"Service de Microbiologie, Assistance Publique\u2013H\u00f4pitaux de Paris, Centre\u2013Universit\u00e9 de Paris, H\u00f4pital Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Morand","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"},{"name":"Service de Bact\u00e9riologie, Assistance Publique\u2013H\u00f4pitaux de Paris, Centre\u2013Universit\u00e9 de Paris, H\u00f4pital Cochin, Paris 75014, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taliah","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Service de Chirurgie Reconstructrice et Plastique, Groupe Hospitalier Paris Saint-Joseph, Paris 75014, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2852-4765","authenticated-orcid":false,"given":"Sandrine","family":"Bourdoulous","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1016, CNRS UMR 8104, Institut Cochin, Paris 75014, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Nassif","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"},{"name":"Service de Microbiologie, Assistance Publique\u2013H\u00f4pitaux de Paris, Centre\u2013Universit\u00e9 de Paris, H\u00f4pital Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8515-9915","authenticated-orcid":false,"given":"Lisa","family":"Craig","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 3Y6, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7655-9685","authenticated-orcid":false,"given":"Mathieu","family":"Coureuil","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Paris, Paris 75006, France;"},{"name":"INSERM U1151, CNRS UMR 8253, Institut Necker Enfants-Malades, Paris 75015, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2021,11]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"237","article-title":"Meningococcal disease: Changes in epidemiology and prevention","volume":"4","author":"Chang Q.","year":"2012","unstructured":"Q. Chang , Y. L. Tzeng , D. S. Stephens , Meningococcal disease: Changes in epidemiology and prevention. Clin. Epidemiol. 4, 237\u2013245 (2012).","journal-title":"Clin. Epidemiol."},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40121-019-00262-9"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70251-6"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13063"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00494-19"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2018.02.002"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009299"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2017.1391446"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0395-8"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175653"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00321.x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-253"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3563"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit301"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0195-4"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.PSIB-0006-2018"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.616904"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.676106"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006109"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18803-z"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/378032a0"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2006.07.004"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13015"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2017.07.016"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1919567117"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12685-6"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15764"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04591.x"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2016.04.001"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218832110"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.04.065"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.261574998"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000314"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00423-12"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201488031"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1817757116"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.07.060"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006429"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04372.x"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00935.x"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911328107"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00369-12"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707581104"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07871-11"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2012.02.017"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(87)90005-2"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.2.507"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-3458fje"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts565"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi0313s48"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.849004"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1047-8477(03)00048-0"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807057822"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910048675"},{"key":"e_1_3_4_55_2","first-page":"760","article-title":"The CCP4 suite: Programs for protein crystallography","volume":"50","year":"1994","unstructured":"The CCP4 suite: Programs for protein crystallography. Acta Crystallogr. D50, 760\u2013763 (1994).","journal-title":"Acta Crystallogr."},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444900005072"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444999000839"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm216"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.2109364118","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2109364118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T07:42:15Z","timestamp":1649835735000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2109364118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":61,"journal-issue":{"issue":"45","published-print":{"date-parts":[[2021,11,9]]}},"alternative-id":["10.1073\/pnas.2109364118"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2109364118","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"2021-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2109364118"}}