{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:57:52Z","timestamp":1791338272320,"version":"4.3.1"},"reference-count":22,"publisher":"National Academy of Sciences","issue":"20","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,9,25]]},"abstract":"<jats:p>\n                    The Neuronal Wiskott\u2013Aldrich syndrome protein (N-WASP) has emerged\n as a central regulator of the actin cytoskeleton with abilities to\n integrate multiple upstream signal inputs and transmit them to the\n Arp2\/3 complex. Here, we demonstrate that native N-WASP is present in\n a tight complex with a proline-rich protein, CR16, which shares \u224825%\n identity with WASP interacting protein. CR16 is encoded by a gene\n previously cloned as a glucocorticoid-regulated mRNA from a rat\n hippocampal cDNA library. Although N-WASP is expressed ubiquitously,\n full-length CR16 protein is found predominately in the brain. CR16 and\n N-WASP colocalize in primary hippocampal neurons and at the tips of\n their growth cone filopodia.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , CR16 directly\n binds both monomeric and filamentous actin but does not affect the\n kinetics of actin polymerization mediated by N-WASP and the Arp2\/3\n complex. Sequence homologues of CR16 are found not only in other\n vertebrates but also in the invertebrate\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    and in yeast.\n Thus, CR16 and WASP interacting protein belong to a family of\n N-WASP-binding proteins.\n                  <\/jats:p>","DOI":"10.1073\/pnas.211420498","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:34:10Z","timestamp":1027679650000},"page":"11306-11311","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":81,"title":["CR16 forms a complex with N-WASP in brain and is a novel member of a conserved proline-rich actin-binding protein family"],"prefix":"10.1073","volume":"98","author":[{"given":"Hsin-Yi Henry","family":"Ho","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, Boston, MA\r 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajat","family":"Rohatgi","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, Boston, MA\r 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, Boston, MA\r 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc W.","family":"Kirschner","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, Boston, MA\r 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2001,9,11]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.5.1325"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/34208"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.136.3.649"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81050-8"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00917.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000687200"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.23.6932"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80732-1"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)00015-3"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6181"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.6.1299"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.5.1125"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81200-3"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(90)90004-J"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000126932"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02736841"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.26.14671"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb00930.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/35074551"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.146.6.1319"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/35017080"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81341-0"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.211420498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T08:30:13Z","timestamp":1649838613000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.211420498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9,11]]},"references-count":22,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2001,9,25]]}},"alternative-id":["10.1073\/pnas.211420498"],"URL":"https:\/\/doi.org\/10.1073\/pnas.211420498","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,9,11]]},"assertion":[{"value":"2001-08-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-09-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}