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In this study, we utilized APEX2 proximity-labeling techniques and identified nonmuscle myosin heavy chain IIA (MYH9) as an ACE2 coreceptor to promote SARS-CoV-2 infection of human lung cells, as well as infection of pan-coronavirus. We demonstrated that MYH9 colocalized with SARS-CoV-2 S primarily at the membrane, which interacts with the S2 subunit and the S1-NTD subunit through its C-terminal domain, and enhances SARS-CoV-2 entry in an ACE2-dependent manner. Our results define MYH9 as a key host factor that facilitates SARS-CoV-2 and pan-coronavirus infection, which may serve as a potential target for future clinical intervention strategies.<\/jats:p>","DOI":"10.1073\/pnas.2111011118","type":"journal-article","created":{"date-parts":[[2021,12,6]],"date-time":"2021-12-06T18:15:20Z","timestamp":1638814520000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":55,"title":["Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells"],"prefix":"10.1073","volume":"118","author":[{"given":"Jian","family":"Chen","sequence":"first","affiliation":[{"name":"Zhongshan Hospital, Institutes of Biomedical Sciences, Fudan University, Shanghai 201508, China;"},{"name":"Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, 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