{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T11:18:43Z","timestamp":1785323923747,"version":"3.55.0"},"reference-count":49,"publisher":"National Academy of Sciences","issue":"38","license":[{"start":{"date-parts":[[2022,3,13]],"date-time":"2022-03-13T00:00:00Z","timestamp":1647129600000},"content-version":"vor","delay-in-days":181,"URL":"https:\/\/www.pnas.org\/site\/aboutpnas\/licenses.xhtml"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI088204"],"award-info":[{"award-number":["AI088204"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000041","name":"American Diabetes Association","doi-asserted-by":"publisher","award":["7-12-MN-51"],"award-info":[{"award-number":["7-12-MN-51"]}],"id":[{"id":"10.13039\/100000041","id-type":"DOI","asserted-by":"publisher"}]},{"name":"DBT\/Wellcome Trust India Alliance","award":["IA\/I\/19\/1\/504276"],"award-info":[{"award-number":["IA\/I\/19\/1\/504276"]}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM007753"],"award-info":[{"award-number":["T32GM007753"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,9,21]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Aire controls immunological tolerance by driving promiscuous expression of a large swath of the genome in medullary thymic epithelial cells (mTECs). Its molecular mechanism remains enigmatic. To address the hypothesis that Aire impacts chromatin organization through widespread promotion of superenhancer\u2013promoter loops, we performed genome-wide, high-resolution chromosome-conformation capture (Hi-C) experiments on ex vivo mTECs from thymi of mice expressing Aire or not. Integration of the resulting data with inventories of the genome-wide locations of Aire, subunits of the mediator and cohesin complexes, and the chromatin-domain enforcer, CCCTC-binding factor (CTCF), indeed argued for a looping model, which was further supported by biochemical and in vivo loss-of-function analyses.<\/jats:p>","DOI":"10.1073\/pnas.2110991118","type":"journal-article","created":{"date-parts":[[2021,9,17]],"date-time":"2021-09-17T13:57:28Z","timestamp":1631887048000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":28,"title":["Aire regulates chromatin looping by evicting CTCF from domain boundaries and favoring accumulation of cohesin on superenhancers"],"prefix":"10.1073","volume":"118","author":[{"given":"Kushagra","family":"Bansal","sequence":"first","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115;"},{"name":"Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women\u2019s Hospital, Boston, MA 02115;"},{"name":"Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Michelson","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115;"},{"name":"Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women\u2019s Hospital, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1259-5406","authenticated-orcid":false,"given":"Ricardo N.","family":"Ramirez","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115;"},{"name":"Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women\u2019s Hospital, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron D.","family":"Viny","sequence":"additional","affiliation":[{"name":"Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065;"},{"name":"Center for Hematologic Malignancies, Memorial Sloan Kettering Cancer Center, New York, NY 10065;"},{"name":"Leukemia Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065;"},{"name":"Center for Epigenetics Research, Memorial Sloan Kettering Cancer Center, New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross L.","family":"Levine","sequence":"additional","affiliation":[{"name":"Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065;"},{"name":"Center for Hematologic Malignancies, Memorial Sloan Kettering Cancer Center, New York, NY 10065;"},{"name":"Leukemia Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065;"},{"name":"Center for Epigenetics Research, Memorial Sloan Kettering Cancer Center, New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Benoist","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115;"},{"name":"Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women\u2019s Hospital, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Mathis","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115;"},{"name":"Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women\u2019s Hospital, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2021,9,13]]},"reference":[{"key":"e_1_3_5_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-051116-052320"},{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12714"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3675"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01085-07"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1119351109"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1512081112"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.09.053"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2020.03.003"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.11.021"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature24281"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0234-8"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1181369"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3247"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.12.030"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1518552112"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0128-0"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature22063"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.04.013"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201798004"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.08.006"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.07.013"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.04.024"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.188300.114"},{"key":"e_1_3_5_24_2","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1242\/jcs.197236","article-title":"Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage","volume":"130","author":"Bot C.","year":"2017","unstructured":"C. Bot ., Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage. J. Cell Sci. 130, 1134\u20131146 (2017).","journal-title":"J. Cell Sci."},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1792-x"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaz3418"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.60"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-018-0070-4"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3246"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806616105"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.03.035"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.030"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10442"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2017.10.018"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707486105"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808069105"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.025"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.25776"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1080\/19491034.2017.1389365"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12593"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature21429"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.01.003"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13835"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2016.07.002"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.05.004"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky504"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1222023110"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr167"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt263"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.2110991118","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2110991118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,8]],"date-time":"2024-09-08T10:52:12Z","timestamp":1725792732000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2110991118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,13]]},"references-count":49,"journal-issue":{"issue":"38","published-print":{"date-parts":[[2021,9,21]]}},"alternative-id":["10.1073\/pnas.2110991118"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2110991118","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.740813738.793589501","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,13]]},"assertion":[{"value":"2021-08-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2110991118"}}