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Molecular dynamics simulations reveal the negatively charged ED-domain in SUR2B, a stretch of 15 glutamate (E) and aspartate (D) residues not previously resolved, play a key MgADP-dependent role in mediating interactions at the interface between the SUR2B and Kir6.1 subunits. Our findings provide a mechanistic understanding of how channel activity is regulated by intracellular MgADP.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2109441118","type":"journal-article","created":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T16:20:25Z","timestamp":1635438025000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":50,"title":["Vascular K\n                    <sub>ATP<\/sub>\n                    channel structural dynamics reveal regulatory mechanism by Mg-nucleotides"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6553-6373","authenticated-orcid":false,"given":"Min Woo","family":"Sung","sequence":"first","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, School of Medicine, Oregon Health and Science University, Portland, OR 97239;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongying","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, School of Medicine, Oregon Health and Science University, Portland, OR 97239;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camden M.","family":"Driggers","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, School of Medicine, Oregon Health and Science University, Portland, OR 97239;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce L.","family":"Patton","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, School of Medicine, Oregon Health and Science University, Portland, OR 97239;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barmak","family":"Mostofian","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2813-6554","authenticated-orcid":false,"given":"John D.","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7662-2031","authenticated-orcid":false,"given":"Daniel M.","family":"Zuckerman","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, OR 97239"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8230-8820","authenticated-orcid":false,"given":"Show-Ling","family":"Shyng","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, School of Medicine, Oregon Health and Science University, Portland, OR 97239;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2021,10,28]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04711"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.1.227"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00003.2015"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.60.1.667"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.4.C799"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.259.1.C3"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/336382a0"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.114.03116"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.119.007322"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2012.04.014"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2324"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.c.31753"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(98)01225-9"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5391.1141"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2013.00404"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5391.1138"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(99)00050-9"},{"key":"e_1_3_4_18_2","first-page":"1","article-title":"K(ATP) channel action in vascular tone regulation: From genetics to diseases","volume":"64","author":"Shi W. 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