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Here, we engineered rSARS-CoV-2 expressing fluorescent (Venus) or luciferase (Nano luciferase) reporter genes from the viral nucleocapsid (N) locus, without deleting any viral gene. These novel reporter-expressing rSARS-CoV-2, which give a higher expression level of reporter genes, allow us to monitor SARS-CoV-2 replication dynamic both in vitro and in vivo. These new reporter-expressing rSARS-CoVs-2 represent an excellent option to assess viral replication, tropisms, and pathogenicity as well as the rapid in vivo evaluation of effective countermeasures for the treatment of SARS-CoV-2 infection.<\/jats:p>","DOI":"10.1073\/pnas.2111593118","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T15:45:32Z","timestamp":1632498332000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":59,"title":["Analysis of SARS-CoV-2 infection dynamic in vivo using reporter-expressing viruses"],"prefix":"10.1073","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1934-9494","authenticated-orcid":false,"given":"Chengjin","family":"Ye","sequence":"first","affiliation":[{"name":"Host-Pathogen Interactions Program, Texas Biomedical Research Institute, San Antonio, TX 78227;"},{"name":"Population Health Program, Texas Biomedical Research Institute, San Antonio, TX 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