{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:36:51Z","timestamp":1790905011165,"version":"4.1.0"},"reference-count":57,"publisher":"National Academy of Sciences","issue":"15","license":[{"start":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T00:00:00Z","timestamp":1649376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC: 31571077"],"award-info":[{"award-number":["NSFC: 31571077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC: 32000553"],"award-info":[{"award-number":["NSFC: 32000553"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2020M683033"],"award-info":[{"award-number":["2020M683033"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004000","name":"Guangzhou Science and Technology Program key projects","doi-asserted-by":"publisher","award":["201707020009"],"award-info":[{"award-number":["201707020009"]}],"id":[{"id":"10.13039\/501100004000","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21976216"],"award-info":[{"award-number":["21976216"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,4,12]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>The connecting cilium (CC) of the photoreceptor provides the only route for the trafficking of the outer segment (OS) proteins. Failure of OS protein transport causes degenerative photoreceptor diseases, including retinitis pigmentosa. We demonstrate that Tmem138, a protein linked to ciliopathy, is localized to the photoreceptor CC. Germline deletion of Tmem138 abolished OS morphogenesis, followed by rapid photoreceptor degeneration. Tmem138 interacts with rhodopsin and two additional CC compartment proteins, Ahi1 and Tmem231, likely forming a membrane complex to facilitate trafficking of rhodopsin and other OS-bound proteins across the CC. The study thus implicates a new line of regulation on the delivery of OS proteins through interactions with CC membrane complex(es) and provides insights into photoreceptor ciliopathy diseases.<\/jats:p>","DOI":"10.1073\/pnas.2109934119","type":"journal-article","created":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T14:14:36Z","timestamp":1649427276000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":21,"title":["Tmem138 is localized to the connecting cilium essential for rhodopsin localization and outer segment biogenesis"],"prefix":"10.1073","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7182-9164","authenticated-orcid":false,"given":"Dianlei","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Ru","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijing","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjuan","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1307-2153","authenticated-orcid":false,"given":"Yingchun","family":"Su","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyong","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1802-7917","authenticated-orcid":false,"given":"Shujuan","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9243-9923","authenticated-orcid":false,"given":"Bin","family":"Zou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Wei","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2086-5805","authenticated-orcid":false,"given":"Xialin","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunqiao","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"},{"name":"Guangdong Province Key Laboratory of Brain Function and Disease, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China"},{"name":"Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2022,4,8]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/75614"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-10-05818.1994"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.519"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.14176"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2013.08.004"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e10-09-0792"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2013.03.002"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2015.02.007"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0015-12.2012"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00023-4"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200107108"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.05.049"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.042308.113337"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02665"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-531"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201012116"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002416"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201488044"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/2046-2530-1-22"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2018.12.004"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv123"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201411087"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2011.11.005"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-8766"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3273"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.891"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1213506"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/cge.12836"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.420786"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200707181"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1042\/BC20110034"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2034-08.2009"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201608081"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-8635"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00531-1"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1509285113"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1761-18.2018"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA117.000141"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2012-101132"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2016.05.003"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1282-14.2014"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.638437"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-15-06267.1999"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0169(200006)46:2<95::AID-CM2>3.0.CO;2-Q"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1903125117"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902880410"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098939"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3437-13.2014"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19971013)387:1<148::AID-CNE12>3.0.CO;2-Q"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.2.736"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0297-216"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2410"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901900307"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13630-015-0013-1"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.06.030"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901880205"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01191508"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2109934119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T07:47:13Z","timestamp":1649836033000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2109934119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,8]]},"references-count":57,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2022,4,12]]}},"alternative-id":["10.1073\/pnas.2109934119"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2109934119","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,8]]},"assertion":[{"value":"2021-06-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2109934119"}}