{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:27:18Z","timestamp":1792477638022,"version":"4.5.0"},"reference-count":60,"publisher":"National Academy of Sciences","issue":"15","license":[{"start":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:00:00Z","timestamp":1648598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Qatar Biomedical Research Institute, Internal Grant","award":["VR98"],"award-info":[{"award-number":["VR98"]}]},{"name":"Alzheimer&amp;amp;amp;apos;s Research UK Fellowship","award":["ARUK-RF2018C-005"],"award-info":[{"award-number":["ARUK-RF2018C-005"]}]},{"DOI":"10.13039\/501100003554","name":"Lundbeck Foundation","doi-asserted-by":"crossref","award":["R223-2015-4222"],"award-info":[{"award-number":["R223-2015-4222"]}],"id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,4,12]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Converging evidence points to the build-up of phosphorylated \u03b1-synuclein (\u03b1-syn) at residue serine 129 (pS129) in Lewy body disease, suggesting its central role in the regulation of \u03b1-syn aggregation and neuronal degeneration. However, a comprehensive understanding of the role of \u03b1-syn phosphorylation at pS129 in \u03b1-synuclenopathies pathogenesis is still lacking. Herein, we study the phosphorylation incidence and its effect on \u03b1-syn aggregation propensity and cellular toxicity. Collectively, our data suggest that pS129 occurred subsequent to initial \u03b1-syn aggregation, lessened aggregation propensity, and attenuated cytotoxicity through diverse assays. Our findings highlight major implications for a better understanding of the role of a molecular modification on protein aggregation.<\/jats:p>","DOI":"10.1073\/pnas.2109617119","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T14:44:40Z","timestamp":1648651480000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":192,"title":["\u03b1-Synuclein phosphorylation at serine 129 occurs after initial protein deposition and inhibits seeded fibril formation and toxicity"],"prefix":"10.1073","volume":"119","author":[{"given":"Simona S.","family":"Ghanem","sequence":"first","affiliation":[{"name":"Neurological Disorders Research Center, Qatar Biomedical Research Institute, Hamad Bin Khalifa University, Qatar Foundation, Doha 34110, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nour K.","family":"Majbour","sequence":"additional","affiliation":[{"name":"Neurological Disorders Research 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