{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T19:56:13Z","timestamp":1793476573196,"version":"4.5.0"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"48","license":[{"start":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T00:00:00Z","timestamp":1637539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2021,11,30]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Significant calcium absorption across renal and intestinal epithelia occurs via the paracellular pathway. However, the identity of the paracellular pore involved is unknown. Claudin-2 and claudin-12 contribute paracellular calcium permeability in cell models, but single knockout animals don\u2019t have altered serum calcium or bone mineralization. To investigate this,\n                    <jats:italic>Cldn2\/12<\/jats:italic>\n                    double knockout mice were generated. They display decreased intestinal calcium absorption and renal calcium wasting, resulting in hypocalcemia and markedly reduced bone mineralization. Claudin-2 and claudin-12 don\u2019t physically interact in\u00a0vitro, and coexpression has an additive effect on calcium permeability. Our work identifies claudin-2 and claudin-12 as important constituents of the paracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    pathway in intestine and kidney enabling calcium transport and highlights their important complementary roles in maintaining calcium homeostasis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2111247118","type":"journal-article","created":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T16:26:54Z","timestamp":1637598414000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":66,"title":["Claudin-2 and claudin-12 form independent, complementary pores required to maintain calcium homeostasis"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2690-1785","authenticated-orcid":false,"given":"Megan R.","family":"Beggs","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Alberta, Edmonton, AB, T6G 2H7, Canada;"},{"name":"Women\u2019s and Children\u2019s Health Research Institute, Edmonton, AB, T6G 1C9, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kennedi","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Alberta, Edmonton, AB, T6G 2H7, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanling","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Alberta, Edmonton, AB, T6G 2H7, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debbie D.","family":"O\u2019Neill","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Alberta, Edmonton, AB, T6G 2H7, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Saurette","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Alberta, Edmonton, AB, T6G 2H7, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9841-9499","authenticated-orcid":false,"given":"Allein","family":"Plain","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Alberta, Edmonton, AB, T6G 2H7, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juraj","family":"Rievaj","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Alberta, Edmonton, AB, T6G 2H7, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Doschak","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy &amp; Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2H5, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Cordat","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Alberta, Edmonton, AB, T6G 2H7, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9170-2168","authenticated-orcid":false,"given":"Henrik","family":"Dimke","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark;"},{"name":"Department of Nephrology, Odense University Hospital, 5000 Odense, Denmark;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7396-7894","authenticated-orcid":false,"given":"R. 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